2. Anti-infection Apoptosis MAPK/ERK Pathway NF-κB PI3K/Akt/mTOR Immunology/Inflammation
  3. Bacterial Apoptosis p38 MAPK NF-κB MAPKAPK2 (MK2) Akt NO Synthase Interleukin Related Caspase
  4. Dehydrocostus Lactone

Dehydrocostus Lactone  (Synonyms: (-)-Dehydrocostus lactone; Epiligulyl oxide)

Cat. No.: HY-N0591 Purity: 99.95%
SDS
COA
Handling Instructions Technical Support

Dehydrocostus Lactone ((-)-Dehydrocostus lactone) is a natural sesquiterpene that can be isolated from Saussurea lappa. Dehydrocostus Lactone has multiple activities such as anti-inflammatory, antibacterial, anti-tumor, and immunomodulatory effects. Dehydrocostus Lactone has an MIC of 2 µg/mL against Mycobacterium tuberculosis. Dehydrocostus Lactone can also inhibit the killing activity of cytotoxic T lymphocytes and induce apoptosis in tumor cells.

For research use only. We do not sell to patients.

Dehydrocostus Lactone Chemical Structure

Dehydrocostus Lactone Chemical Structure

CAS No. : 477-43-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Dehydrocostus Lactone:

Dehydrocostus Lactone Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Dehydrocostus Lactone

View All p38 MAPK Isoform Specific Products:

View All Isoforms
p38 MAPK p38α p38β MAPK13 p38δ p38γ

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

View All Akt Isoform Specific Products:

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Akt Akt1 Akt2 Akt3

View All NO Synthase Isoform Specific Products:

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nNOS iNOS eNOS

View All Interleukin Related Isoform Specific Products:

View All Isoforms
IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

View All Caspase Isoform Specific Products:

View All Isoforms
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Dehydrocostus Lactone ((-)-Dehydrocostus lactone) is a natural sesquiterpene that can be isolated from Saussurea lappa. Dehydrocostus Lactone has multiple activities such as anti-inflammatory, antibacterial, anti-tumor, and immunomodulatory effects. Dehydrocostus Lactone has an MIC of 2 µg/mL against Mycobacterium tuberculosis. Dehydrocostus Lactone can also inhibit the killing activity of cytotoxic T lymphocytes and induce apoptosis in tumor cells[1][2][3][4].

Cellular Effect
Cell Line Type Value Description References
HL-60 IC50
3.7 μM
Compound: Dehydrocostus lactone
Cytotoxicity against human HL60 cells after 72 hrs by MTT assay
Cytotoxicity against human HL60 cells after 72 hrs by MTT assay
[PMID: 26832219]
HL-60 IC50
5.3 μM
Compound: 1
Cytotoxicity against human HL60 cells after 18 hrs by annexin-V labeling-based flow cytometry
Cytotoxicity against human HL60 cells after 18 hrs by annexin-V labeling-based flow cytometry
[PMID: 22985027]
HL60/ADR IC50
5.1 μM
Compound: Dehydrocostus lactone
Cytotoxicity against human HL60/ADR cells after 72 hrs by MTT assay
Cytotoxicity against human HL60/ADR cells after 72 hrs by MTT assay
[PMID: 26832219]
K562 IC50
5.3 μM
Compound: Dehydrocostus lactone
Cytotoxicity against human K562 cells after 72 hrs by MTT assay
Cytotoxicity against human K562 cells after 72 hrs by MTT assay
[PMID: 26832219]
K562/A02 IC50
6.6 μM
Compound: Dehydrocostus lactone
Cytotoxicity against human K562/A02 cells after 72 hrs by MTT assay
Cytotoxicity against human K562/A02 cells after 72 hrs by MTT assay
[PMID: 26832219]
KG-1a IC50
10.5 μM
Compound: Dehydrocostus lactone
Cytotoxicity against human KG1a cells after 72 hrs by MTT assay
Cytotoxicity against human KG1a cells after 72 hrs by MTT assay
[PMID: 26832219]
In Vitro

Dehydrocostus lactone (0-2 mg/L; 48 h) can reduce the viability, induce apoptosis, inhibit the expression of anti-apoptotic proteins, and increase the expression of pro-apoptotic proteins in DU145 cells[1].
Dehydrocostus lactone (0-30 μmol/L; 0.5-8.5 h) can inhibit the phosphorylation of p38 MAPK, MK2, and Akt, the activation of NF-κB, reduce the expression of iNOS and the production of NO, and decrease the mRNA levels of pro-inflammatory cytokines such as TNF-α, IL-6, IL-1β, and IL-12 in RAW264.7 cells treated with LPS (HY-D1056)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Dehydrocostus Lactone Related Antibodies

Western Blot Analysis[1]

Cell Line: DU145 human prostate cancer cells
Concentration: 2 mg/L
Incubation Time: 48 h
Result: Decreased the level of anti-apoptotic protein Bcl-xL.
Increased the levels of pro-apoptotic proteins Bax, Bak, Bok, Bik, Bmf, and t-Bid.
Increased the levels of cleaved caspase-8, -9, -7, -3, and cleaved PARP.

Cell Viability Assay[2]

Cell Line: LPS (HY-D1056)-stimulated RAW264.7 cells.
Concentration: 0, 3, 5, 10 and 30 µmol/L
Incubation Time: Pretreated with 0.5 h, then co-incubation with LPS (HY-D1056) for 0, 0.5, 1, 2, 4 and 8 h
Result: Inhibited the protein expression levels of p-p38 MAPK, p-MK2, p-Akt.
In Vivo

Dehydrocostus Lactone (5-20 mg/kg; intraperitoneal injection; single administration) has a protective effect in a mouse model of LPS (HY-D1056)-induced acute lung injury[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice (male, 6-8 weeks old) treated LPS[2]
Dosage: 5, 10 and 20 mg/kg
Administration: Intraperitoneal injection (i.p.); single dose
Result: Effectively reduced the LPS-stimulated infiltration of inflammatory cells and thickening of the alveolar walls.
Alleviated LPS-induced lung injury scores significantly.
Decreased the cell counts and total protein concentration in BALF.
Reduced the increased MPO activity induced by LPS challenge.
Significantly reduced the mRNA expression levels of iNOS, TNF-α, IL-6, IL-12 and IL-1β in lung tissues.
Molecular Weight

230.30

Formula

C15H18O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(O[C@@]([C@@]1([H])C(CC[C@@]21[H])=C)([H])[C@@]3([H])CCC2=C)C3=C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (1085.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 15 mg/mL (65.13 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.3422 mL 21.7108 mL 43.4216 mL
5 mM 0.8684 mL 4.3422 mL 8.6843 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (9.03 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (9.03 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.97%

SDS (393 KB)

COA (260 KB) HNMR (215 KB) LCMS (332 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 4.3422 mL 21.7108 mL 43.4216 mL 108.5541 mL
5 mM 0.8684 mL 4.3422 mL 8.6843 mL 21.7108 mL
10 mM 0.4342 mL 2.1711 mL 4.3422 mL 10.8554 mL
15 mM 0.2895 mL 1.4474 mL 2.8948 mL 7.2369 mL
20 mM 0.2171 mL 1.0855 mL 2.1711 mL 5.4277 mL
25 mM 0.1737 mL 0.8684 mL 1.7369 mL 4.3422 mL
30 mM 0.1447 mL 0.7237 mL 1.4474 mL 3.6185 mL
40 mM 0.1086 mL 0.5428 mL 1.0855 mL 2.7139 mL
50 mM 0.0868 mL 0.4342 mL 0.8684 mL 2.1711 mL
60 mM 0.0724 mL 0.3618 mL 0.7237 mL 1.8092 mL
DMSO 80 mM 0.0543 mL 0.2714 mL 0.5428 mL 1.3569 mL
100 mM 0.0434 mL 0.2171 mL 0.4342 mL 1.0855 mL
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Dehydrocostus Lactone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dehydrocostus Lactone477-43-0(-)-Dehydrocostus lactone Epiligulyl oxideBacterialApoptosisp38 MAPKNF-κBMAPKAPK2 (MK2)AktNO SynthaseInterleukin RelatedCaspaseNuclear factor-κBNuclear factor-kappaBMitogen-activated protein kinase activated protein kinase 2MAP kinase activated protein kinase 2MAPK activated protein kinase 2MAPKAP kinase 2PKBProtein kinase BNitric oxide synthasesNOSILDU145 cellsRAW264.7 cellsacute lung injury modelInhibitorinhibitorinhibit

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