2. Metabolic Enzyme/Protease Apoptosis Cell Cycle/DNA Damage
  3. Endogenous Metabolite Apoptosis DNA/RNA Synthesis
  4. L-Asparagine

L-Asparagine  (Synonyms: (-)-Asparagine; Asn; Asparamide)

Cat. No.: HY-N0667 Purity: ≥98.0%
SDS
COA
Handling Instructions Technical Support

L-Asparagine is an essential amino acid for leukemic cells and a substrate for L-Asparaginase. L-Asparaginase is a potent anti-leukemic enzyme that promotes asparagine (Asn) and glutamine (Gln) depletion and inhibits protein biosynthesis in lymphoblasts. Removal of L-asparagine from plasma by L-Asparaginase results in inhibition of RNA and DNA synthesis and subsequent apoptosis. L-Asparaginase has cell-killing ability in vitro and in vivo, and selectively inhibits the growth of cancer cells with low asparagine synthetase (AASNS) expression. L-Asparagine can be used as a biomarker and sensor for the study of childhood acute lymphoblastic leukemia.

For research use only. We do not sell to patients.

L-Asparagine Chemical Structure

L-Asparagine Chemical Structure

CAS No. : 70-47-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in Water In-stock
Solid
100 mg In-stock
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Based on 3 publication(s) in Google Scholar

Other Forms of L-Asparagine:

L-Asparagine Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

L-Asparagine is an essential amino acid for leukemic cells and a substrate for L-Asparaginase. L-Asparaginase is a potent anti-leukemic enzyme that promotes asparagine (Asn) and glutamine (Gln) depletion and inhibits protein biosynthesis in lymphoblasts. Removal of L-asparagine from plasma by L-Asparaginase results in inhibition of RNA and DNA synthesis and subsequent apoptosis. L-Asparaginase has cell-killing ability in vitro and in vivo, and selectively inhibits the growth of cancer cells with low asparagine synthetase (AASNS) expression. L-Asparagine can be used as a biomarker and sensor for the study of childhood acute lymphoblastic leukemia[1][2][3][4][5].

IC50 & Target

Microbial Metabolite

 

Human Endogenous Metabolite

 

In Vitro

L-Asparagine is the biological substrate of L-Asparaginase. The potential side effects of L-Asparaginase are causing normal cell death and inducing inflammation, metabolic disorders and neurotoxicity after use. The toxic side effects of L-Asparaginase may be caused by the action of glutaminase in it[1].
L-Asparagine and L-Asparaginase have also been used to develop biosensors for analyzing asparagine levels in leukemia or food industry. In application, the characteristics of L-Asparagine hydrolysis, which produces ammonium ions and causes pH changes, can be used to determine the level of L-Asparagine by color and absorbance changes[1].
L-Asparaginase can preferentially kill leukemia cells, and the leukemia cell killing rate can be accurately measured using leukemia cell surface markers. L-Asparaginase showed no significant dose response at doses of 0.0001-0.1 IU/mL[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

L-Asparagine Related Antibodies
In Vivo

L-Asparagine is widely found in bacteria, fungi, actinomycetes, plants and animals. L-Asparagine from Escherichia coli (E. coli) and Enterobacter chrysanthemi (E. chrysanthemi) has been used to acute lymphoblastic leukemia (ALL) research, and has made progress in the subcutaneous Gardner 6C3HED lymphosarcoma model established in mice[1].
In the NOD.Cg-PRKDCscidIL2RGtm1Wjl (NSG) mouse model, L-Asparaginase (1000 or 5000 IU/kg; intraperitoneal injection; once a day for 3 consecutive days) significantly depletes L-Asparagine in the blood; in the high-dose group, L-Asparagine also recoveres slowly after the cessation of L-Asparaginase injection[3].
When L-Asparaginase is encapsulated in red blood cells (ASP-RBC) and given to Swiss mice by intravenous injection (200 or 1000 IU/kg), the depletion time of L-Asparagine in their plasma is prolonged. In the 1000 IU/kg dose group, levels of L-Asparagine remains undetectable for up to 14 days, and the half-life of the enzyme is 9-10 days[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

132.12

Formula

C4H8N2O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

N[C@H](C(O)=O)CC(N)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

H2O : 6.67 mg/mL (50.48 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 7.5689 mL 37.8444 mL 75.6888 mL
5 mM 1.5138 mL 7.5689 mL 15.1378 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 10 mg/mL (75.69 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

SDS (393 KB)

COA (245 KB) HNMR (228 KB) Elemental Analysis Report (103 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 7.5689 mL 37.8444 mL 75.6888 mL 189.2219 mL
5 mM 1.5138 mL 7.5689 mL 15.1378 mL 37.8444 mL
10 mM 0.7569 mL 3.7844 mL 7.5689 mL 18.9222 mL
15 mM 0.5046 mL 2.5230 mL 5.0459 mL 12.6148 mL
20 mM 0.3784 mL 1.8922 mL 3.7844 mL 9.4611 mL
25 mM 0.3028 mL 1.5138 mL 3.0276 mL 7.5689 mL
30 mM 0.2523 mL 1.2615 mL 2.5230 mL 6.3074 mL
40 mM 0.1892 mL 0.9461 mL 1.8922 mL 4.7305 mL
50 mM 0.1514 mL 0.7569 mL 1.5138 mL 3.7844 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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L-Asparagine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-Asparagine70-47-3(-)-Asparagine Asn AsparamideEndogenous MetaboliteApoptosisDNA/RNA SynthesisAsparagine IPEC-J2AntitumorODCInhibitorinhibitorinhibit

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