Anti-Mouse osteopontin/SPP1 Antibody (100D3)

Cat. No.: HY-P990795: Data Sheet
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Anti-Mouse osteopontin/SPP1 Antibody (100D3) is an anti-mouse osteopontin/SPP1 IgG2c monoclonal antibody. Anti-Mouse osteopontin/SPP1 Antibody (100D3) can reverse the inhibition of osteopontin (OPN) on T cells and enhance cytotoxic T lymphocyte (CTL) killing ability. Anti-Mouse osteopontin/SPP1 Antibody (100D3) can improve dentin density. Anti-Mouse osteopontin/SPP1 Antibody (100D3) can be used for researches on cancer and dental related conditions such as colon cancer. The recommend isotype control of Anti-Mouse osteopontin/SPP1 Antibody (100D3): Mouse IgG2c kappa, Isotype Control (HY-P99981).

For research use only. We do not sell to patients.

Anti-Mouse osteopontin/SPP1 Antibody (100D3) Chemical Structure

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Purity & Documentation
References
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Description

Isotype

Mouse IgG2c kappa

Recommend Isotype Controls

Mouse IgG2c kappa, Isotype Control

Species Reactivity

Mouse

IC₅₀ & Target

osteopontin/SPP1

In Vitro

Anti-Mouse osteopontin/SPP1 Antibody (100D3) (3 days) can reverse the inhibition of osteopontin (OPN) on T cell proliferation^[1].
Anti-Mouse osteopontin/SPP1 Antibody (100D3) (24 h) significantly enhances the killing efficiency of cytotoxic T lymphocyte (CTL) against CT26^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Anti-Mouse osteopontin/SPP1 Antibody (100D3) (200 μg, s.c., once every 3 days, for 14 days) significantly reduces lung metastatic nodules in Balb/c mice bearing CT26 tumors^[1].
Anti-Mouse osteopontin/SPP1 Antibody (100D3) (200 μg, s.c., twice a week, from day 7 to day 60) effectively improves dentin density in BALB/C mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	2×10⁵ CT26 cells injected Balb/c mice (2-3 months)^[1]
Dosage:	200 μg
Administration:	Subcutaneous injection (s.c.), once every 3 days, for 14 days
Result:	Significantly reduced the number of lung metastatic nodules.

Animal Model:	BALB/C mice (6 weeks)^[2]
Dosage:	200 μg
Administration:	Subcutaneous injection (s.c.), twice a week, from day 7 to day 60
Result:	Significantly increased the dentin density of the second and third molars. Had no significant improvement in alveolar bone volume, density, and thickness. Decreased serum FGF23 levels, but blood phosphorus levels did not return to normal.

Gene ID

20750 [NCBI]

Accession

P10923

Application

ELISA, FACS, Functional assay, Research in vivo

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Molecular Weight

150 kDa

SMILES

[Anti-Mouse osteopontin/SPP1 Antibody (100D3)]

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Inhibitory Antibodies User Guide (602 KB)

References

[1]. Klement JD, et al. Osteopontin Blockade Immunotherapy Increases Cytotoxic T Lymphocyte Lytic Activity and Suppresses Colon Tumor Progression. Cancers (Basel). 2021 Feb 28;13(5):1006. [Content Brief]

[2]. Mohamed FF, et al. Contributions of increased osteopontin and hypophosphatemia to dentoalveolar defects in osteomalacic Hyp mice. Bone. 2023 Nov;176:116886. [Content Brief]

Anti-Mouse osteopontin/SPP1 Antibody (100D3) Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse osteopontin/SPP1 Antibody (100D3)OsteopontinSecreted Phosphoprotein 1osteopontin/SPP1T cellOPNCTLtoothCT26Inhibitorinhibitorinhibit

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