Anti-Mouse CD16/CD32 Antibody (2.4G2) 

Anti-Mouse CD16/CD32 Antibody (2.4G2) is an anti-mouse CD16/CD32 IgG2b monoclonal antibody. Anti-Mouse CD16/CD32 Antibody (2.4G2) can block Fcγ receptor-mediated immune responses. Anti-Mouse CD16/CD32 Antibody (2.4G2) can block the combination of CD16 and FcγRs to reduce the production of foam cells. Anti-Mouse CD16/CD32 Antibody (2.4G2) can be used for research on atherosclerosis and autoimmune hematological disorders^[1][2][3][4].

Rat IgG2b kappa

Rat IgG2b kappa, Isotype Control

Anti-Mouse CD16/CD32 Antibody (2.4G2) significantly inhibits the binding of MDALDL to J774 cells^[1].
Anti-Mouse CD16/CD32 Antibody (2.4G2) (24 h) significantly reduces the production of foam cells by blocking FcγRs in RAW264.7 cells^[1].
Anti-Mouse CD16/CD32 Antibody (2.4G2) (0.01 mg/mL, 6 days) inhibits anti-body secreting cells (ASC) formation and also reduces T cell cytokine levels such as IFN-γ, IL-10 and TNF-α in T cells from the spleen of F8^-/- mice^[2].
Anti-Mouse CD16/CD32 Antibody (2.4G2) (0.01 mg/mL, 4 days) interferes with the uptake and presentation of FVIII immune complexes by B cells from the spleen of F8^-/- mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Mouse CD16/CD32 Antibody (2.4G2) (8 μg/g, i.v., single dose, 1 h before injection of MAb 6A6) can inhibit platelet clearance in γ^-/- mice^[3].
Anti-Mouse CD16/CD32 Antibody (2.4G2) (100 μg, i.p., single dose, 24 h before injection of MWReg30) reverses the pathological effects of rat antimouse platelet MWReg30 in NMRI mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA, FACS, Functional assay, Research in vivo

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

150 kDa

Liquid

Colorless to light yellow

[Anti-Mouse CD16/CD32 Antibody (2.4G2)]

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Zhu X, et al. Scavenger receptor function of mouse Fcγ receptor III contributes to progression of atherosclerosis in apolipoprotein E hyperlipidemic mice. J Immunol. 2014 Sep 1;193(5):2483-95.  [Content Brief]

  [2]. Vollack N, et al. CD32 inhibition and high dose of rhFVIII suppress murine FVIII-specific recall response by distinct mechanisms in vitro. Thromb Haemost. 2017 Aug 30;117(9):1679-1687.  [Content Brief]

  [3]. Clynes R, et al. Cytotoxic antibodies trigger inflammation through Fc receptors. Immunity. 1995 Jul;3(1):21-6.  [Content Brief]

  [4]. Nieswandt B, et al. Acute systemic reaction and lung alterations induced by an antiplatelet integrin gpIIb/IIIa antibody in mice. Blood. 1999 Jul 15;94(2):684-93.  [Content Brief]