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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D2643
    Cy5 HA (MW 50000)
    Cy5 HA (MW 50000) is a hyaluronic acid labeled with CY5 (HY-D0821). Cy5 HA (MW 50000) is widely used for cell imaging and in vivo imaging of shallow tissue (Ex/Em = 633/670 nm).
    Cy5 HA (MW 50000)
  • HY-D2687
    Cy7 HA biotin 5000
    Cy7 HA biotin 5000 is a biotinylated hyaluronic acid labeled with CY7 (HY-D0825). Cy7 HA biotin 5000 is widely used for cell imaging and in vivo imaging of shallow tissues (Ex/Em = 740/770 nm).
    Cy7 HA biotin 5000
  • HY-D0415
    C.I. Vat blue 22
    C.I. Vat blue 22 is the most important colorant used to add color or change the color of something and is chemically essentially unaffected by the carrier or medium into which it is incorporated. It can be widely used in textile, medicine, food, cosmetics, plastics, paint, ink, photography and paper industries.
    C.I. Vat blue 22
  • HY-D2509
    Cy3-PEG-SH (MW 1000)
    Cy3-PEG-SH (MW 1000) is a fluorescent labeling reagent that combines Cy3 (HY-D0822) fluorescent dye, polyethylene glycol (PEG) and sulfhydryl (SH) derivatives, and is suitable for molecular coupling. SH is highly reactive and can react with a variety of functional groups (such as maleimide) to form stable thioether bonds.
    Cy3-PEG-SH (MW 1000)
  • HY-D0546
    Solvent yellow 124
    Solvent yellow 124 is a yellow dye. Its series of products, such as Solvent orange 60 (HY-D1177), has been used in dyeing applications of plastic materials.
    Solvent yellow 124
  • HY-D0418
    C.I. Acid yellow 42
    C.I. Acid yellow 42 is an acidic yellow dye that can be adsorbed and removed by bentone.
    C.I. Acid yellow 42
  • HY-D1414
    Cyanine 3 Bisfunctional MTSEA Dye potassium
    Cyanine 3 Bisfunctional MTSEA Dye potassium belongs to the cyanine dye series and is a common fluorescent marker for biomolecules that can interact with biomolecules. Cyanine dyes may also bind to double-helical DNA through intercalation and exhibit enhanced fluorescence upon binding.
    Cyanine 3 Bisfunctional MTSEA Dye potassium
  • HY-D0691
    Reactive yellow 25
    Reactive yellow 25 is common textile dyes that can be adsorbed onto single-walled carbon nanotubes (SWCNTs) through electrostatic interactions, allowing the separation of residual dyes.
    Reactive yellow 25
  • HY-D0347
    Disperse Red 17
    Disperse Red 17 is a red dye derived from anthraquinone. It is used in plastics, textiles and cosmetics.
    Disperse Red 17
  • HY-172166C
    TRITC-lysine-dextran (MW 500kDa)
    TRITC-lysine-dextran, 500kDa is a dextran with lysine coupling with a fluorescent group TRITC (HY-D0791). Lysine-dextran exhibits higher Glu-plasminogen binding ability than dextran alone.
    TRITC-lysine-dextran (MW 500kDa)
  • HY-D2847A
    FITC-PEG-DBCO (MW 3400)
    FITC-PEG-DBCO (MW 3400) is a fluorescent dye composed of FITC (HY-66019), PEG and dibenzocyclooctyne (DBCO). DBCO is a cycloalkyne that can react with molecules containing azide groups through "click chemistry" to achieve coupling (Ex/Em = 488/525 nm).
    FITC-PEG-DBCO (MW 3400)
  • HY-D2611
    ICG PEG DSPE (MW 3400)
    ICG PEG DSPE (MW 3400) is a PEG phospholipid with Indocyanine green (ICG) (HY-D0711) dye used in protein/nucelic acid labeling and fluorescence microscopy. ICG PEG DSPE (MW 340) can self-assemble in aqueous solution to form micelles/lipid bilayer and used to prepare liposomes or nanoparticles for nutrients delivery such as mRNA or DNA vaccine (Ex/Em = 785/813 nm).
    ICG PEG DSPE (MW 3400)
  • HY-D2660
    Cy5.5 HA (MW 50000)
    Cy5.5 HA (MW 50000) is a hyaluronic acid labeled with CY5.5 (HY-D0924). Cy5.5 HA (MW 50000) is widely used for cell imaging and in vivo imaging of shallow tissue (Ex/Em = 680/710 nm).
    Cy5.5 HA (MW 50000)
  • HY-D0521
    Disperse orange 29
    Disperse orange 29 is an orange-orange dye commonly used for fiber dyeing.
    Disperse orange 29
  • HY-D2613
    ICG PEG DSPE (MW 10000)
    ICG PEG DSPE (MW 10000) is a PEG phospholipid with Indocyanine green (ICG) (HY-D0711) dye used in protein/nucelic acid labeling and fluorescence microscopy. ICG PEG DSPE (MW 10000) can self-assemble in aqueous solution to form micelles/lipid bilayer and used to prepare liposomes or nanoparticles for nutrients delivery such as mRNA or DNA vaccine (Ex/Em = 785/813 nm).
    ICG PEG DSPE (MW 10000)
  • HY-D0613
    Acid Red 337
    Acid Red 337 is an acid dye that can be decolorized when aluminum sulfate is used as a coagulant.
    Acid Red 337
  • HY-D1524
    N-(Aminoethyl)-8-naphthylamine-1-sulfonic acid
    N-(Aminoethyl)-8-naphthylamine-1-sulfonic acid is a fluorescent reagent.
    N-(Aminoethyl)-8-naphthylamine-1-sulfonic acid
  • HY-D2856A
    FITC-PEG-Br (MW 2000)
    FITC-PEG-Br (MW 2000) is a fluorescent dye composed of FITC (HY-66019), PEG and chlorine (-Cl). The chlorine group is an electrophilic active group that can be used for nucleophilic substitution reactions and facilitate subsequent chemical modification (Ex/Em = 488/525 nm).
    FITC-PEG-Br (MW 2000)
  • HY-D2773
    5-Carboxyrhodamine 110 NHS ester
    5-Carboxyrhodamine 110 NHS Ester is the nonsulfonated analog of the BP Fluor 488 dye. The amine-reactive 5(6)-Carboxyrhodamine 110 NHS Ester can be used to create bright and photostable green-fluorescent bioconjugates with excitation/emission maxima ~502/527 nm. For many applications, the dye is preferred over 5-(6)-carboxyfluorescein NHS ester or FITC because of its exceptional photostability and fluorescence insensitivity to pH (4-9). Although the mixed isomers of Carboxyrhodamine 110 NHS Ester preferred, routinely used fluorescent dye for labeling proteins, peptides and nucleotides, purification of peptide and nucleotides labeled with 5(6) isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer usually give better resolution in HPLC purification that is often required in the conjugation processes.
    5-Carboxyrhodamine 110 NHS ester
  • HY-D0351
    Fluorescent Brightener 134
    Fluorescent Brightener 134 is an optical brightener dye.
    Fluorescent Brightener 134
Cat. No. Product Name / Synonyms Application Reactivity