2. Cell Cycle/DNA Damage Epigenetics Autophagy Apoptosis Metabolic Enzyme/Protease
  3. HDAC Autophagy Apoptosis Endogenous Metabolite
  4. Sodium butyrate

Sodium butyrate  (Synonyms: Butanoic acid sodium; Butyric acid sodium)

Cat. No.: HY-B0350A Purity: ≥98.0%
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Sodium Butyrate (sodium butanoate) is an inhibitor of HDAC, possessing anti-tumor activity.

For research use only. We do not sell to patients.

Sodium butyrate

Sodium butyrate Chemical Structure

CAS No. : 156-54-7

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in Water In-stock
Solid
500 mg In-stock
1 g In-stock
5 g In-stock
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Customer Review

Based on 16 publication(s) in Google Scholar

Other Forms of Sodium butyrate:

Sodium butyrate Related Antibodies

Top Publications Citing Use of Products

    Sodium butyrate purchased from MedChemExpress. Usage Cited in: Neurobiol Dis. 2018 Mar:111:12-25.  [Abstract]

    Cdc42-Rac1 activation mediates the effect of SB on microglial process elongation. Representative images showing the activation effect of Cdc42 and Rac1 by Sodium butyrate (SB) (5 mM) at different time points (5, 10 min) in primary cultured microglia.

    View All HDAC Isoform Specific Products:

    View All Isoforms
    HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Sodium Butyrate (sodium butanoate) is an inhibitor of HDAC, possessing anti-tumor activity[1][2][3][4][5].

    IC50 & Target[2]

    HDAC

     

    Human Endogenous Metabolite

     

    Microbial Metabolite

     

    Cellular Effect
    Cell Line Type Value Description References
    Erythrocyte CC50
    222 μM
    Compound: NaB
    Cytotoxicity against human erythrocytes assessed as cell viability after 3 days alamar blue assay
    Cytotoxicity against human erythrocytes assessed as cell viability after 3 days alamar blue assay
    		[PMID: 33109398]
    HeLa IC50
    0.8 mM
    Compound: Sodium butyrate
    Inhibition of HDAC in human Hela cells nuclear extracts by fluorimetric assay
    Inhibition of HDAC in human Hela cells nuclear extracts by fluorimetric assay
    		[PMID: 19520580]
    HUVEC IC50
    1 mM
    Compound: sodium butyrate
    Inhibition of LPS-induced tissue factor activity in HUVEC preincubated for 4 hrs assessed after 4 hrs of LPS challenge by one stage clotting assay
    Inhibition of LPS-induced tissue factor activity in HUVEC preincubated for 4 hrs assessed after 4 hrs of LPS challenge by one stage clotting assay
    		[PMID: 17675290]
    HUVEC IC50
    2 mM
    Compound: sodium butyrate
    Inhibition of TNF-alpha-induced tissue factor activity in HUVEC preincubated for 4 hrs assessed after 4 hrs of TNFalpha challenge by one stage clotting assay
    Inhibition of TNF-alpha-induced tissue factor activity in HUVEC preincubated for 4 hrs assessed after 4 hrs of TNFalpha challenge by one stage clotting assay
    		[PMID: 17675290]
    MDA-MB-231 GI50
    > 1000 μM
    Compound: BA, sodium butyrate
    Growth inhibition of human MDA-MB-231 cells after 5 days by MTT assay
    Growth inhibition of human MDA-MB-231 cells after 5 days by MTT assay
    		[PMID: 18543902]
    PC-3 GI50
    72.44 μM
    Compound: BA, sodium butyrate
    Growth inhibition of human PC3 cells after 5 days by MTT assay
    Growth inhibition of human PC3 cells after 5 days by MTT assay
    		[PMID: 18543902]
    In Vitro

    Sodium Butyrate induces morphological changes, inhibits cell proliferation and impairs cell viability in NPC cells. Sodium Butyrate (1, 5, 10 mM) is cytotoxic to NPC cells, inducing a dose- and time-dependent decrease in cell viability, in both 5-8F and 6-10B cells. Sodium Butyrate induces nasopharyngeal carcinoma cell apoptosis by activating the mitochondrial apoptotic axis. Moreover, SOCE inhibition and disruption of the CRAC channel can attenuate the apoptosis induced by Sodium Butyrate[1].
    Sodium butyrate significantly decreases cell viability, accompanied by reduced levels of p-mTOR and PCNA protein. Sodium butyrate, in a dose-dependent manner, induces cell cycle arrest in G0/G1 phase and reduces the numbers of cells in S phase. In addition, relative expression of p21, p27, and pro-apoptosis bak genes, and protein levels of p21Waf1/Cip1, p27Kip1, cyclinD3, CDK4, and Cleave-caspase3 are increased by higher concentrations of sodium butyrate (1, 5, 10 mM), and the levels of cyclinD1 and CDK6 are reduced by 5 and 10 mM butyrate[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Sodium butyrate Related Antibodies
    In Vivo

    Sodium Butyrate (300 mg/kg, s.c.) administration immediately after HI provided almost complete neuroprotection in comparison with non-treated animals. Sodium butyrate administration results in an increased number of microglial cells to 150% of vehicle-treated animals in the ipsilateral side. Sodium butyrate promotes the polarization of microglia from M1- to M2-like phenotype after neonatal hypoxia-ischemia[2]. Sodium butyrate (300 mg/kg, s.c.) in combination with AK-7 (20 mg/kg, i.p.) significantly alleviates this reduction of the time spent exploring new objects, ameliorates the reduction of the number of Ki67-positive cells and DCX-immunoreactive neuroblasts in the dentate gyrus of the mice. In addition, sodium butyrate reverses SIRT2-related age phenotypes[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    110.09

    Formula

    C4H7NaO2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CCCC(O[Na])=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (908.36 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 9.0836 mL 45.4182 mL 90.8364 mL
    5 mM 1.8167 mL 9.0836 mL 18.1673 mL
    View the Complete Stock Solution Preparation Table

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: ≥ 100 mg/mL (908.36 mM); Clear solution

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: ≥98.0%

    SDS (394 KB)

    COA (244 KB) HNMR (229 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [1]

    Cells are seeded at a density of 2,000 cells/well in 96-well plates with 200 µL culture medium containing Sodium Butyrate at different concentrations. Then, the cells are consecutively cultured for 72 h. Every 24 h, 20 µL 5 mg/mL MTT solution is added into the corresponding well, and the cells are cultured for another 4 h. Then, the solution is replaced with 150 µL dimethylsulfoxide, followed by gentle agitation of the plates for 15 min at room temperature. Finally, the absorbance at 492 nm is measured to represent the cell viability.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [2]

    Seven-day-old rat pups are subjected to unilateral carotid artery ligation followed by 60 min of hypoxia (7.6% O2). Sodium Butyrate (300 mg/kg) is administered in a 5-day regime with the first injection given immediately after hypoxic exposure. The damage of the ipsilateral hemisphere is evaluated by hematoxylin-eosin staining 6 days after the insult. Samples are collected at 24 and 48 h and 6 days.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 9.0836 mL 45.4182 mL 90.8364 mL 227.0911 mL
    5 mM 1.8167 mL 9.0836 mL 18.1673 mL 45.4182 mL
    10 mM 0.9084 mL 4.5418 mL 9.0836 mL 22.7091 mL
    15 mM 0.6056 mL 3.0279 mL 6.0558 mL 15.1394 mL
    20 mM 0.4542 mL 2.2709 mL 4.5418 mL 11.3546 mL
    25 mM 0.3633 mL 1.8167 mL 3.6335 mL 9.0836 mL
    30 mM 0.3028 mL 1.5139 mL 3.0279 mL 7.5697 mL
    40 mM 0.2271 mL 1.1355 mL 2.2709 mL 5.6773 mL
    50 mM 0.1817 mL 0.9084 mL 1.8167 mL 4.5418 mL
    60 mM 0.1514 mL 0.7570 mL 1.5139 mL 3.7849 mL
    80 mM 0.1135 mL 0.5677 mL 1.1355 mL 2.8386 mL
    100 mM 0.0908 mL 0.4542 mL 0.9084 mL 2.2709 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Sodium butyrate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Sodium butyrate156-54-7Butanoic acidButyric acidHDACAutophagyApoptosisEndogenous MetaboliteHistone deacetylasesanti-tumor activityneuroprotectionnasopharyngeal carcinoma5-8F6-10BapoptosisInhibitorinhibitorinhibit

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