Anti-Mouse NKG2A/C/E Antibody (20D5)

Cat. No.: HY-P990180 Purity: 95.00%: Data Sheet
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Anti-Mouse NKG2A/C/E Antibody (20D5) is an anti-mouse NKG2A/C/E IgG2a monoclonal antibody. Anti-Mouse NKG2A/C/E Antibody (20D5) can activate natural killer (NK) and CD8⁺ T cells. Anti-Mouse NKG2A/C/E Antibody (20D5) can exacerbate liver pathological damage by enhancing the activation of iNKT cells. Anti-Mouse NKG2A/C/E Antibody (20D5) can be used for research on cancer and immunology such as lymphoma.

For research use only. We do not sell to patients.

Anti-Mouse NKG2A/C/E Antibody (20D5) Chemical Structure

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Description

Isotype

Rat IgG2a kappa

Recommend Isotype Controls

Rat IgG2a kappa, Isotype Control

IC₅₀ & Target

NKG2A/C/E

In Vitro

Anti-Mouse NKG2A/C/E Antibody (20D5) (10 μg/mL, 4 h) enhances the perforin dependent cytotoxicity of liver mononuclear cells (MNCs), but has no effect on FasL/Fas dependent cytotoxicity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Anti-Mouse NKG2A/C/E Antibody (20D5) (300 μg, i.p., single dose, 2 days before Con A or α-GalCer injection) exacerbates Concanavallin A (Con A) (HY-NP096) or α-Galactosylceramide (α-GalCer) (HY-102022) induced hepatitis in C57BL/6 mice or DBA/2J mice^[1].
Anti-Mouse NKG2A/C/E Antibody (20D5) (200 μg, i.v., once every 3-4 days, for 3 times) significantly inhibits tumor growth conbinated with PD-L1 mAb in female C57BL/6J or BALB/c mice bearing A20 or RMA-Rae-1β tumors^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Con A (10 mg/kg, i.v.) induced C57BL/6 mice or α-GalCer induced (2 μg, i.p.) DBA/2J mice^[1]
Dosage:	300 μg
Administration:	Intraperitoneal injection (i.p.), single dose, 2 days before Con A or α-GalCer injection
Result:	Increased serum AST and ALT levels. Aggravated liver pathological damage. Increased serum IFNγ levels.

Animal Model:	5×10⁶ RMA-Rae-1β cells injected female C57BL/6J or 5×10⁶ A20 cells injected BALB/c mice^[2]
Dosage:	200 μg, conbinated with PD-L1 mAb (50 μg for BALB/c mice and 200 μg for C57BL/6J mice)
Administration:	Intravenous injection (i.v.), once every 3-4 days for 3 times
Result:	Significantly inhibited tumor growth (75% BALB/c mice survive), relying on NK and CD8⁺ T cells. Significantly inhibited tumor growth (45% C57BL/6J mouse survival) and induced long-lasting anti-tumor memory CD8⁺ T cells.

Gene ID

16641 [NCBI]

Accession

Q9WU31

Application

ELISA, FACS, Functional assay, Research in vivo

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Molecular Weight

150 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Anti-Mouse NKG2A/C/E Antibody (20D5)]

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Purity: 95.00%

Select Batch:

Data Sheet (262 KB) SDS (251 KB)

COA (232 KB)

Inhibitory Antibodies User Guide (602 KB)

References

[1]. Kawamura T, et al. NKG2A inhibits invariant NKT cell activation in hepatic injury. J Immunol. 2009 Jan 1;182(1):250-8. [Content Brief]

[2]. André P, et al. Anti-NKG2A mAb Is a Checkpoint Inhibitor that Promotes Anti-tumor Immunity by Unleashing Both T and NK Cells. Cell. 2018 Dec 13;175(7):1731-1743.e13. [Content Brief]

Anti-Mouse NKG2A/C/E Antibody (20D5) Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse NKG2A/C/E Antibody (20D5)C-type Lectin-like Receptors (CTLRs)NKG2A/C/ENKCD8immunologylymphomaRMA-Rae-1βA20Inhibitorinhibitorinhibit

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