CellTracker CM-DiI  (Synonyms: CM-DiI)

CellTracker CM-DiI (CM-DiI) is a lipophilic fluorochrome that can be used for tracking the migration of lymphocytes. CellTracker CM-DiI is feasible to detect labeled cells in histological sections (excitation 553 nm; emission 570 nm)^[1][2][3].

Guidelines (The following is our recommended protocol, which is provided as a guide only and should be modified to suit your specific needs).
Using CM-DiI to label lymphocytes to track their migration^[1]
1 Preparation of stock solution:
1) Dissolve 500 μg of CM-DiI in 250 μL of DMSO.
2 Preparation of working solution:
1) Transfer 7.5 mL of Hank's balanced salt solution preheated to 37°C to the DMSO solution containing CM-DiI and mix for a few seconds until the dye is evenly distributed.
3 Specific staining steps
1) Collect overnight lymph fluid and determine the cell concentration using a Coulter model ZM cell counter. Take a volume containing 1×10⁹ lymphocytes, centrifuge at 4°C, 300×g for 10 min, discard the supernatant, and wash the pellet with HBSS.
2) Resuspend the washed cells in 2.5 mL HBSS and warm to 37°C.
3) Immediately transfer the dye working solution prepared above to the cell suspension and quickly mix by pipetting to a final cell concentration of 1×10⁸ lymphocytes/mL.
4) Incubate the cells at 37°C for 2 min and then on ice for another 2 min.
5) Transfer the cells to a 50 mL centrifuge tube containing 40 mL 4°C HBSS, centrifuge and wash as above. Resuspend the pellet in 20 mL Lactated Ringer's solution, place the cell suspension on ice, and inject within 15-30 min.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1051.49

C₆₈H₁₀₅Cl₂N₃O

180854-97-1

Solid

Orange to red

570

553

CCCCCCCCCCCCCCCCCC[N+]1=C(/C=C/C=C2N(CCCCCCCCCCCCCCCCCC)C3=C(C=CC=C3)C/2(C)C)C(C)(C)C4=C1C=CC(CNC(C5=CC=C(CCl)C=C5)=O)=C4.[Cl-]

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Andrade W, et al. The use of the lipophilic fluorochrome CM-DiI for tracking the migration of lymphocytes. J Immunol Methods. 1996 Aug 14;194(2):181-9.  [Content Brief]

  [2]. Dupuis M, et al. Distribution of adjuvant MF59 and antigen gD2 after intramuscular injection in mice. Vaccine. 1999 Oct 14;18(5-6):434-9.  [Content Brief]

  [3]. Rutten M, et al. Comparison of quantum dots and CM-DiI for labeling porcine autologous bone marrow mononuclear progenitor cells. The Open Stem Cell Journal, 2010, 2(1).