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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D2444
    AF555 NHS
    AF555 NHS is a red fluorescent dye with excellent fluorescence properties and light stability. The excitation wavelength is 556 nm and the emission wavelength is 571 nm, which can be used for protein labeling, antibody labeling, and cell imaging.
    AF555 NHS
  • HY-D2550
    Cy5-PEG-FA (MW 2000)
    Cy5-PEG-FA (MW 2000) is a fluorescently labeled biomolecule consisting of Cy5 (HY-D0821) fluorescent dye, polyethylene glycol (PEG), and Folic acid (FA) (HY-16637). Cy5 is commonly used in applications such as immunolabeling and nucleic acid labeling. The maximum emission wavelength of Cy5 is approximately 670 nm.
    Cy5-PEG-FA (MW 2000)
  • HY-D2539
    Cy3-PEG-N3 (MW 5000)
    Cy3-PEG-N3 (MW 5000) is a Cy3 (HY-D0822)-azide fluorescent dye used to label for protein and nucleic acid. Cy3-PEG-N3 (MW 5000) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    Cy3-PEG-N3 (MW 5000)
  • HY-D2532
    Cy5-PEG-SH (MW 10000)
    Cy5-PEG-SH (MW 10000) is a fluorescent labeling reagent that combines Cy5 (HY-D0821) fluorescent dye, polyethylene glycol (PEG) and sulfhydryl (SH) derivatives, and is suitable for molecular coupling. SH is highly reactive and can react with a variety of functional groups (such as maleimide) to form stable thioether bonds.
    Cy5-PEG-SH (MW 10000)
  • HY-D2600
    ICG PEG NH2 (MW 2000)
    ICG PEG NH2 (MW 2000) is a polyethylene glycol derivative containing Indocyanine green (ICG) (HY-D0711) fluorescent dye and polyethylene glycol (PEG) and an amino group. The Indocyanine green fluorophore is commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. ICG PEG NH2 (MW 2000) can be used for fluorescence imaging, fluorescence tracing and fluorescence labeling (Ex/Em = 785/813 nm).
    ICG PEG NH2 (MW 2000)
  • HY-D0715R
    Fluorescamine (Standard)
    Fluorescamine (Standard) is the analytical standard of Fluorescamine (HY-D0715). This product is intended for research and analytical applications. Fluorescamine is a spirocyclic compound that is non-fluorescent. Fluorescamine reacts rapidly with primary amine groups in proteins under alkaline conditions to generate products with strong fluorescence (Ex/Em : 390/475 nm). Fluorescamine can be used to detect amine-containing compounds, including amino acids, peptides, and proteins.
    Fluorescamine (Standard)
  • HY-D2560
    Cy5.5-PEG-N3 (MW 2000)
    Cy5.5-PEG-N3 (MW 2000) is a Cy5.5 (HY-D0924)-azide fluorescent dye used to label for protein and nucleic acid. Cy5.5-PEG-N3 (MW 2000) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    Cy5.5-PEG-N3 (MW 2000)
  • HY-D2636
    Cy3 HA biotin 5000
    Cy3 HA biotin 5000 is a biotinylated hyaluronic acid labeled with CY3 (HY-D0822). Cy3 HA biotin 5000 is widely used for cell imaging and in vivo imaging of shallow tissues (Ex/Em = 550/570 nm).
    Cy3 HA biotin 5000
  • HY-D2846
    m-PEG-PEI800-Cy5
    m-PEG-PEI800-Cy5 is a fluorescent dye composed of CY5 (HY-D0821), methoxypolyethylene glycol (mPEG), and polyethyleneimine (PEI). m-PEG-PEI8800-Cy5 is used for gene delivery, nanodrug delivery, cell imaging and biosensing and bioanalysis (Ex/Em = 633/670 nm).
    m-PEG-PEI800-Cy5
  • HY-D0364
    Disperse Orange 44
    Disperse Orange 44 is an orange-orange dye commonly used for fiber dyeing.
    Disperse Orange 44
  • HY-D0644
    Disperse blue 102
    Disperse blue 102 is a yellow dye commonly used for fiber dyeing.
    Disperse blue 102
  • HY-D0658
    Direct blue 85
    Direct blue 85 is a good dyeing agent for cotton fabrics.
    Direct blue 85
  • HY-D2590
    Cy7.5 PEG-N3
    Cy7.5 PEG-N3 is a CY7.5 (HY-D0926)-azide fluorescent dye used to label for protein and nucleic acid. Cy7.5 PEG-N3 is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups (Ex/Em = 770/820 nm).
    Cy7.5 PEG-N3
  • HY-D2544
    Cy5-PEG-DSPE (MW 5000)
    Cy5-PEG-DSPE (MW 5000) is a PEG phospholipid with Cy5 (HY-D0821) dye used in protein/nucelic acid labeling and fluorescence microscopy. Cy5-PEG-DSPE (MW 5000) can self-assemble in aqueous solution to form micelles/lipid bilayer and used to prepare liposomes or nanoparticles for nutrients delivery such as mRNA or DNA vaccine.
    Cy5-PEG-DSPE (MW 5000)
  • HY-160240
    HPI
    HPI is a HS- fluorescent probe (Ex/Em=280/410 nm). HPI can be used to detect the HS- form of hydrogen sulfide (H2S) gas in environmental water samples.
    HPI
  • HY-D0423
    C.I. Acid green 27
    C.I. Acid green 27 is an acidic green dye that can be adsorbed and removed by bentone.
    C.I. Acid green 27
  • HY-160278
    DSPE-PEG-Fluor 647,MW 2000
    DSPE-PEG-Fluor 647, MW 2000 is a fluorescent lipid (Ex/Em=648/671 nm) composed of PEG-lipid-dye conjugation. DSPE is a phospholipid. Fluor 647 is a fluorescent dye that can be used for microscopy in vivo.
    DSPE-PEG-Fluor 647,MW 2000
  • HY-D2702
    Cy7.5 HA biotin (MW 3000)
    Cy7.5 HA biotin (MW 3000) is a biotinylated hyaluronic acid labeled with CY7.5 (HY-D0926). Cy7.5 HA biotin (MW 3000) is widely used for cell imaging and in vivo imaging of shallow tissues (Ex/Em = 770/820 nm).
    Cy7.5 HA biotin (MW 3000)
  • HY-W781921
    BP Fluor 488 Alkyne
    BP Fluor 488 Alkyne is a popular labeling dye used in Click Chemistry reactions. It will react with the azide group in antibody, proteins, peptides, amino-modified oligos, and other target molecules. The dye has an excitation peak at 499 nm and an emission peak at 520 nm. The conjugates are widely used in microscopy, flow cytometry, and other applications. BP Fluor 488 is a pure 5-sulfonated rhodamine molecule and it eliminates the lot-to-lot variation caused by two isomers ratio differences.
    BP Fluor 488 Alkyne
  • HY-D1349
    Bodipy TR alkyne
    Bodipy TR alkyneis one of a boron dipyrromethene fluorophore for the ROX (Texas Red) channel. This is a versatile fluorophore that can be used in microscopy, fluorescence polarization measurements, and other applications. This derivative is a terminal alkyne of copper-catalyzed click chemistry.
    Bodipy TR alkyne
Cat. No. Product Name / Synonyms Application Reactivity