2. Immunology/Inflammation
  3. CD28
  4. Anti-Mouse CD28 Antibody (D665)

Anti-Mouse CD28 Antibody (D665) is a mouse-derived agonistic IgG1 κ type antibody, targeting to mouse CD28. Anti-Mouse CD28 Antibody (D665) induces the activation of T cells. Anti-Mouse CD28 Antibody (D665) can be used for the researches of neurological disease, inflammation, immunology and cancer, such as Parkinson disease (PD), arthritis and graft-versus-host disease (GVHD).

For research use only. We do not sell to patients.

Anti-Mouse CD28 Antibody (D665)

Anti-Mouse CD28 Antibody (D665) Chemical Structure

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Description

Anti-Mouse CD28 Antibody (D665) is a mouse-derived agonistic IgG1 κ type antibody, targeting to mouse CD28. Anti-Mouse CD28 Antibody (D665) induces the activation of T cells. Anti-Mouse CD28 Antibody (D665) can be used for the researches of neurological disease, inflammation, immunology and cancer, such as Parkinson disease (PD), arthritis and graft-versus-host disease (GVHD)[1][2][3][4].

Isotype

Mouse IgG1 kappa
Species Reactivity

Mouse
IC50 & Target

CD28
In Vivo

Anti-Mouse CD28 Antibody (D665) (200 μg, i.p., at days 7) attenuates motor deficits, ameliorates SN dopaminergic cell death and suppresses inflammation in hαSyn-injected Parkinson disease (PD) mice[1].
Anti-Mouse CD28 Antibody (D665) (100 μg, i.p., at days 7) reduces arthritis severity in glucose-6-phosphate isomerase (G6PI)-induced arthritis mice[2].
Anti-Mouse CD28 Antibody (D665) (250 μg, i.p., 3 days before isolation of splenic CD4+ cells) prevents graft-versus-host disease in CD4+ cells from C57BL/6 mice transferred BALB/c mice[3].
Anti-Mouse CD28 Antibody (D665) (250 μg, i.p., 3 days before isolation of the T cells from total splenocytes) prolongs survival in T cells transferred BCL lymphoma mice[3].
Anti-Mouse CD28 Antibody (D665) (50 μg/mouse, i.p., 3 days before tMCAO) increases regulatory T cells (Treg) and increases thrombo-inflammation and ischemic neurodegeneration in transient middle cerebral artery occlusion (tMCAO) mice models[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: HαSyn-injected PD mice[1]
Dosage: 200 μg
Administration: Intraperitoneally injection, at days 7
Result: Increased Treg in cervical lymph nodes and spleen.
Showed a significant elevation of CD4+CD25+FoxP3+ Treg.
Revealed an expansion of Treg in brain.
Increased IL-10 levels in cervical lymph nodes and spleen.
Prolonged latency to fall from the rotarod.
Prevented the loss of dopaminergic and total Nissl+ neurons in the SN.
Reduced numbers of CD4+, CD8+ T cells and CD11b+ myeloid cells.
Animal Model: Glucose-6-phosphate isomerase (G6PI)-induced arthritis mice[2]
Dosage: 100 μg
Administration: Intraperitoneally injection, at days 7
Result: Reduced arthritis severity scores.
Showed no infiltration of neutrophils, macrophages or lymphocytes into the joints and cartilage destruction.
Increased CD4+CD25+Foxp3+ Treg cells.
Reduced the percentage of Th cells producing TNF-α, IL-17 or RANKL.
Increased Treg cells in both spleens and lymph nodes and levels of CTLA4 expression and proliferation.
Gene ID

12487  [NCBI]

Accession
Application

ELISA, FACS, Functional assay, Research in vivo
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Molecular Weight

150 kDa

SMILES

[Anti-Mouse CD28 Antibody (D665)]
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Anti-Mouse CD28 Antibody (D665) Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse CD28 Antibody (D665)CD28T cellsParkinson diseaseArthritisGraft-versus-host diseaseInhibitorinhibitorinhibit

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