2. Immunology/Inflammation
  3. CD3
  4. Anti-Mouse CD3 Antibody (17A2)

Anti-Mouse CD3 Antibody (17A2) 

Cat. No.: HY-P99124 Purity: 98.56%
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Anti-Mouse CD3 Antibody (17A2) is an anti-mouse CD3 IgG2b antibody inhibitor derived from the host Rat. Anti-Mouse CD3 Antibody (17A2) enhances T-cell proliferation and activation and stimulate IFN-γ or IL-17 production in splenic T cells. Anti-Mouse CD3 Antibody (17A2) significantly suppresses mortality in Graft-versus-host disease (GVHD) mice models.

For research use only. We do not sell to patients.

Anti-Mouse CD3 Antibody (17A2) Chemical Structure

Anti-Mouse CD3 Antibody (17A2) Chemical Structure

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Description

Anti-Mouse CD3 Antibody (17A2) is an anti-mouse CD3 IgG2b antibody inhibitor derived from the host Rat. Anti-Mouse CD3 Antibody (17A2) enhances T-cell proliferation and activation and stimulate IFN-γ or IL-17 production in splenic T cells. Anti-Mouse CD3 Antibody (17A2) significantly suppresses mortality in Graft-versus-host disease (GVHD) mice models[1][2].

Isotype

Rat IgG2b kappa
Recommend Isotype Controls
In Vitro

Anti-Mouse CD3 Antibody (17A2) (1-1000 ng/mL, 96 h) enhances T-cell proliferation and activation and stimulate IFN-γ or IL-17 production in splenic pan-T cells[1].
Anti-Mouse CD3 Antibody (17A2) (1 μg/mL, 48 h) significantly elevates mRNA levels for these Th1 and IL-17 genes in splenic T cells[1].
Anti-Mouse CD3 Antibody (17A2) (48 h) significantly enhances IRBP (interphotoreceptor retinoid-binding protein)-stimulated production of IFN-γ in ocular cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Mouse CD3 Antibody (17A2) Related Antibodies

RT-PCR[1]

Cell Line: Splenic T cells
Concentration: 1 μg/mL
Incubation Time: 48 h
Result: Increased the levels of IL-1α, IFN-γ, T-bet and IL-17 and decreased the levels of IL-10 and Foxp3.
In Vivo

Anti-Mouse CD3 Antibody (17A2) (100 or 400 μg, i.v., a single dose) depletes T cells in mice[2].
Anti-Mouse CD3 Antibody (17A2) (200 or 400 μg, i.v., 20 h after irradiation and 4 h before transplantation) significantly suppresses mortality in Graft-versus-host disease (GVHD) mice models [2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice[2]
Dosage: 100 and 400 μg
Administration: Intravenously injection
Result: Significantly reduced T cells.
Animal Model: Graft-versus-host disease (GVHD) mice models[2]
Dosage: 200 and 400 μg
Administration: Intravenously injection, 20 h after irradiation and 4 h before spleen cells and bone marrow cells transplantation
Result: Suppressed GVHD mortality by about 50% on day 100 posttransplantation and 20% to 40% on day 200.
Prolonged antibody treatment by further injections of 200 μg on days 3,6, and 9 after transplantation.
led to long-term survivors with 90% to 97% chimerism on days 50 and 100.
Reduced CD4+ cells to about 50% and CD8+ cells to 10% after 50 days.
Application

ELISA, FACS, Functional assay, Research in vivo
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Molecular Weight

150 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Anti-Mouse CD3 Antibody (17A2)]
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation

Purity: 98.56%

SDS (251 KB)

COA (232 KB) SEC-HPLC (124 KB)

Inhibitory Antibodies User Guide (602 KB)
References
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Anti-Mouse CD3 Antibody (17A2) Related Classifications

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The dilution calculator equation

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse CD3 Antibody (17A2)CD3Cluster of differentiation 3T cellsIFN γIL-17IRBPGVHDInhibitorinhibitorinhibit

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