Anti-Mouse Notch4 Antibody (HMN4-14)

Cat. No.: HY-P990177: Data Sheet
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Anti-Mouse Notch4 Antibody (HMN4-14) is an anti-mouse Notch4 IgG monoclonal antibody. Anti-Mouse Notch4 Antibody (HMN4-14) reduces inflammation by restoring the expression of bispecific proteins. Anti-Mouse Notch4 Antibody (HMN4-14) can inhibit Th2/Th17 cell differentiation and improve Treg cell function. Anti-Mouse Notch4 Antibody (HMN4-14) can be used for research on inflammation conditions such as viral pneumonia and airway inflammation.

For research use only. We do not sell to patients.

Anti-Mouse Notch4 Antibody (HMN4-14) Chemical Structure

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Biological Activity
Purity & Documentation
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Description

Isotype

Armenian hamster IgG

Recommend Isotype Controls

Polyclonal Armenian hamster IgG, Isotype Control

Species Reactivity

Mouse

IC₅₀ & Target

Notch4

In Vitro

Anti-Mouse Notch4 Antibody (HMN4-14) (10 μg/mL, 72 h) inhibits M1 polarization of macrophages and promotes M2 polarization^[1].
Anti-Mouse Notch4 Antibody (HMN4-14) (10 μg/mL, 72 h) significantly increases the expression of bispecific proteins in Treg cells^[1].
Anti-Mouse Notch4 Antibody (HMN4-14) (10 μg/mL, 72 h) significantly inhibits ultra fine particles (UFP) induced Th2/Th17 cell differentiation and reduce the transformation of Treg cells into Th-like cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Anti-Mouse Notch4 Antibody (HMN4-14) (10 μg, i.p., on day 4 and 7) relieves pulmonary inflammation in Foxp3^YFPcre mice with a H1N1 virus infection model^[1].
Anti-Mouse Notch4 Antibody (HMN4-14) (150 µg, i.p., once daily, for 3 days) significantly alleviates airway inflammation in Il4ra^R576 mice with an OVA (HY-P0286) induced airway inflammation model^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	40000 PFU H1N1 virus injected Foxp3^YFPcre mice^[1]
Dosage:	10 μg
Administration:	Intraperitoneal injection (i.p.), on day 4 and 7
Result:	Significantly reduced mortality rate. Reduced lung inflammation and cytokine storm.

Animal Model:	OVA (100 μg) induced Il4ra^R576 mice^[2]
Dosage:	150 µg
Administration:	Intraperitoneal injection (i.p.), once daily, for 3 days
Result:	Reduced inflammation score. Reduced eosinophil infiltration. Reduced Th2/Th17 cell responses and IgE levels.

Gene ID

18132 [NCBI]

Accession

P31695

Application

ELISA, FACS, Functional assay, Research in vivo

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Molecular Weight

150 kDa

SMILES

[Anti-Mouse Notch4 Antibody (HMN4-14)]

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Inhibitory Antibodies User Guide (602 KB)

References

[1]. Harb H, et al. Notch4 signaling limits regulatory T-cell-mediated tissue repair and promotes severe lung inflammation in viral infections. Immunity. 2021 Jun 8;54(6):1186-1199.e7. [Content Brief]

[2]. Xia M, et al. A Jagged 1-Notch 4 molecular switch mediates airway inflammation induced by ultrafine particles. J Allergy Clin Immunol. 2018 Oct;142(4):1243-1256.e17. [Content Brief]

Anti-Mouse Notch4 Antibody (HMN4-14) Related Classifications

Inflammation/Immunology Infection

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The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse Notch4 Antibody (HMN4-14)NotchInfluenza VirusNotch4TregTh2/Th17H1N1airway inflammationpneumoniaInhibitorinhibitorinhibit

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