2. Academic Validation
  3. High glucose mediates diabetic peripheral neuropathy by inducing Schwann cells apoptosis through the Dgkh/PKC-α signaling pathway

High glucose mediates diabetic peripheral neuropathy by inducing Schwann cells apoptosis through the Dgkh/PKC-α signaling pathway

  • Acta Diabetol. 2025 Jul 2. doi: 10.1007/s00592-025-02553-9.
Linhui Zuo 1,  Minli Qu 2,  Mengru Zhang 1,  Peng Cheng 1,  Min Guo 1,  Dinesh Selvarajah 3,  Solomon Tesfaye 3,  Jing Wu 4 5 6 7
Affiliations

Affiliations

  • 1 Department of Endocrinology, Xiangya Hospital, Central South University, Changsha, Hunan, China.
  • 2 Department of Hepatopathy and Endocrinology, The Affiliated Children's Hospital of Xiangya School of Medicine , Central South University (Hunan Children's Hospital), Changsha, Hunan, China.
  • 3 Diabetes Research Unit, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, UK.
  • 4 Department of Endocrinology, Xiangya Hospital, Central South University, Changsha, Hunan, China. wujing0731@csu.edu.cn.
  • 5 Hunan Engineering Research Center for Obesity and its Metabolic Complications, Xiangya Hospital, Central South University, Changsha, Hunan, China. wujing0731@csu.edu.cn.
  • 6 National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China. wujing0731@csu.edu.cn.
  • 7 Department of National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China. wujing0731@csu.edu.cn.
PMID: 40600984 DOI: 10.1007/s00592-025-02553-9
Abstract

Objective: Diabetic peripheral neuropathy (DPN) is one of the most common chronic complications of diabetes. The increased Apoptosis of Schwann cells (SCs) induced by high glucose (HG) is significant in the pathogenesis of DPN, but the mechanism remains unclear. Diacylglycerol kinase eta (Dgkh) is a member of the diacylglycerol kinases (DGKs) family that participates in glucose uptake, utilization, and energy homeostasis. But its role in DPN has not been reported.

Methods: Streptozotocin (STZ)-induced SD rats were used as an animal model of DPN and human Schwann cells (HSCs) were used as an in vitro model of simulated HG conditions. Behavioral tests, histopathology, the mRNA and protein expression levels were detected in vivo. Further, Dgkh was knocked down in vitro, and PKC-α agonist PMA and inhibitor Ro 31-8220 were added to HSCs to observe the effect of Dgkh/PKC-α on HSCs Apoptosis.

Results: The mechanical and thermal pain thresholds were significantly decreased in DPN rats induced by STZ. The increased Apoptosis of the sciatic nerve in STZ-induced DPN rats is accompanied by the upregulation of Dgkh expression. HG leads to increased HSCs Apoptosis by Dgkh increased expression. Meanwhile, the knockdown of Dgkh significantly improved HSCs Apoptosis induced by HG. PMA effectively improved Apoptosis in HG-induced HSCs, but did not affect Dgkh expression. And we discovered that the Apoptosis of HSCs reversed by Dgkh knockdown vanished when the PKC-α inhibitor Ro 31-8220 was added.

Conclusion: Dgkh expression increased under HG conditions and triggered Apoptosis of HSCs, boosting DPN via inhibiting PKC-α.

Keywords

Apoptosis; Dgkh; Diabetic peripheral neuropathy; High glucose; Schwann cells.

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