2. Immunology/Inflammation
  3. CTLA-4
  4. Anti-Mouse CTLA-4 Antibody (9D9)

Anti-Mouse CTLA-4 Antibody (9D9) 

Cat. No.: HY-P99132 Purity: 95.00%
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Anti-Mouse CTLA-4 Antibody (9D9) is an anti-mouse CTLA-4 IgG2b monoclonal antibody. Anti-Mouse CTLA-4 Antibody (9D9) can bind to CTLA-4 and block its binding to B7. Anti-Mouse CTLA-4 Antibody (9D9) enhances T cell function by regulating the ratio of CD8+ T cells to regulatory T cells (Tregs). Anti-Mouse CTLA-4 Antibody (9D9) can be used for research on cancer such as colon cancer and melanoma.

For research use only. We do not sell to patients.

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  • References

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Description

Anti-Mouse CTLA-4 Antibody (9D9) is an anti-mouse CTLA-4 IgG2b monoclonal antibody. Anti-Mouse CTLA-4 Antibody (9D9) can bind to CTLA-4 and block its binding to B7. Anti-Mouse CTLA-4 Antibody (9D9) enhances T cell function by regulating the ratio of CD8+ T cells to regulatory T cells (Tregs). Anti-Mouse CTLA-4 Antibody (9D9) can be used for research on cancer such as colon cancer and melanoma[1][2].

Isotype

Mouse IgG2b kappa
Recommend Isotype Controls
IC50 & Target

CTLA-4
In Vitro

Anti-Mouse CTLA-4 Antibody (9D9) (0.1-1000 nM, 2 h) has high binding strength with CTLA-4WT (KD = 15.4 nM) and CTLA-4del36 (KD = 69.6 nM) in HEK293-F cells[1].
Anti-Mouse CTLA-4 Antibody (9D9) (0.01-1000 nM, 1 h) significantly blocks the binding of B7 to CTLA-4 in HEK293-F cells (IC50 = 23.5 nM for ELISA, IC50 = 24.4 nM for cell surface)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Mouse CTLA-4 Antibody (9D9) Related Antibodies
In Vivo

Anti-Mouse CTLA-4 Antibody (9D9) (200 μg, i.p. or i.t., on day 7, 10 and 14) has significant anti-tumor activity and increases CD4+/CD8+ T cell activation in female C57BL/6 mice bearing MC38 tumors[1].
Anti-Mouse CTLA-4 Antibody (9D9) (100 μg/dose, i.p., on day 3, 6 and 9) has significant anti-tumor activity and prolongs survival in male C57BL/6 mice bearing B16-F10 tumors[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 1 M MC38 cells injected female C57BL/6 mice (6-8 weeks)[1]
Dosage: 200 μg
Administration: Intraperitoneal injection (i.p.) or intratumoral injection (i.t.), on day 7, 10 and 14
Result: Achieved a cure rate of 47 % (i.t.) or 33 % (i.p.).
Exhausted intratumoral Tregs and activates lymph node T cells.
Animal Model: 2.5×104 B16-F10 cells injected male C57BL/6 mice (6 weeks)[2]
Dosage: 100 μg/dose, combined with MGN1703
Administration: Intraperitoneal injection (i.p.), on day 3, 6 and 9
Result: Cured 50% of mice and prolongs survival time.
Gene ID

12477  [NCBI]

Accession
Application

ELISA, FACS, Functional assay, Research in vivo
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Molecular Weight

150 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Anti-Mouse CTLA-4 Antibody (9D9)]
Shipping

Shipping with dry ice.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
  • Immobilized CTLA-4 Protein, Mouse (HEK293, His, HY-P71991) can bind Anti-Mouse CTLA-4 Antibody (9D9). The EC50 for this effect is 1.979 ng/mL.
Purity & Documentation

Purity: ≥98.0%

SDS (251 KB)

COA (292 KB)

Inhibitory Antibodies User Guide (602 KB)
References
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Anti-Mouse CTLA-4 Antibody (9D9) Related Classifications

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse CTLA-4 Antibody (9D9)CTLA-4Cytotoxic T-lymphocyte-associated protein 4CD152colon cancermelanomaTregsInhibitorinhibitorinhibit

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