2. Immunology/Inflammation
  3. Tim3
  4. Anti-Mouse TIM-3 Antibody (B8.2C12)

Anti-Mouse TIM-3 Antibody (B8.2C12) 

Cat. No.: HY-P990004 Purity: 99.48%
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Anti-Mouse TIM-3 Antibody (B8.2C12) is an anti-mouse TIM-3 IgG1 monoclonal antibody. Anti-Mouse TIM-3 Antibody (B8.2C12) can block the binding of Tim-3 with Phosphatidylserine (PtdSer) and CEACAM1 without interfering with the binding to Galectin-9. Anti-Mouse TIM-3 Antibody (B8.2C12) can inhibit tumor growth and activate tumor infiltrating CD8+ T cells. Anti-Mouse TIM-3 Antibody (B8.2C12) can be used for studying cancer such as breast cancer and colon cancer and constructing experimental autoimmune encephalomyelitis (EAE) models.

For research use only. We do not sell to patients.

Anti-Mouse TIM-3 Antibody (B8.2C12) Chemical Structure

Anti-Mouse TIM-3 Antibody (B8.2C12) Chemical Structure

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Description

Anti-Mouse TIM-3 Antibody (B8.2C12) is an anti-mouse TIM-3 IgG1 monoclonal antibody. Anti-Mouse TIM-3 Antibody (B8.2C12) can block the binding of Tim-3 with Phosphatidylserine (PtdSer) and CEACAM1 without interfering with the binding to Galectin-9. Anti-Mouse TIM-3 Antibody (B8.2C12) can inhibit tumor growth and activate tumor infiltrating CD8+ T cells. Anti-Mouse TIM-3 Antibody (B8.2C12) can be used for studying cancer such as breast cancer and colon cancer and constructing experimental autoimmune encephalomyelitis (EAE) models[1][2][3].

Isotype

Rat IgG1, kappa
Recommend Isotype Controls
IC50 & Target

TIM-3
In Vitro

Anti-Mouse TIM-3 Antibody (B8.2C12) (10 μg/mL, 7 days) increases Th17 cell differentiation and IL-17A production in CD4+CD25- T cells from the spleen of C57/BL6 mice[2].
Anti-Mouse TIM-3 Antibody (B8.2C12) (4 μg/mL, 3 days) promotes CD4+ T cells apoptosis, but has no significant effect on CD8+ T cells from the spleen of experimental autoimmune encephalomyelitis (EAE) mice[2].
Anti-Mouse TIM-3 Antibody (B8.2C12) (10 μg/mL) can block the binding of Tim-3 and PtdSer in thymocyte from Balb/c mice and the binding of Tim-3 to CEACAM1 in Jurkat T cells[3].
Anti-Mouse TIM-3 Antibody (B8.2C12) (10 μg/mL) does not block the binding of Tim-3 to mouse or human Galectin-9[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Mouse TIM-3 Antibody (B8.2C12) Related Antibodies

Apoptosis Analysis[2]

Cell Line: CD4+ T cells and CD8+ T cells
Concentration: 4 μg/mL
Incubation Time: 3 days
Result: Reduced the proportion of CD4+ T cells.
Had no significant effect on the proportion of CD8+ T cells.
In Vivo

Anti-Mouse TIM-3 Antibody (B8.2C12) (100 μg, i.p., on day 7, 12, and 17) significantly inhibits tumor growth and increases activated CD8+ T cells within the tumor combinated with Etoposide (ETO) (HY-13629) in female BALB/c mice bearing 4T1 tumors[1].
Anti-Mouse TIM-3 Antibody (B8.2C12) (300 μg, i.v., from day 8 post-immunization, for 5 days) successfully constructed the experimental autoimmune encephalomyelitis (EAE) model in C57/BL6 mice[2].
Anti-Mouse TIM-3 Antibody (B8.2C12) (200 μg, i.p., once every 3 days, for a total of 2 shots) may exert anti-tumor effects by blocking ligand binding or downregulating Tim-3 in wild type Balb/c mice bearing CT26 tumors[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 5×104 4T1 cells injected female BALB/c mice (6 weeks)[1]
Dosage: 100 μg, combinated with ETO (50 mg/kg, i.v., on day 6 and 18)
Administration: Intraperitoneal injection (i.p.), on day 7, 12, and 17
Result: Significantly inhibited tumor volume.
Significantly reduced cancer stem cell (CSC) frequency.
Downregulated the level of STT3 in tumor cells.
Significantly increased CD8+ IFN-γ+ T cells.
Animal Model: C57/BL6 mice (7-8 weeks)[2]
Dosage: 300 μg, combinated with MOG35-55 (HY-P3719)
Administration: Intravenous injection (i.v.), from day 8 post-immunization for 5 days
Result: Increased the proportion of CD4+ T cells and Th17 cells in the spinal cord.
Aggravated the symptoms of EAE.
Animal Model: 1×106 CT26 cells injected wild type Balb/c mice[3]
Dosage: 200 μg
Administration: Intraperitoneal injection (i.p.), once every 3 days for a total of 2 shots
Result: Did not alter the frequency of Treg cells within the tumor.
Might downregulate Tim-3 and exert anti-tumor effects.
Gene ID

171285  [NCBI]

Accession

Q8VIM0-1
Application

ELISA, FACS, Functional assay, Research in vivo
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Molecular Weight

150 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Anti-Mouse TIM-3 Antibody (B8.2C12)]
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Anti-Mouse TIM-3 Antibody (B8.2C12) Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse TIM-3 Antibody (B8.2C12)Tim3T-Cell Immunoglobulin and Mucin Containing Protein-3CD366TIM-3breast cancercolon cancer4T1CT26EAEInhibitorinhibitorinhibit

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