2. Membrane Transporter/Ion Channel Others
  3. Na+/K+ ATPase Fluorescent Dye
  4. Oxonol VI

Oxonol VI is an optical indicator of membrane potential in lipid vesicles (excitation/emission wavelengths: 614/646 nm). Oxonol VI can be used to detect changes in membrane potential associated with (Na+ + K+)-ATPase activity in reconstituted vesicles.

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Oxonol VI

Oxonol VI Chemical Structure

CAS No. : 64724-75-0

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Oxonol VI Related Antibodies

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Description

Oxonol VI is an optical indicator of membrane potential in lipid vesicles (excitation/emission wavelengths: 614/646 nm). Oxonol VI can be used to detect changes in membrane potential associated with (Na+ + K+)-ATPase activity in reconstituted vesicles[1][2].

In Vitro

Guidelines (The following is our recommended protocol. This protocol is for guidance only and should be modified according to your specific needs).
1 Preparation of dye stock solution [1]
1) Prepare a 3.16 mM Oxonol VI stock solution in ethanol.
2 Preparation of dye working solution
1) Dilute the 3.16 mM Oxonol VI stock solution with a mixture of ethanol and water at a volume ratio of 1:5 (ethanol:water).
2) Adjust the dilution ratio to ensure that the final concentration of Oxonol VI reaches the range of 10-500 nM.
3. Specific steps
1) Add 1 mL of the appropriate buffer to the fluorescence cuvette and equilibrate to the set temperature (20 °C by default unless otherwise specified).
2) Measure the background fluorescence intensity of the buffer in the cuvette.
3) Add 5 μL of the prepared Oxonol VI working solution to the cuvette containing the equilibrated buffer.
4) After the fluorescence signal stabilizes, add a predetermined amount of vesicle suspension to the cuvette.
5) Continuously monitor the fluorescence signal using a spectrophotometer. Calculate the relative fluorescence change caused by the addition of the vesicle suspension, using the baseline fluorescence intensity recorded before the addition of the vesicles as a reference.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Oxonol VI Related Antibodies
Molecular Weight

316.35

Formula

C17H20N2O4
CAS No.
Appearance

Solid

Color

Brown to black

Emission (Em)

630
Excitation (Ex)

523
SMILES

O=C1ON=C(CCC)/C1=C\C=C\C=C\C2=C(ON=C2CCC)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (316.11 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1611 mL 15.8053 mL 31.6106 mL
5 mM 0.6322 mL 3.1611 mL 6.3221 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.91%

SDS (252 KB)

COA (251 KB) HNMR (243 KB) RP-HPLC (252 KB) LCMS (101 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1611 mL 15.8053 mL 31.6106 mL 79.0264 mL
5 mM 0.6322 mL 3.1611 mL 6.3221 mL 15.8053 mL
10 mM 0.3161 mL 1.5805 mL 3.1611 mL 7.9026 mL
15 mM 0.2107 mL 1.0537 mL 2.1074 mL 5.2684 mL
20 mM 0.1581 mL 0.7903 mL 1.5805 mL 3.9513 mL
25 mM 0.1264 mL 0.6322 mL 1.2644 mL 3.1611 mL
30 mM 0.1054 mL 0.5268 mL 1.0537 mL 2.6342 mL
40 mM 0.0790 mL 0.3951 mL 0.7903 mL 1.9757 mL
50 mM 0.0632 mL 0.3161 mL 0.6322 mL 1.5805 mL
60 mM 0.0527 mL 0.2634 mL 0.5268 mL 1.3171 mL
80 mM 0.0395 mL 0.1976 mL 0.3951 mL 0.9878 mL
100 mM 0.0316 mL 0.1581 mL 0.3161 mL 0.7903 mL
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Oxonol VI Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Oxonol VI64724-75-0Na+/K+ ATPaseFluorescent DyeSodium potassium pumpoptical indicatormembrane potentialslipid vesiclesNa+K+ATPaseInhibitorinhibitorinhibit

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