2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease Apoptosis
  3. Interleukin Related Reactive Oxygen Species (ROS) Ferroptosis
  4. Anti-Mouse IL-1b Antibody (B122)

Anti-Mouse IL-1b Antibody (B122) 

Cat. No.: HY-P99139 Purity: 95.00%
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Anti-Mouse IL-1b Antibody (B122) is an anti-mouse IL-1b IgG monoclonal antibody. Anti-Mouse IL-1b Antibody (B122) enhances ferroptosis and increases levels of reactive oxygen species (ROS) combinated with Sulfasalazine (SAS) (HY-14655). Anti-Mouse IL-1b Antibody (B122) can reduce monocyte infiltration and alleviate T cell exhaustion by blocking IL-1β signaling. Anti-Mouse IL-1b Antibody (B122) can be used for research on cancer and cardiovascular conditions such as oral squamous cell carcinoma (OSCC), glioblastoma (GBM) and heart failure.

For research use only. We do not sell to patients.

Anti-Mouse IL-1b Antibody (B122) Chemical Structure

Anti-Mouse IL-1b Antibody (B122) Chemical Structure

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Description

Anti-Mouse IL-1b Antibody (B122) is an anti-mouse IL-1b IgG monoclonal antibody. Anti-Mouse IL-1b Antibody (B122) enhances ferroptosis and increases levels of reactive oxygen species (ROS) combinated with Sulfasalazine (SAS) (HY-14655). Anti-Mouse IL-1b Antibody (B122) can reduce monocyte infiltration and alleviate T cell exhaustion by blocking IL-1β signaling. Anti-Mouse IL-1b Antibody (B122) can be used for research on cancer and cardiovascular conditions such as oral squamous cell carcinoma (OSCC), glioblastoma (GBM) and heart failure[1][2][3].

Isotype

Armenian hamster IgG
Recommend Isotype Controls
IC50 & Target[1][2][3]

IL-1

 

In Vitro

Anti-Mouse IL-1b Antibody (B122) (50 pM, 6-72 h) significantly inhibits the proliferation of various tumor cells combinated with Sulfasalazine (SAS), including SCC1, A375 and MDA-MB231 cells[1].
Anti-Mouse IL-1b Antibody (B122) (50 pM, 24 h) reduces the level of IL-1β and enhances the expression of ferroptosis markers combinated with SAS in SCC1 cells[1].
Anti-Mouse IL-1b Antibody (B122) (50 pM, 24 h) can increase the level of reactive oxygen species and decrease the level of GSH combinated with SAS in SCC1 cells[1].
Anti-Mouse IL-1b Antibody (B122) (50 pM) reverses T cell exhaustion and improves T cell function combinated with 40 % SAS conditioned medium[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Mouse IL-1b Antibody (B122) Related Antibodies

Cell Viability Assay[1]

Cell Line: SCC1, A375 and MDA-MB231 cells
Concentration: 50 pM, combinated with SAS (800 μM)
Incubation Time: 6, 12, 24, 48 and 72 h
Result: Significantly reduced the cell viability of three types of cells.

RT-PCR[1]

Cell Line: SCC1 cells
Concentration: 50 pM, combinated with SAS (800 μM)
Incubation Time: 24 h
Result: Reduced the level of PTSG2 and IL-1β and increased the level of SLC7A11.

Western Blot Analysis[1]

Cell Line: SCC1 cells
Concentration: 50 pM, combinated with SAS (800 μM)
Incubation Time: 24 h
Result: Reduced the level of PTSG2 and IL-1β and increased the level of SLC7A11.
In Vivo

Anti-Mouse IL-1b Antibody (B122) (100 μg/kg, intratumoral injection, once every 3 days, for 4 weeks) significantly inhibits tumor development combinated with SAS in male Sprague-Dawley (SD) rats bearing the oral squamous cell carcinoma (OSCC)[1].
Anti-Mouse IL-1b Antibody (B122) (1 mg, intratumoral injection, once daily, until the endpoint) significantly prolongs the survival period in Ntv-a mice with PDGFB overexpression and p53 silencing[2].
Anti-Mouse IL-1b Antibody (B122) (100 μg, i.p., twice weekly, for 2 weeks) has a protective effect on right ventricular function and pulmonary vascular remodeling in C57BL/6J mice with a heart failure with preserved ejection fraction (HFpEF) model[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Nitrochin (HY-33354) (20 ppm) induced male Sprague-Dawley (SD) rats (4 weeks)[1]
Dosage: 100 μg/kg, combinated with SAS (30 mg/kg)
Administration: Intratumoral injection, once every 3 days for 4 weeks
Result: Caused only 16.6 % of cases to develop into mild invasive cancer, while 66.6 % of the control group had moderate invasive cancer.
Significantly inhibited tumor development with the lowest HE score.
Reduced the expression of SLC7A11 and IL-1β and increased the expression of PTGS2.
Reduced Ki67+ cells.
Animal Model: Ntv-a mice with a PDGFB glioblastoma (GBM) model(8-12 weeks)[2]
Dosage: 1 mg
Administration: Intratumoral injection, once daily, until the endpoint
Result: Significantly prolonged the survival of PDGFB GBM mice (median survival extended from 40 days to 47 days).
Reduced tumor associated macrophage (TAMs) infiltration.
Reduced the expression of T cell exhaustion markers (such as PD-1).
Animal Model: L-NAME (HY-18729) (0.5 g/L) and high-fat diet (60 % lipid) administrated C57BL/6J mice (8 weeks)[3]
Dosage: 100 μg
Administration: Intraperitoneal injection (i.p.), twice weekly, for 2 weeks
Result: Significantly reduced the level of IL-1β in lung tissue.
Reduced elevated right ventricular systolic pressure and pulmonary small vessel muscularization.
Had no significant effect on weight changes and right ventricular end diastolic pressure.
Gene ID

16176  [NCBI]

Accession
Application

ELISA, FACS, Functional assay, Research in vivo
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Molecular Weight

150 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Anti-Mouse IL-1b Antibody (B122)]
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Anti-Mouse IL-1b Antibody (B122) Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse IL-1b Antibody (B122)Interleukin RelatedReactive Oxygen Species (ROS)FerroptosisILIL-1bferroptosisROSGBMOSCCheart failureInhibitorinhibitorinhibit

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