Puromycin dihydrochloride (Synonyms: CL13900 dihydrochloride)

Name: Puromycin dihydrochloride
Brand: MedChemExpress
SKU: HY-B1743A
Rating: 5.0 (241 reviews)

Cat. No.: HY-B1743A Purity: 99.93%: Data Sheet
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COA
Handling Instructions
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Puromycin dihydrochloride (CL13900 dihydrochloride), an aminonucleoside antibiotic, inhibits protein synthesis.

For research use only. We do not sell to patients.

Size	Stock	Quantity

Free Sample (0.1 - 0.5 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote
5 g	Get quote

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Based on 241 publication(s) in Google Scholar

Other Forms of Puromycin dihydrochloride:

Puromycin-d₃ dihydrochloride In-stock
Puromycin In-stock
Puromycin (dihydrochloride) (Standard) Get quote

238 Publications Citing Use of MCE Puromycin dihydrochloride

: Puromycin dihydrochloride purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Jan 6. [Abstract]; Wild-type or mutated A549 cells subjected to the indicated treatments were stably transfected with puromycin dihydrochloride-inducible stubRFP-sensGFP-LC3 at an MOI of 5 (scale bar: 10 μm).

: Puromycin dihydrochloride purchased from MedChemExpress. Usage Cited in: Mol Cell. 2023 Nov 24:S1097-2765(23)00911-5. [Abstract]; Global protein synthesis analyzed by puromycin dihydrochloride (4 d) labeling assay upon METTL14 knockdown in HeLa cells. Silencing of EIF3A serves as a positive control.

: Puromycin dihydrochloride purchased from MedChemExpress. Usage Cited in: Mol Cell. 2023 Nov 24:S1097-2765(23)00911-5. [Abstract]; Global protein synthesis analyzed by puromycin dihydrochloride (4 d) labeling assay upon IGF2BP1-3 knockdown in HeLa cells.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Puromycin dihydrochloride (CL13900 dihydrochloride), an aminonucleoside antibiotic, inhibits protein synthesis^[1].

IC₅₀ & Target

Aminoglycoside

In Vitro

Puromycin blocks protein synthesis after aminoacyl-sRNA formation, and at the same time it leads to the accumulation of small peptides. Both of these effects appear to be due to the splitting of ribosome-bound peptidyl-sRNA,4 which results in release of incomplete peptide chains.^[1].
Puromycin, an analog of the 3' end of aminoacyl-tRNA, causes premature termination of translation by being linked non-specifically to growing polypeptide chains. Puromycin has two modes of inhibitory action. The first is by acting as an acceptor substrate which attacks peptidyl-tRNA in the P site to form a nascent peptide. The second is by competing with aminoacyl-tRNA for binding to the A' site^[2].
When used in minimal amounts, puromycin incorporation in neosynthesized proteins reflects directly the rate of mRNA translation in vitro. Puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling. It allows the direct evaluation of translation activity in single cells by immunofluorescence microscopy and in heterogenous populations of cells by fluorescenceactivated cell sorting^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

544.43

Formula

C₂₂H₃₁Cl₂N₇O₅

CAS No.

58-58-2

Appearance

Solid

Color

White to light yellow

SMILES

OC[C@@H]1[C@@H](NC([C@@H](N)CC2=CC=C(OC)C=C2)=O)[C@H]([C@H](N3C=NC4=C3N=CN=C4N(C)C)O1)O.[H]Cl.[H]Cl

Structure Classification

Initial Source

Microorganisms

Streptomyces alboniger

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

Methanol : 125 mg/mL (229.60 mM; Need ultrasonic)

H₂O : 50 mg/mL (91.84 mM; Need ultrasonic and warming)

DMSO : 50 mg/mL (91.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 5 mg/mL (9.18 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8368 mL	9.1839 mL	18.3678 mL
5 mM	0.3674 mL	1.8368 mL	3.6736 mL
10 mM	0.1837 mL	0.9184 mL	1.8368 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 4

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.5 mg/mL (0.92 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 5

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: 0.5 mg/mL (0.92 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 0.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (183.68 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.93%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (255 KB) HNMR (295 KB) RP-HPLC (262 KB) MS (68 KB) Elemental Analysis Report (113 KB)

Handling Instructions (2659 KB)

References

[1]. Nathans D, et al. Puromycin inhibition of protein synthesis: incorporation of puromycin intopeptide chains. Proc Natl Acad Sci U S A. 1964 Apr;51:585-92. [Content Brief]

[2]. Miyamoto-Sato E, et al. Specific bonding of puromycin to full-length protein at the C-terminus. Nucleic Acids Res. 2000 Mar 1;28(5):1176-82. [Content Brief]

[3]. Schmidt EK, et al. SUnSET, a nonradioactive method to monitor protein synthesis. Nat Methods. 2009 Apr;6(4):275-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / H₂O / DMSO / Methanol	1 mM	1.8368 mL	9.1839 mL	18.3678 mL	45.9196 mL
Ethanol / H₂O / DMSO / Methanol	5 mM	0.3674 mL	1.8368 mL	3.6736 mL	9.1839 mL
H₂O / DMSO / Methanol	10 mM	0.1837 mL	0.9184 mL	1.8368 mL	4.5920 mL
	15 mM	0.1225 mL	0.6123 mL	1.2245 mL	3.0613 mL
	20 mM	0.0918 mL	0.4592 mL	0.9184 mL	2.2960 mL
	25 mM	0.0735 mL	0.3674 mL	0.7347 mL	1.8368 mL
	30 mM	0.0612 mL	0.3061 mL	0.6123 mL	1.5307 mL
	40 mM	0.0459 mL	0.2296 mL	0.4592 mL	1.1480 mL
	50 mM	0.0367 mL	0.1837 mL	0.3674 mL	0.9184 mL
	60 mM	0.0306 mL	0.1531 mL	0.3061 mL	0.7653 mL
	80 mM	0.0230 mL	0.1148 mL	0.2296 mL	0.5740 mL
Methanol	100 mM	0.0184 mL	0.0918 mL	0.1837 mL	0.4592 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Puromycin dihydrochloride Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Puromycin58-58-2CL13900CL 13900CL-13900BacterialAntibioticParasiteInhibitorinhibitorinhibit

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Puromycin dihydrochloride (Synonyms: CL13900 dihydrochloride)

Customer Review

Other Forms of Puromycin dihydrochloride:

Puromycin dihydrochloride Related Antibodies

238 Publications Citing Use of MCE Puromycin dihydrochloride

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•Related Screening Libraries:

•Related Small Molecules:

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View All Antibiotic Isoform Specific Products:

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Biological Activity

Purity & Documentation

References

Customer Review

Puromycin dihydrochloride Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Puromycin dihydrochloride Related Classifications

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