2. NF-κB Metabolic Enzyme/Protease
  3. RANKL/RANK E1/E2/E3 Enzyme NF-κB
  4. Anti-Mouse RANKL/CD254 Antibody (IK22/5)

Anti-Mouse RANKL/CD254 Antibody (IK22/5) 

Cat. No.: HY-P990165 Purity: 95.00%
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Anti-Mouse RANKL/CD254 Antibody (IK22/5) is an anti-mouse RANKL/CD254 IgG2a monoclonal antibody. Anti-Mouse RANKL/CD254 Antibody (IK22/5) inhibits osteoclast formation and activity by specifically blocking the binding of RANKL and RANK. Anti-Mouse RANKL/CD254 Antibody (IK22/5) can inhibit the expression of MuRF1/Trogin-1 and activation of NF-κB. Anti-Mouse RANKL/CD254 Antibody (IK22/5) can be used for research on cancer such as melanoma or colon cancer and osteoporosis.

For research use only. We do not sell to patients.

Anti-Mouse RANKL/CD254 Antibody (IK22/5)

Anti-Mouse RANKL/CD254 Antibody (IK22/5) Chemical Structure

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Description

Anti-Mouse RANKL/CD254 Antibody (IK22/5) is an anti-mouse RANKL/CD254 IgG2a monoclonal antibody. Anti-Mouse RANKL/CD254 Antibody (IK22/5) inhibits osteoclast formation and activity by specifically blocking the binding of RANKL and RANK. Anti-Mouse RANKL/CD254 Antibody (IK22/5) can inhibit the expression of MuRF1/Trogin-1 and activation of NF-κB. Anti-Mouse RANKL/CD254 Antibody (IK22/5) can be used for research on cancer such as melanoma or colon cancer and osteoporosis[1][2][3].

Isotype

Rat IgG2a kappa
Recommend Isotype Controls
Species Reactivity

Mouse
IC50 & Target[3]

NF-κB

 

In Vitro

Anti-Mouse RANKL/CD254 Antibody (IK22/5) (3-5 days) dose dependently inhibits osteoclast differentiation by blocking the RANKL/RANK interaction in mouse RANKL-transfected NRK cells[1].
Anti-Mouse RANKL/CD254 Antibody (IK22/5) (0-1000 nM, 3-5 days) drastically inhibited the formation of TRAP+ multinucleated cells in a dose-dependentmanner[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Mouse RANKL/CD254 Antibody (IK22/5) Related Antibodies
In Vivo

Anti-Mouse RANKL/CD254 Antibody (IK22/5) (300 μg/mouse, i.p., three times per week, for 14-21 days) significantly reduces bone erosion and osteoporosis in male DBA/1 mice with collagen induced arthritis (CIA)[1].
Anti-Mouse RANKL/CD254 Antibody (IK22/5) (200 μg, i.p., on day -1, 0, and 2) significantly reduces the number of lung metastatic nodules combined with anti-CTLA4 mAb in C57BL/6 wild-type (WT) mice bearing B16F10 or RM-1 lung metastasis[2].
Anti-Mouse RANKL/CD254 Antibody (IK22/5) (200 μg, i.p., once every 2-4 days, from day 3 to 12) significantly inhibits tumor growth combined with anti-CTLA4 mAb in C57BL/6 WT mice bearing B16F10 tumors or CT26 tumors[2].
Anti-Mouse RANKL/CD254 Antibody (IK22/5) (100 or 250 μg, i.p., twice weekly, for 24 weeks) significantly improves muscle atrophy and its function in female C57BL/6 mice with a cigarette smoke (CS) exposure induced chronic obstructive pulmonary disease (COPD) model[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Bovine type II collagen (200 μg) (HY-NP102) injected male DBA/1 mice (6 weeks)[1]
Dosage: 300 μg/mouse
Administration: Intraperitoneal injection (i.p.), three times per week for 14-21 days
Result: Bringed bone density close to normal levels and preserved joint structures intact.
Significantly improved bone erosion and reduced tartrate resistant acidic phosphatase (TRAP) positive osteoclasts.
Did not affect humoral immune response.
Animal Model: 2×105 B16F10 or 1×105 RM-1 cells injected C57BL/6 wild-type (WT) mice (6-16 weeks)[2]
Dosage: 200 μg, combined with anti-CTLA4 mAb (200 μg) (HY-P99132)
Administration: Intraperitoneal injection (i.p.), on day -1, 0, and 2
Result: Significantly reduced the number of lung metastatic nodules, relying on NK cells and IFNγ.
Animal Model: 1×105 B16F10 or RM-1 cells injected C57BL/6 WT mice (6-16 weeks)[2]
Dosage: 200 μg, combined with anti-CTLA4 mAb (200 μg)
Administration: Intraperitoneal injection (i.p.), once every 2-4 days from day 3 to 12
Result: Significantly inhibited tumor growth, relying on FcγRIV and CD8+ T cells.
Animal Model: CS-exposed (for 24 weeks) induced COPD in female C57BL/6 mice (6-8 weeks)[3]
Dosage: 100 or 250 μg
Administration: Intraperitoneal injection (i.p.), twice weekly for 24 weeks
Result: Significantly improved muscle atrophy and restored muscle function.
Inhibited the expression of MuRF1/Trogin-1 and activation of NF-κB.
Gene ID

3057148  [NCBI]

Accession

AAC40113
Application

ELISA, FACS, Functional assay, Research in vivo
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Molecular Weight

150 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Anti-Mouse RANKL/CD254 Antibody (IK22/5)]
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation

Purity: 95.00%

SDS (251 KB)

COA (232 KB) SDS-PAGE (65 KB)

Inhibitory Antibodies User Guide (602 KB)
References
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Anti-Mouse RANKL/CD254 Antibody (IK22/5) Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse RANKL/CD254 Antibody (IK22/5)RANKL/RANKE1/E2/E3 EnzymeNF-κBE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseNuclear factor-κBNuclear factor-kappaBRANKLCD254MuRF1osteoporosismelanomaB16F10Inhibitorinhibitorinhibit

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Anti-Mouse RANKL/CD254 Antibody (IK22/5)
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