Anti-Mouse TIM-1/CD365 Antibody (3B3)

Cat. No.: HY-P990154: Data Sheet
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Anti-Mouse TIM-1/CD365 Antibody (3B3) is a rat-derived IgG2a κ type agonistic antibody, targeting to mouse TIM-1/CD365. Anti-Mouse TIM-1/CD365 Antibody (3B3) enhances T-cell proliferation and responses by forming a stable TIM-1 complex and bringing TIM-1 into the T-cell receptor (TCR)-CD3 complex. Anti-Mouse TIM-1/CD365 Antibody (3B3) can be used for the researches of cancer, inflammation and immunology, such as experimental autoimmune encephalomyelitis, B16 F10 tumor and transplant.

For research use only. We do not sell to patients.

Anti-Mouse TIM-1/CD365 Antibody (3B3) Chemical Structure

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Description

Isotype

Rat IgG2a kappa

Recommend Isotype Controls

Rat IgG2a kappa, Isotype Control

Species Reactivity

Mouse

IC₅₀ & Target

TIM-1/CD365

In Vitro

Anti-Mouse TIM-1/CD365 Antibody (3B3) (10 μg/mL, 48 h) enhances T cell proliferation in response to ovalbumin and antigen-presenting cells ^[1].
Anti-Mouse TIM-1/CD365 Antibody (3B3) (10 μg/mL, 3 days) increases IL-4 levels in T_H2 cells^[1].
Anti-Mouse TIM-1/CD365 Antibody (3B3) (2.5 μg/mL, 2-4 days) results in a substantial increase in CD4+ and CD8+ T cell accumulation in mixed lymphocytes^[4].
Anti-Mouse TIM-1/CD365 Antibody (3B3) (2.5 μg/mL, 6 days) enhances proliferation of CD4+CD25+ C57BL/6 regulatory T cells stimulated with mature DBA/2 DC cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Anti-Mouse TIM-1/CD365 Antibody (3B3) (100 μg, i.p., 1 d or 8 h before the initial exposure to OVA) abrogates respiratory tolerance induction and develops airway inflammation in ovalbumin (OVA)-induced airway hyperreactivity (AHR) mice models^[1].
Anti-Mouse TIM-1/CD365 Antibody (3B3) suppresses tumor growth and increases activated CD4+ and CD8+ T cells in B16 F10 tumor mice models^[2].
Anti-Mouse TIM-1/CD365 Antibody (3B3) (100 μg, i.p., every other day from day 0 to 8) enhances the severity in experimental autoimmune encephalomyelitis (EAE) mice models^[3].
Anti-Mouse TIM-1/CD365 Antibody (3B3) (250 μg, i.p., at days 0 and 2 after transplantation) negates prevent allograft rejection in MHC-mismatched islet transplant mice models^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Ovalbumin-induced airway hyperreactivity (AHR) mice models^[1]
Dosage:	100 μg
Administration:	Intraperitoneally injection, 1 d or 8 h before the initial exposure to OVA
Result:	Produced lower concentrations of IL-4 and IL-10 in splenocytes. Showed considerable airway inflammation.

Animal Model:	Experimental autoimmune encephalomyelitis (EAE) mice models (PLP139-151-immunized SJL mice) ^[3]
Dosage:	100 μg
Administration:	Intraperitoneally injection, every other day from day 0 to 8
Result:	Had 3-5 times the basal proliferation of spleen cells. Secreted large quantities of IFN-γ, IL-17, 2, 6, and TNF-α in spleen cells. Increased the frequency of IL-17-producing PLP139-151/IAs-reactive CD4+ T cells and IFN-γ–producing PLP139-151-specific CD4+ T cells. Showed large numbers of neutrophils in the lesions. Enhanced the severity of EAE.

Gene ID

286934 [NCBI]

Accession

O54947 / NP_775172.1

Molecular Weight

150 kDa

SMILES

[Anti-Mouse TIM-1/CD365 Antibody (3B3)]

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Inhibitory Antibodies User Guide (602 KB)

References

[1]. Umetsu SE, et al. TIM-1 induces T cell activation and inhibits the development of peripheral tolerance. Nat Immunol. 2005 May;6(5):447-54. [Content Brief]

[2]. Tian X, et al. B-cell immune checkpoint TIM-1: a potential target for tumour immunotherapy. Signal Transduct Target Ther. 2023 Oct 20;8(1):389. [Content Brief]

[3]. Xiao S, et al. Differential engagement of Tim-1 during activation can positively or negatively costimulate T cell expansion and effector function. J Exp Med. 2007 Jul 9;204(7):1691-702. [Content Brief]

[4]. Degauque N, et al. Immunostimulatory Tim-1-specific antibody deprograms Tregs and prevents transplant tolerance in mice. J Clin Invest. 2008 Feb;118(2):735-41. [Content Brief]

Anti-Mouse TIM-1/CD365 Antibody (3B3) Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse TIM-1/CD365 Antibody (3B3)Transmembrane GlycoproteinTIM-1CD365T cellsT-cell receptorB16 F10Experimental autoimmune encephalomyelitisCD4+ T cellsCD8+ T cellsInhibitorinhibitorinhibit

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