2. Apoptosis NF-κB Immunology/Inflammation
  3. TNF Receptor NF-κB Interleukin Related
  4. Anti-Mouse GITR Antibody (DTA-1)

Anti-Mouse GITR Antibody (DTA-1) 

Cat. No.: HY-P990124 Purity: 98.47%
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Anti-Mouse GITR Antibody (DTA-1) is a rat derived IgG2b monoclonal agonist antibody targeting mouse GITR. Anti-Mouse GITR Antibody (DTA-1) can activate T cells and B cells to enhance immune response. Anti-Mouse GITR Antibody (DTA-1) selectively enhances Th2 cell effector function through GITR signaling. Anti-Mouse GITR Antibody (DTA-1) may exacerbate allergic reactions. Anti-Mouse GITR Antibody (DTA-1) can be used for researches on cancer and allergic conditions such as colon cancer and allergic asthma. The recommed isotype control of Anti-Mouse GITR Antibody (DTA-1) is Rat IgG2b kappa, Isotype Control (HY-P990682).

For research use only. We do not sell to patients.

Anti-Mouse GITR Antibody (DTA-1)

Anti-Mouse GITR Antibody (DTA-1) Chemical Structure

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Anti-Mouse GITR Antibody (DTA-1) Related Antibodies

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Description

Anti-Mouse GITR Antibody (DTA-1) is a rat derived IgG2b monoclonal agonist antibody targeting mouse GITR. Anti-Mouse GITR Antibody (DTA-1) can activate T cells and B cells to enhance immune response. Anti-Mouse GITR Antibody (DTA-1) selectively enhances Th2 cell effector function through GITR signaling. Anti-Mouse GITR Antibody (DTA-1) may exacerbate allergic reactions. Anti-Mouse GITR Antibody (DTA-1) can be used for researches on cancer and allergic conditions such as colon cancer and allergic asthma. The recommed isotype control of Anti-Mouse GITR Antibody (DTA-1) is Rat IgG2b kappa, Isotype Control (HY-P990682)[1][2][3].

Isotype

Rat IgG2b
Species Reactivity

Mouse
IC50 & Target[1][3]

NF-κB

 

IL-4

 

In Vitro

Anti-Mouse GITR Antibody (DTA-1) (0.0001-100 μg/mL) can be combined with a muGITR-HEK293 transfectant cell line with the EC50 of 0.14 μg/mL[1].
Anti-Mouse GITR Antibody (DTA-1) (0.0001-100 μg/mL, 72-120 h) can enhance TCR triggered T cell proliferation and survival with the EC50 of 0.02 μg/mL[1].
Anti-Mouse GITR Antibody (DTA-1) can activate NF-κB signaling with the EC50 of 0.08 μg/mL[1].
Anti-Mouse GITR Antibody (DTA-1) (5 days) significantly increases the expression of CD86 on the surface of B cells and induces differentiation of CD138+ plasma cells[2].
Anti-Mouse GITR Antibody (DTA-1) (10 μg/mL, 4 days) significantly increases IL-4 secretion in Th2 cells, but has no effect on IFN-γ secretion in Th1 cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Mouse GITR Antibody (DTA-1) Related Antibodies

ELISA Assay[3]

Cell Line: CD4+CD25- T cells
Concentration: 10 μg/mL
Incubation Time: 4 days
Result: Significant increased the level of IL-4.
In Vivo

Anti-Mouse GITR Antibody (DTA-1) (3.0 mg/kg, i.p., on day 7, 9, and 11) significantly inhibits tumor growth in wild type C57BL/6 mice bearing MC-38 tumors[1].
Anti-Mouse GITR Antibody (DTA-1) (3.0 mg/kg, i.p., on day 0, 4 and 11 or on day 0, 4, 11 and 18) causes severe allergic reactions in wild type C57BL/6 mice (Healthy or bearing MC-38 tumors)[1].
Anti-Mouse GITR Antibody (DTA-1) (500 μg, i.p., on day 7 and 14) significantly inhibits tumor growth in female Balb/c and CD40−/− mice bearing CT26 or EMT6 tumors[2].
Anti-Mouse GITR Antibody (DTA-1) (1 mg, i.p., single dose, 1 h before the first OVA challenge) exacerbates airway hyperresponsiveness in asthma by selectively enhancing Th2 effector function in male BALB/c mice with a OVA (HY-P0286) induced allergic asthma model[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 1×106 MC-38 cells injected wild type C57BL/6 mice (5-6 weeks)[1]
Dosage: 3.0 mg/kg
Administration: Intraperitoneal injection (i.p.), on day 7, 9, and 11
Result: Significantly inhibited the increase in tumor volume.
Increased the proportion of CD8+ T cells and regulatory T cells (Treg) within the tumor.
Animal Model: Wild type C57BL/6 mice (Healthy or bearing MC-38 tumors) (5-6 weeks)[1]
Dosage: 3.0 mg/kg for healthy mice, 0.1, 0.3, 1.0, 3.0, and 10.0 mg/kg for mice bearing MC-38 mice
Administration: Intraperitoneal injection (i.p.), on day 0, 4, and 11 or on day 0, 4, 11 and 18
Result: Caused a decrease in body temperature and behavioral abnormalities in mice.
Increased IL-4 and IgE levels.
Animal Model: 3×105 CT26 or 1×105 EMT6 cells injected female Balb/c and 3×105 CT26 cells injected CD40−/− mice[2]
Dosage: 500 μg
Administration: Intraperitoneal injection (i.p.), on day 7 and 14
Result: Significantly regressed 85% of the tumor in Balb/c mice and 50% of the tumor in CD40−/− mice. Increased the number of CD4+ and CD8+ T cells.
Increased the level of IFN-γ, IL-2, IL-5, and IL-10.
Increased serum IgG1 and IgG2a levels by 20 fold and 7.5 fold and generated tumor reactive IgG1 antibodies.
Animal Model: OVA (10 μg, i.p., on day 0 and 7; 10 mg/ml, inhalation, on day 21, 24 and 27) induced male BALB/c mice[3]
Dosage: 1 mg
Administration: Intraperitoneal injection (i.p.), single dose, 1 h before the first OVA challenge
Result: Significantly enhanced sensitivity to acetylcholine.
Significantly increased OVA specific IgE levels.
Had no significant change in the number of eosinophils and lymphocyte infiltration.
Significantly increased the levels of IL-5, IL-10 and IL-13.
Gene ID

21936  [NCBI]

Accession

O35714-1
Application

ELISA, FACS, Functional assay, Research in vivo
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Molecular Weight

150 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Anti-Mouse GITR Antibody (DTA-1)]
Shipping

Shipping with dry ice.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Anti-Mouse GITR Antibody (DTA-1) Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse GITR Antibody (DTA-1)TNF ReceptorNF-κBInterleukin RelatedTumor Necrosis Factor ReceptorTNFRNuclear factor-κBNuclear factor-kappaBILGITRT cellsB cellscolon cancerallergic asthmaMC-38CT26Inhibitorinhibitorinhibit

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