2. Immunology/Inflammation
  3. CD47
  4. Anti-Mouse CD47/IAP Antibody (MIAP301)

Anti-Mouse CD47/IAP Antibody (MIAP301) 

Cat. No.: HY-P990131 Purity: ≥95.0%
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Anti-Mouse CD47/IAP Antibody (MIAP301) is an anti-mouse CD47/IAP IgG2a monoclonal antibody. Anti-Mouse CD47/IAP Antibody (MIAP301) can effectively block CD47 signaling and enhance macrophage phagocytic function. Anti-Mouse CD47/IAP Antibody (MIAP301) can increase the infiltration of immune cells. Anti-Mouse CD47/IAP Antibody (MIAP301) restores the phagocytic function of myeloid cells and alleviate B cell inhibition. Anti-Mouse CD47/IAP Antibody (MIAP301) may interfere with wound healing. Anti-Mouse CD47/IAP Antibody (MIAP301) can be used for researches on cancer, inflammation and infection conditions such as melanoma, intestinal mucosal repair and sepsis.

For research use only. We do not sell to patients.

Anti-Mouse CD47/IAP Antibody (MIAP301)

Anti-Mouse CD47/IAP Antibody (MIAP301) Chemical Structure

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Description

Anti-Mouse CD47/IAP Antibody (MIAP301) is an anti-mouse CD47/IAP IgG2a monoclonal antibody. Anti-Mouse CD47/IAP Antibody (MIAP301) can effectively block CD47 signaling and enhance macrophage phagocytic function. Anti-Mouse CD47/IAP Antibody (MIAP301) can increase the infiltration of immune cells. Anti-Mouse CD47/IAP Antibody (MIAP301) restores the phagocytic function of myeloid cells and alleviate B cell inhibition. Anti-Mouse CD47/IAP Antibody (MIAP301) may interfere with wound healing. Anti-Mouse CD47/IAP Antibody (MIAP301) can be used for researches on cancer, inflammation and infection conditions such as melanoma, intestinal mucosal repair and sepsis[1][2][3].

Isotype

Rat IgG2a kappa
Recommend Isotype Controls
Species Reactivity

Mouse
IC50 & Target

CD47/IAP
In Vitro

Anti-Mouse CD47/IAP Antibody (MIAP301) (10 μg/mL, 24 h) significantly inhibits epithelial cell migration and wound closure[1].
Anti-Mouse CD47/IAP Antibody (MIAP301) (0-4 h) inhibits the β1 integrin-FAK-SRc-p130Cas signaling pathway and reduces the level of p-SrcY 416, p-FAKY 397, p-FAKY Y861, p-p130Cas Y410 and β1 integrin[1].
Anti-Mouse CD47/IAP Antibody (MIAP301) (0-100 μg/mL, 1 h) effectively blocks CD47 and saturates its epitope in SM1 cells[2].
Anti-Mouse CD47/IAP Antibody (MIAP301) (25 μg/mL, 2-4 h) significantly enhances the phagocytic activity of macrophages towards SM1 cells[2].
Anti-Mouse CD47/IAP Antibody (MIAP301) (8 h) significantly restores the phagocytic ability of myeloid cells towards Escherichia coli[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Mouse CD47/IAP Antibody (MIAP301) Related Antibodies

Cell Migration Assay [1]

Cell Line: Epithelial cells
Concentration: 10 μg/mL
Incubation Time: 24 h
Result: Significant delayed in cell migration and wound closure.
In Vivo

Anti-Mouse CD47/IAP Antibody (MIAP301) (400 μg, i.p., single dose, 24 hours after the wound) significantly inhibits mucosal wound healing in male C57Bl/6J mice with a biopsy-based mucosal wound model[1].
Anti-Mouse CD47/IAP Antibody (MIAP301) (100 μg, intratumorally injection, once every three days, until the end of the study) significantly inhibits tumor growth and increases immune cell infiltration in the tumor microenvironment combined with Nexturastat A (HY-16699) in female C57BL/6 mice bearing SM1 or B16F10 tumors[2].
Anti-Mouse CD47/IAP Antibody (MIAP301) (2.5 mg/kg, i.v., single dose, 1 or 8 hours after cecal ligation and puncture (CLP) surgery) significantly reduces the number of bacteria in blood and organs and improves survival rates in Balb/c or C57/BL6 mice with a Klebsiella pneumonia-induced sepsis model[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57Bl/6J mice with a biopsy-based mucosal wound (8-12 weeks)[1]
Dosage: 400 μg
Administration: Intraperitoneal injection (i.p.), single dose, 24 hours after the wound
Result: Caused delayed wound closure.
Animal Model: 0.75×106 SM1 or B16F10 cells injected female C57BL/6J mice (4-6 weeks)[2]
Dosage: 100 μg, combined with Nexturastat A (20 mg/kg, i.p., once every other day)
Administration: Intraperitoneal injection (i.p.), once every three days until the end of the study
Result: Significantly inhibited tumor volume.
Significantly increased infiltration of macrophages and natural killer (NK) cells in SM1 models.
Animal Model: Balb/c or C57/BL6 mice (8-10 weeks, 20-25 g) with a Klebsiella pneumonia-induced sepsis model[3]
Dosage: 2.5 mg/kg
Administration: Intravenous injection (i.v.), single dose, 1 or 8 hours after CLP surgery
Result: Significantly reduced bacterial load in blood and organs.
Improved survival rate (from 30% to 80%).
Reduced pathological damage to the lungs and kidneys.
Reduced the levels of inflammatory factors (TNF-α, IL-6, IL-1β, MCP-1).
Gene ID

16423  [NCBI]

Accession

Q61735-1
Application

ELISA, FACS, Functional assay, Research in vivo
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Molecular Weight

150 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Anti-Mouse CD47/IAP Antibody (MIAP301)]
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation

Purity: ≥95.0%

SDS (251 KB)

COA (232 KB)

Inhibitory Antibodies User Guide (602 KB)
References
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Anti-Mouse CD47/IAP Antibody (MIAP301) Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse CD47/IAP Antibody (MIAP301)CD47Cluster of Differentiation 47MER6OA3CD47/IAPmacrophageepithelial cellsSM1B16F10immune cellssepsisInhibitorinhibitorinhibit

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