Anti-Mouse CD3ε Antibody (145-2C11)

Cat. No.: HY-P990802 Purity: 99.30%: Data Sheet
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Anti-Mouse CD3ε Antibody (145-2C11) is an orally active anti-mouse CD3ε IgG1 monoclonal antibody. Anti-Mouse CD3ε Antibody (145-2C11) can inhibit the proliferation of effector T cells and improve the function of regulatory T cells (Tregs). Anti-Mouse CD3ε Antibody (145-2C11) relieves inflammatory response by inhibiting the production of pro-inflammatory cytokines and increasing the secretion of anti-inflammatory factors. Anti-Mouse CD3ε Antibody (145-2C11) can be used for researches on metabolic and immune system conditions such as diabetes and lupus. The recommend isotype control of Anti-Mouse CD3ε Antibody (145-2C11): Armenian hamster IgG, Isotype Control (HY-P990305).

For research use only. We do not sell to patients.

Anti-Mouse CD3ε Antibody (145-2C11) Chemical Structure

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Description

Isotype

Armenian hamster IgG

Recommend Isotype Controls

Polyclonal Armenian hamster IgG, Isotype Control

Species Reactivity

Mouse

IC₅₀ & Target

CD3ε

In Vitro

Anti-Mouse CD3ε Antibody (145-2C11) (0.04-1 μg/mL, 72 h) significantly reduces the proliferation of splenic cells^[1].
Anti-Mouse CD3ε Antibody (145-2C11) (1 μg/mL, 48 h) inhibits the production of pro-inflammatory cytokines and increases the production of anti-inflammatory cytokines in splenic cells and pancreatic lymph node cells (pLNs)^[1].
Anti-Mouse CD3ε Antibody (145-2C11) (5 μg/mL, 2 h) induces macrophages to engulf T cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	Splenic cells
Concentration:	0.04, 0.02 and 1 μg/mL
Incubation Time:	72 h
Result:	Significantly inhibited the proliferation of splenic cells at 1 μg/mL.

ELISA Assay^[1]

Cell Line:	Splenic cells and pLNs
Concentration:	1 μg/mL
Incubation Time:	48 h
Result:	Increased the levels of TGF-β and IL-10 and decreased the levels of IFN-γ and IL-17.

In Vivo

Anti-Mouse CD3ε Antibody (145-2C11) (10 μg, p.o. or nasal administration, once daily for the first 5 days and then once per week until 20 weeks of age) significantly reduces the incidence rate of diabetes in female NOD mice^[1].
Anti-Mouse CD3ε Antibody (145-2C11) (50 μg, p.o., once daily, for 5 days) reduces the pathogenicity of effector T cells and improves T cell function in female NOD-huCD3ε mice^[1].
Anti-Mouse CD3ε Antibody (145-2C11) (3 mg/kg, i.p., once weekly, for 20 weeks) prolongs the survival period in female BWF1 mice with a lupus model^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female NOD mice (6 weeks)^[1]
Dosage:	10 μg
Administration:	Oral administration (p.o.) or nasal administration, once daily for the first 5 days and then once per week until 20 weeks of age
Result:	Significantly reduced the incidence rate of diabetes to 50 %. Reduced pancreatic islet infiltration and inflammation.

Animal Model:	Female NOD-huCD3ε mice (12 weeks)^[1]
Dosage:	50 μg
Administration:	Oral administration (p.o.), once daily, for 5 days
Result:	Reduced the pathogenicity of effector T cells and enhanced the function of regulatory T cells. Reduced the incidence rate of diabetes.

Animal Model:	Female BWF1 mice (20 weeks) with a lupus model^[2]
Dosage:	3 mg/kg
Administration:	Intraperitoneal injection (i.p.), once weekly for 20 weeks
Result:	Significantly reduced proteinuria in mice. Extended the survival period of mice.

Gene ID

12501 [NCBI]

Accession

P22646

Application

ELISA, FACS, Functional assay, Research in vivo

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Molecular Weight

150 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Anti-Mouse CD3e Antibody (145-2C11)]

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Purity: 99.30%

Select Batch:

Data Sheet (263 KB) SDS (251 KB)

COA (233 KB) SDS-PAGE (64 KB) SEC-HPLC (109 KB)

Inhibitory Antibodies User Guide (602 KB)

References

[1]. Kuhn C, et al. Mucosal administration of CD3-specific monoclonal antibody inhibits diabetes in NOD mice and in a preclinical mouse model transgenic for the CD3 epsilon chain. J Autoimmun. 2017 Jan;76:115-122. [Content Brief]

[2]. Morita M, et al. Reduction of Cell Surface T-Cell Receptor by Non-Mitogenic CD3 Antibody to Mitigate Murine Lupus. Front Immunol. 2022 Mar 28;13:855812. [Content Brief]

Anti-Mouse CD3ε Antibody (145-2C11) Related Classifications

Metabolic Disease Inflammation/Immunology

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse CD3ε Antibody (145-2C11)CD3Cluster of differentiation 3CD3εT celldiabeteslupusInhibitorinhibitorinhibit

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