Anti-Mouse CD27 Antibody (RM27-3E5)

Cat. No.: HY-P990280: Data Sheet
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Anti-Mouse CD27 Antibody (RM27-3E5) is an agonistic rat-derived IgG2a κ type antibody, targeting to mouse CD27. Anti-Mouse CD27 Antibody (RM27-3E5) stimulates CD 27. Anti-Mouse CD27 Antibody (RM27-3E5) can be used for the researches of cancer and immunology, such as B16cOVA tumor.

For research use only. We do not sell to patients.

Anti-Mouse CD27 Antibody (RM27-3E5) Chemical Structure

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•Related Small Molecules:

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TNFRSF1A/CD120a TNFRSF3/CD18 TNFRSF4 TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF12A/TWEAK TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

Biological Activity
Purity & Documentation
References
Customer Review

Description

Isotype

Rat IgG2a kappa

Recommend Isotype Controls

Rat IgG2a kappa, Isotype Control

Species Reactivity

Mouse

IC₅₀ & Target

CD27

In Vitro

The antibody framework is stable, specific and adaptable, and has the ability to bind both antigens and endogenous immune receptors. Monoclonal antibodies have several derivatives, including bispecific antibodies, antibody-drug conjugates, and antibody fragments, and have significant effects in fields such as immunology and oncology. When designing inhibitory antibodies, considerations include identification of antigen-specific variable regions, choice of expression system, use of multispecific formats, and antibody derivatives based on fragmentation, oligomerization, or conjugation with other functional moieties^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Anti-Mouse CD27 Antibody (RM27-3E5) (100 μg, i.p., every 3-4 days from day 7) reduces tumor nodules in B16cOVA/B16F1 experimental tumor metastases mice models^[1].
Anti-Mouse CD27 Antibody (RM27-3E5) (100 μg, i.p., at days 5, 8, and 11) enhances cytotoxic T lymphocytes response and antitumor immunity companied with anti-CTLA-4/anti-PD-1 in TC-1 tumor mice models^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	B16cOVA/B16F1 experimental metastases mice models^[1]
Dosage:	100 μg
Administration:	Intraperitoneally injection, every 3-4 days from day 7
Result:	Reduced tumor nodules. Increased the proportion of OVA257-specific CD8⁺ T cells. Increased IFNγ-expressing cells. Enhanced or sustained IFNγ-production by tumor infiltrating CD8⁺ T cells and NK1.1⁺ cells. Reduced the proportion of FoxP3⁺ CD4⁺ T cells.

Animal Model:	TC-1 tumor mice models^[2]
Dosage:	100 μg
Administration:	Intraperitoneally injection, daily, companied with anti-CTLA-4/anti-PD-1
Result:	Improved cytotoxic T lymphocytes priming and prolonged survival. Increased cytotoxic T lymphocytes numbers.

Gene ID

21940 [NCBI]

Accession

P41272

Application

ELISA, FACS, Functional assay, Research in vivo

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Molecular Weight

150 kDa

SMILES

[Anti-Mouse CD27 Antibody (RM27-3E5)]

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Inhibitory Antibodies User Guide (602 KB)

References

[1]. Roberts DJ, et al. Control of established melanoma by CD27 stimulation is associated with enhanced effector function and persistence, and reduced PD-1 expression of tumor infiltrating CD8(+) T cells. J Immunother. 2010 Oct;33(8):769-79. [Content Brief]

[2]. Ahrends T, et al. CD27 Agonism Plus PD-1 Blockade Recapitulates CD4+ T-cell Help in Therapeutic Anticancer Vaccination. Cancer Res. 2016 May 15;76(10):2921-31. [Content Brief]

Anti-Mouse CD27 Antibody (RM27-3E5) Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse CD27 Antibody (RM27-3E5)TNF ReceptorTransmembrane GlycoproteinTumor Necrosis Factor ReceptorTNFRCD27B16cOVAB16F1TC-1T cellsNK cellsCytotoxic T lymphocytesInhibitorinhibitorinhibit

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