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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-15558A
    Hoechst 33258 trihydrochloride
    		99.51%
    Hoechst 33258 trihydrochloride is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
    Hoechst 33258 trihydrochloride
  • HY-D0944
    Giemsa stain
    Giemsa stain can stain chromatin and nuclear membrane. Giemsa stain histopathologic detection of malaria and other microorganisms, such as Histoplasma, LeishmaniaToxoplasma, and Pneumocystis.
    Giemsa stain
  • HY-D2295
    Mito-FerroGreen
    Mito-FerroGreen is a mitochondria-specific ferrous ion detection probe with an excitation wavelength of 488nm.
    Mito-FerroGreen
  • HY-D0945
    Xylene Cyanol FF
    Xylene Cyanol FF is an acid triphenylmethane dye. Xylene Cyanol FF can be used for histochemical staining of hemoglobin peroxidase or as a tracking dye for DNA sequencing in electrophoresis. Xylene Cyanol FF will be catalyzed by Fe and Al to accelerate oxidation under the addition of double oxidant hydrogen peroxide and potassium periodate. Xylene Cyanol FF thus enables the spectrophotometric determination of Fe and Al in the solution to be tested.
    Xylene Cyanol FF
  • HY-148263
    Biotin-PEG-Thiol (MW 2000)
    		≥98.0%
    Biotin-PEG-Thiol (MW 2000) is an active compound. Biotin-PEG-Thiol (MW 2000) is pegylated by binding to streptavidin or antibiotin with high affinity and specificity. Biotin-PEG-Thiol (MW 2000) can modify biomolecules, proteins, peptides and other small molecule materials. Biotin-PEG-Thiol (MW 2000) is widely used in the research of agent release and nano new materials.
    Biotin-PEG-Thiol (MW 2000)
  • HY-D1117
    NADA-green
    NADA-green is a fluorescent D-amino acid probe. NADA-green is efficiently incorporated into the peptidoglycan of diverse bacterial species peptidoglycan biosynthesis. NADA-green allows probing of bacterial growth with minimal perturbation.
    NADA-green
  • HY-D0893
    NSP-SA-NHS
    		99.65%
    NSP-SA-NHS is an acridinium ester that can be used for chemiluminescent immunoassay. A rapid and sensitive chemiluminescent immunoassay of Bisphenol A (BPA) with NSP-SA-NHS-labeled has been developed.
    NSP-SA-NHS
  • HY-W400925
    Sulfo-Cy5-N3
    		99.12%
    Sulfo-Cy5-N3 is a click chemistry reagent containing an azide group. Cy5 is a red fluorescent label dye for imaging (Ex/Em=642/670 nm). It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.
    Sulfo-Cy5-N3
  • HY-D0193
    Ponceau 4R
    		99.84%
    Ponceau 4R is a synthetic colorant that can be used for food coloring. Ponceau 4R is a strawberry red azo dye that can be used in a variety of foods, usually synthesized from aromatic hydrocarbons, and is stable to light, heat, and acids.
    Ponceau 4R
  • HY-D0022
    5-Aminofluorescein
    		99.66%
    5-Aminofluorescein (5-AF) is a fluorescence marker, covalently bound to human serum albumin. The excitation wavelength is 495 nm and the emission wavelength is 535 nm.
    5-Aminofluorescein
  • HY-D1834
    Vari Fluor 640 TSA（200×）
    Vari Fluor 640 TSA（200×） (VF 640 Tyramide) is a fluorescent probe belonging to the Vari Fluor TSA series. Vari Fluor TSA series fluorescent probes can be used for high-density in situ labeling of target antigens using horseradish peroxidase (HRP) assays, which are widely used in ELISA, IF, IHC and other techniques with high detection sensitivity.
    Vari Fluor 640 TSA（200×）
  • HY-D1835
    Vari Fluor 594 TSA（200×）
    Vari Fluor 594 TSA（200×） (VF 594 Tyramide) is a fluorescent probe belonging to the Vari Fluor TSA series. Vari Fluor TSA series fluorescent probes can be used for high-density in situ labeling of target antigens using horseradish peroxidase (HRP) assays, which are widely used in ELISA, IF, IHC and other techniques with high detection sensitivity.
    Vari Fluor 594 TSA（200×）
  • HY-D1903
    Fura Red AM
    Fura Red AM is a fluorescent probe that can be used to visualize Ca2+ distribution in the cytoplasm of undifferentiated cells. Fura Red AM operates at an excitation wavelength of 488 nm.
    Fura Red AM
  • HY-137845
    4-Methylumbelliferyl β-D-galactopyranoside
    		99.06%
    4-Methylumbelliferyl β-D-galactopyranoside is a fluorescent substrate for β-galactosidase which, when cleaved, produces a water-soluble blue fluorescent coumarin fluorophore that can be detected using a fluoroenzymeter or fluorometer.
    4-Methylumbelliferyl β-D-galactopyranoside
  • HY-D0817
    Rhodamine 110
    		99.81%
    Rhodamine 110 is a sensitive and selective substrate for assaying proteinases in solution or inside living cells. The excitation wavelength is 498 nm and the emission wavelength is 521 nm.
    Rhodamine 110
  • HY-D0047
    5-CFDA
    		98.22%
    5-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase. CF containing portion contains an additional negative charge so that it is better retained in cells, compared to fluorescein dyes.
    5-CFDA
  • HY-D1711
    MHI-148
    		98.39%
    MHI-148 is a near-infrared heptamethine cyanine dye with tumor-targeting properties for cancer detection, diagnosis and research. MHI-148 is immediately taken up and accumulated by lysosomes and mitochondria of tumor cells, but not in lysosomes and mitochondria of normal cells.
    MHI-148
  • HY-D0935A
    4-Methylumbelliferyl-β-D-glucuronide hydrate
    		99.92%
    4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λex=362 nm, λem=445 nm). 4-Methylumbelliferyl-β-D-glucuronide hydrate has potential applications in detecting the activity of β-glucuronidase and the number of Escherichia coli.
    4-Methylumbelliferyl-β-D-glucuronide hydrate
  • HY-132969
    SBP-3264
    		98.50%
    SBP-3264 is a valuable chemical probe for understanding the roles of STK3 and STK4 in acute myeloid leukemia (AML).
    SBP-3264
  • HY-136865
    MeOSuc-AAPV-AMC
    		98.07%
    MeOSuc-AAPV-AMC is a fluorogenic substrate for human leukocyte and porcine pancreatic elastase (Km: 362 μM, Ex=380 nm, Em=460 nm).
    MeOSuc-AAPV-AMC
Cat. No. Product Name / Synonyms Application Reactivity