Anti-Mouse TIM-4 Antibody (RMT4-53)

Cat. No.: HY-P990820 Purity: 95.00%: Data Sheet
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Anti-Mouse TIM-4 Antibody (RMT4-53) is a rat-derived IgG2b κ type antibody inhibitor, targeting to mouse TIM-4. Anti-Mouse TIM-4 Antibody (RMT4-53) can block T cell immunoglobulin and mucin domain 4 (TIM-4). Anti-Mouse TIM-4 Antibody (RMT4-53) can inhibit the engulfment of apoptotic cells and T cell proliferation. Anti-Mouse TIM-4 Antibody (RMT4-53) can be used for the researches of cancer, immunology and metabolic disease, such as MC38 tumor and graft.

For research use only. We do not sell to patients.

Anti-Mouse TIM-4 Antibody (RMT4-53) Chemical Structure

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Description

Isotype

Rat IgG2b kappa

Recommend Isotype Controls

Rat IgG2b kappa, Isotype Control

Species Reactivity

Mouse

IC₅₀ & Target

TIM-4

In Vitro

Anti-Mouse TIM-4 Antibody (RMT4-53) (2-4 h) inhibits T cell STAT6 activation in CD4⁺ T cells ^[1].
Anti-Mouse TIM-4 Antibody (RMT4-53) (4 h) inhibits phagocytosis of apoptotic EG7 cells in Timd4^−/− and WT peritoneal macrophages^[2].
Anti-Mouse TIM-4 Antibody (RMT4-53) (4 h) augments cross-priming of OVA-specific T cells in TIM-4⁺ bone-marrow derived macrophages (BMDMs)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Anti-Mouse TIM-4 Antibody (RMT4-53) (500 μg at days 0 and 250 μg at days 2, 4 and 6, i.p.) reduces numbers of allospecific Thy1.2⁺ T cells in both spleen and draining lymph nodes in ABMtg GFP^- CD4⁺ tg T cells transferred B6 Thy1.1⁺ mice of a bm12 skin graft^[1].
Anti-Mouse TIM-4 Antibody (RMT4-53) (500 μg at days 0 and 250 μg at days 2, 4, 6 and 8, i.p.) prolongs graft survival in B6 CD4⁺25^- T cells transferred B6 RAG^−/− mice of a bm12 skin transplant^[1].
Anti-Mouse TIM-4 Antibody (RMT4-53) (i.p., at days 8, 10 and 12) inhibits tumor growth companied with CDDP (HY-17394) in MC38 tumor Atg5^flox/flox mice^[2].
Anti-Mouse TIM-4 Antibody (RMT4-53) (0.5 mg/mouse, i.v., 1 h prior to ischemia) suppresses liver damage in segmental (70%) hepatic ischemia-reperfusion injury (IRI) mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	B6 CD4⁺25^- T cells transferred B6 RAG^−/− mice of a bm12 skin transplant^[1]
Dosage:	500 μg at days 0 and 250 μg at days 2, 4, 6 and 8
Administration:	Intraperitoneally injection
Result:	Prolonged graft survival. Increased the generation of iTregs.

Animal Model:	Segmental (70%) hepatic ischemia-reperfusion injury (IRI) mice^[3]
Dosage:	0.5 mg/mouse
Administration:	Intravenously injection, 1 h prior to ischemia
Result:	Decreased sALT levels. Ameliorated histological features of liver IRI.

Gene ID

276891 [NCBI]

Accession

Q6U7R4 / NP_848874.3

Application

ELISA, FACS, Functional assay, Research in vivo

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Molecular Weight

150 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Anti-Mouse TIM-4 Antibody (RMT4-53)]

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Purity: 95.00%

Select Batch:

Data Sheet (262 KB) SDS (251 KB)

COA (232 KB)

Inhibitory Antibodies User Guide (602 KB)

References

[1]. Yeung MY, et al. Interruption of dendritic cell-mediated TIM-4 signaling induces regulatory T cells and promotes skin allograft survival. J Immunol. 2013 Oct 15;191(8):4447-55. [Content Brief]

[2]. Baghdadi M, et al. TIM-4 glycoprotein-mediated degradation of dying tumor cells by autophagy leads to reduced antigen presentation and increased immune tolerance. Immunity. 2013 Dec 12;39(6):1070-81. [Content Brief]

[3]. Ji H, et al. T-cell immunoglobulin and mucin domain 4 (TIM-4) signaling in innate immune-mediated liver ischemia-reperfusion injury. Hepatology. 2014 Dec;60(6):2052-2064. [Content Brief]

Anti-Mouse TIM-4 Antibody (RMT4-53) Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse TIM-4 Antibody (RMT4-53)Transmembrane GlycoproteinTIM-4Apoptotic cellsT cellsMC38GraftBMDMIRIInhibitorinhibitorinhibit

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