Anonaine

Name: Anonaine
Brand: MedChemExpress
SKU: HY-N7227
Rating: 4.5 (1 reviews)

Cat. No.: HY-N7227 Purity: ≥95.0%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Anonaine is an antiparasitic agent. Anonaine also is a glutathione S-transferase (GST) inhibitor.

For research use only. We do not sell to patients.

Anonaine Chemical Structure

CAS No. : 1862-41-5

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	Get quote
10 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All Parasite Isoform Specific Products:

View All Isoforms

Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

Biological Activity
Purity & Documentation
References
Customer Review

Description

Anonaine is an antiparasitic agent. Anonaine also is a glutathione S-transferase (GST) inhibitor^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A-431	ED₅₀	4.2 μg/mL Compound: 3	Cytotoxicity against human A431 cells after 3 days by SRB assay Cytotoxicity against human A431 cells after 3 days by SRB assay	[PMID: 8254346]
AGS	IC₅₀	8.6 μM Compound: 5	Cytotoxicity against human AGS cells by MTS colorimetric assay Cytotoxicity against human AGS cells by MTS colorimetric assay	[PMID: 11087586]
DLD-1	IC₅₀	28.9 μM Compound: 5	Cytotoxicity against human DLD1 cells by MTS colorimetric assay Cytotoxicity against human DLD1 cells by MTS colorimetric assay	[PMID: 11087586]
HeLa	IC₅₀	24.8 μg/mL Compound: Anonaine, III	Cytotoxicity against human HeLa cells after 24 hrs by MTT assay Cytotoxicity against human HeLa cells after 24 hrs by MTT assay	[PMID: 18295494]
Hepatocyte	IC₅₀	70.3 μg/mL Compound: Anonaine, III	Cytotoxicity against Sprague-Dawley rat primary hepatocytes after 24 hrs by MTT assay Cytotoxicity against Sprague-Dawley rat primary hepatocytes after 24 hrs by MTT assay	[PMID: 18295494]
HepG2	IC₅₀	20.8 μM Compound: 5	Cytotoxicity against human HepG2 cells by MTS colorimetric assay Cytotoxicity against human HepG2 cells by MTS colorimetric assay	[PMID: 11087586]
HepG2	IC₅₀	33.5 μg/mL Compound: Anonaine, III	Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 18295494]
HT-1080	ED₅₀	2.4 μg/mL Compound: 3	Cytotoxicity against human HT1080 cells after 3 days by SRB assay Cytotoxicity against human HT1080 cells after 3 days by SRB assay	[PMID: 8254346]
KB	ED₅₀	2.6 μg/mL Compound: 3	Cytotoxicity against human KB cells after 3 days by SRB assay Cytotoxicity against human KB cells after 3 days by SRB assay	[PMID: 8254346]
KB-V1	ED₅₀	1.8 μg/mL Compound: 3	Cytotoxicity against vinblastine-resistant human KBV1 cells after 3 days by SRB assay Cytotoxicity against vinblastine-resistant human KBV1 cells after 3 days by SRB assay	[PMID: 8254346]
LNCaP	ED₅₀	2.9 μg/mL Compound: 3	Cytotoxicity against human LNCAP cells after 3 days by SRB assay Cytotoxicity against human LNCAP cells after 3 days by SRB assay	[PMID: 8254346]
P388	ED₅₀	> 5 μg/mL Compound: 3	Cytotoxicity against mouse P388 cells after 3 days by SRB assay Cytotoxicity against mouse P388 cells after 3 days by SRB assay	[PMID: 8254346]
SK-MEL-2	ED₅₀	15.8 μg/mL Compound: 3	Cytotoxicity against human SK-MEL-2 cells after 3 days by SRB assay Cytotoxicity against human SK-MEL-2 cells after 3 days by SRB assay	[PMID: 8254346]
U-373MG ATCC	ED₅₀	1.5 μg/mL Compound: 3	Cytotoxicity against human U373 cells after 3 days by SRB assay Cytotoxicity against human U373 cells after 3 days by SRB assay	[PMID: 8254346]
ZR-75-1	ED₅₀	2.7 μg/mL Compound: 3	Cytotoxicity against human ZR-75-1 cells after 3 days by SRB assay Cytotoxicity against human ZR-75-1 cells after 3 days by SRB assay	[PMID: 8254346]

Molecular Weight

265.31

Formula

C₁₇H₁₅NO₂

CAS No.

1862-41-5

Appearance

Solid

Color

Light brown to brown

SMILES

[H][C@@]1(NCC2)CC3=CC=CC=C3C4=C1C2=CC5=C4OCO5

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 50 mg/mL (188.46 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7692 mL	18.8459 mL	37.6918 mL
5 mM	0.7538 mL	3.7692 mL	7.5384 mL
10 mM	0.3769 mL	1.8846 mL	3.7692 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (4.71 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 1.25 mg/mL (4.71 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 1.25 mg/mL (4.71 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥95.0%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (258 KB) HNMR (251 KB) RP-HPLC (172 KB)

Handling Instructions (2659 KB)

References

[1]. Bezerra WADS, et al. Anonaine from Annona crassiflora inhibits glutathione S-transferase and improves cypermethrin activity on Rhipicephalus (Boophilus) microplus (Canestrini, 1887). Exp Parasitol. 2022 Dec;243:108398. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.7692 mL	18.8459 mL	37.6918 mL	94.2294 mL
	5 mM	0.7538 mL	3.7692 mL	7.5384 mL	18.8459 mL
	10 mM	0.3769 mL	1.8846 mL	3.7692 mL	9.4229 mL
	15 mM	0.2513 mL	1.2564 mL	2.5128 mL	6.2820 mL
	20 mM	0.1885 mL	0.9423 mL	1.8846 mL	4.7115 mL
	25 mM	0.1508 mL	0.7538 mL	1.5077 mL	3.7692 mL
	30 mM	0.1256 mL	0.6282 mL	1.2564 mL	3.1410 mL
	40 mM	0.0942 mL	0.4711 mL	0.9423 mL	2.3557 mL
	50 mM	0.0754 mL	0.3769 mL	0.7538 mL	1.8846 mL
	60 mM	0.0628 mL	0.3141 mL	0.6282 mL	1.5705 mL
	80 mM	0.0471 mL	0.2356 mL	0.4711 mL	1.1779 mL
	100 mM	0.0377 mL	0.1885 mL	0.3769 mL	0.9423 mL

Anonaine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anonaine1862-41-5Glutathione S-transferaseParasiteGlutathione transferasesGSTsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Anonaine

Customer Review

Anonaine Related Antibodies

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All Parasite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Anonaine Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report