Anti-Mouse ICOS Antibody (7E.17G9)

Cat. No.: HY-P990237: Data Sheet
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Anti-Mouse ICOS Antibody (7E.17G9) is an anti-mouse ICOS IgG2b monoclonal antibody. Anti-Mouse ICOS Antibody (7E.17G9) can upregulate ICOS expression and enhance T cell function. Anti-Mouse ICOS Antibody (7E.17G9) can significantly inhibit the immune regulatory function of dendritic cells (DCs). Anti-Mouse ICOS Antibody (7E.17G9) can eliminate transplant tolerance. Anti-Mouse ICOS Antibody (7E.17G9) can be used for researches on inflammation conditions, cancer and xenotransplantation such as nephritis and pancreatic cancer.

For research use only. We do not sell to patients.

Anti-Mouse ICOS Antibody (7E.17G9) Chemical Structure

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Description

Isotype

Rat IgG2b kappa

Recommend Isotype Controls

Rat IgG2b kappa, Isotype Control

Species Reactivity

Mouse

IC₅₀ & Target

ICOS

In Vitro

Anti-Mouse ICOS Antibody (7E.17G9) (10 μg/mL, 48 h) significantly inhibits IL-10⁺ Tregs induced by MPO/BAY dendritic cells (DCs) and suppresses Tregs activation^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Anti-Mouse ICOS Antibody (7E.17G9) (250-375 μg, i.p., on day 0, 2, 4 and 6) significantly reduces transplant tolerance in PD-1 Tg B6 mice with an ectopic abdominal heart transplantation^[1].
Anti-Mouse ICOS Antibody (7E.17G9) (0.2 mg, i.p., once every other day, from day 13 to day 25) significantly inhibits the induction of IL-10⁺ Tregs in C57BL/6 mice with a MPO immune model^[2].
Anti-Mouse ICOS Antibody (7E.17G9) (100 μg, i.p., on day 4, 7 and 10) significantly prolongs the survival period of C57BL/6 mice bearing KPC tumors combined with RIP1i^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	PD-1 Tg B6 mice (8-12 weeks)^[1]
Dosage:	375 μg on day 0, 250 μg on day 2, 4 and 6
Administration:	Intraperitoneal injection (i.p.), on day 0, 2, 4 and 6
Result:	Significantly shortened the survival time of transplants. Slightly increased the production of IFN-γ, but significantly reduced the production of IL-10. Reduced IL-10 production and LAP expression in Treg cells.

Animal Model:	MPO (20 μg) and CFA (HY-153808) injected C57BL/6 mice (8-12 weeks)^[2]
Dosage:	0.2 mg
Administration:	Intraperitoneal injection (i.p.), once every other day, from day 13 to day 25
Result:	Significantly inhibited the induction of IL-10⁺ Tregs by MPO/BAY DCs. Inhibited the upregulation of Foxp3, CTLA4 and TNFR2.

Animal Model:	1×10⁵ KPC cells injected C57BL/6 mice (8-10 weeks)^[3]
Dosage:	100 μg, combined with RIP1i (100 mg/kg)
Administration:	Intraperitoneal injection (i.p.), on day 4, 7 and 10
Result:	Upregulated ICOS expression and enhances T cell function. Significantly prolonged the survival period of mice.

Gene ID

54167 [NCBI]

Accession

Q9WVS0

Application

ELISA, FACS, Functional assay, Research in vivo

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Molecular Weight

150 kDa

SMILES

[Anti-Mouse ICOS Antibody (7E.17G9)]

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Inhibitory Antibodies User Guide (602 KB)

References

[1]. Borges TJ, et al. Overexpression of PD-1 on T cells promotes tolerance in cardiac transplantation via ICOS-dependent mechanisms. JCI Insight. 2021 Dec 22;6(24):e142909. [Content Brief]

[2]. Odobasic D, et al. Tolerogenic Dendritic Cells Attenuate Experimental Autoimmune Antimyeloperoxidase Glomerulonephritis. J Am Soc Nephrol. 2019 Nov;30(11):2140-2157. [Content Brief]

[3]. Wang W, et al. RIP1 Kinase Drives Macrophage-Mediated Adaptive Immune Tolerance in Pancreatic Cancer. Cancer Cell. 2018 Nov 12;34(5):757-774.e7. [Content Brief]

Anti-Mouse ICOS Antibody (7E.17G9) Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse ICOS Antibody (7E.17G9)CD28ICOST celltransplantspancreatic cancerKPCDCsnephritisInhibitorinhibitorinhibit

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