Anti-Mouse PSGL-1/CD162 Antibody (4RA10)

Cat. No.: HY-P990166 Purity: ≥95.0%: Data Sheet
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Anti-Mouse PSGL-1/CD162 Antibody (4RA10) is a rat-derived IgG1 κ type antibody inhibitor, targeting to mouse PSGL-1/CD162. Anti-Mouse PSGL-1/CD162 Antibody (4RA10) blocks PSGL signaling and inhibits leukocyte rolling. Anti-Mouse PSGL-1/CD162 Antibody (4RA10) can be used for the researches of inflammation, immunology and infection, such as pneumonia and lymphocytic choriomeningitis virus infection.

For research use only. We do not sell to patients.

Anti-Mouse PSGL-1/CD162 Antibody (4RA10) Chemical Structure

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Description

Isotype

Rat IgG1 kappa

Recommend Isotype Controls

Rat IgG1 kappa, Isotype Control

Species Reactivity

Mouse

IC₅₀ & Target

PSGL-1/CD162

In Vitro

Anti-Mouse PSGL-1/CD162 Antibody (4RA10) (5 μg/mL) inhibits d platelet-neutrophil aggregate formation of isolated WT neutrophils from pneumonia mice^[2].
Anti-Mouse PSGL-1/CD162 Antibody (4RA10) (5 μg/mL, 1 h) decreases platelet-Treg cell aggregate formation of isolated WT T reg cells^[2].
Anti-Mouse PSGL-1/CD162 Antibody (4RA10) (10 μg/mL, 20 mins) inhibits CD4 T cell-induced apoptosis of human Umbilical Vein Endothelial Cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Anti-Mouse PSGL-1/CD162 Antibody (4RA10) (5 μg/mouse, i.v., a single dose) inhibits P-selectin–independent rolling and E-selectin–mediated leukocyte rolling in TNF-treated cremaster microcirculation in mice^[1].
Anti-Mouse PSGL-1/CD162 Antibody (4RA10) (100 μg, i.p., twice on days 4, 6, 8, 10, and 12) shows anti-infection effect in lymphocytic choriomeningitis virus (LCMV) CI13-infected mice models^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	LCMV CI13-infected mice models^[1]
Dosage:	100 μg
Administration:	Intraperitoneally injection, twice on days 4, 6, 8, 10, and 12
Result:	Increased the frequency of cytokine-producing NP_(396-401)-specific and GP_(33-41)-specific CD8+ T cells. Decreased co-expression of the inhibitory receptors PD-1, LAG3, and TIM-3.

Gene ID

20345 [NCBI]

Accession

Q62170

Application

ELISA, FACS, Functional assay, Research in vivo

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Molecular Weight

150 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Anti-Mouse PSGL-1/CD162 Antibody (4RA10)]

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Purity: ≥95.0%

Select Batch:

Data Sheet (262 KB) SDS (251 KB)

COA (232 KB)

Inhibitory Antibodies User Guide (602 KB)

References

[1]. Zanardo RC, et al. A down-regulatable E-selectin ligand is functionally important for PSGL-1-independent leukocyte-endothelial cell interactions. Blood. 2004 Dec 1;104(12):3766-73. [Content Brief]

[2]. Rossaint J, et al. Platelets orchestrate the resolution of pulmonary inflammation in mice by T reg cell repositioning and macrophage education. J Exp Med. 2021 Jul 5;218(7):e20201353. [Content Brief]

[3]. Hope JL, et al. PSGL-1 attenuates early TCR signaling to suppress CD8+ T cell progenitor differentiation and elicit terminal CD8+ T cell exhaustion. Cell Rep. 2023 May 30;42(5):112436. [Content Brief]

[4]. Kitamura K, et al. P-Selectin Glycoprotein Ligand-1 (PSGL-1) Expressing CD4 T Cells Contribute Plaque Instability in Acute Coronary Syndrome. Circ J. 2018 Jul 25;82(8):2128-2135. [Content Brief]

Anti-Mouse PSGL-1/CD162 Antibody (4RA10) Related Classifications

Inflammation/Immunology Infection

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse PSGL-1/CD162 Antibody (4RA10)ApoptosisTransmembrane GlycoproteinArenavirusPSGL-1CD162LeukocytePneumoniaT cellsLymphocytic choriomeningitis virusInhibitorinhibitorinhibit

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