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  4. 6-AF488 tyramide

6-AF488 tyramide is a bright, green fluorescent dye (Ex=496 nm, Em=524 nm). 6-AF488 tyramide is utilized as reporter fluorescent substrate of horseradish peroxidase (HRP)-catalyzed deposition for tyramide signal amplification (TSA). 5-FITC tyramide can be used for multiplex immunohistochemistry (mIHC).

For research use only. We do not sell to patients.

6-AF488 tyramide

6-AF488 tyramide Chemical Structure

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6-AF488 tyramide Related Antibodies

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Description

6-AF488 tyramide is a bright, green fluorescent dye (Ex=496 nm, Em=524 nm). 6-AF488 tyramide is utilized as reporter fluorescent substrate of horseradish peroxidase (HRP)-catalyzed deposition for tyramide signal amplification (TSA). 5-FITC tyramide can be used for multiplex immunohistochemistry (mIHC)[1].

In Vitro

6-AF488 tyramide assay[1].
a. Place tissues in per well in a 24-well plate, and it’s important to not let tissues sections dry.
b. Permeabilize tissues with 0.2 % Triton X-100 in PBS for 5-10 min.
c. Rinse with PBS-T for 5-10 min, and then incubate tissues in 1 % H2O2 for 20 min to block endogenous peroxidase activity.
d. Rinse with PBS three times, 5-10 min each, and then incubate in 1 % blocking solution (4 % normal donkey serum + PBS-T) in PBS for 1 h for to block nonspecific binding sites.
e. Apply the primary antibody working solution for 2 h at RT or overnight at 4 °C, and then rinse with PBS three times, 5-10 min each.
f. Apply the HRP-conjugated secondary goat anti rabbit antibody (1:100 in 1 % blocking solution) for 2 h at RT , and then rinse with PBS three times, 5-10 min each.
g. Activate TSA buffer with 0.0015 % H2O2, and then incubate tissues in 6-AF488 tyramide working solution (1: 100 in the activated TSA buffer) for 5-10 min at RT.
h. Rinse with PBS three times, 5-10 min each, and then mount sections on slides.
i. Place in slide holder in a nearly vertical position for 10 min to dry slides, and then briefly rinse slides with ddH2O following drying for 10 min.
j. Mount slides with mounting medium, we recommend using AntiFade Mounting Medium (with DAPI) (HY-K1047).
k. Fluorescence microscopy detection.
Note: it is suggested that the optimal concentration of 6-AF488 tyramide working solution according to actual conditions.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

6-AF488 tyramide Related Antibodies
Molecular Weight

653.64

Formula

C29H23N3O11S2
SMILES

O=C(O)C(C=C(C=C1)C(NCCC2=CC=C(C=C2)O)=O)=C1C(C3=C4C(S(=O)(O)=O)=C(N)C=C3)=C5C=CC(C(S(=O)([O-])=O)=C5O4)=[NH2+]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (152.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5299 mL 7.6495 mL 15.2989 mL
5 mM 0.3060 mL 1.5299 mL 3.0598 mL
View the Complete Stock Solution Preparation Table

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5299 mL 7.6495 mL 15.2989 mL 38.2474 mL
5 mM 0.3060 mL 1.5299 mL 3.0598 mL 7.6495 mL
10 mM 0.1530 mL 0.7649 mL 1.5299 mL 3.8247 mL
15 mM 0.1020 mL 0.5100 mL 1.0199 mL 2.5498 mL
20 mM 0.0765 mL 0.3825 mL 0.7649 mL 1.9124 mL
25 mM 0.0612 mL 0.3060 mL 0.6120 mL 1.5299 mL
30 mM 0.0510 mL 0.2550 mL 0.5100 mL 1.2749 mL
40 mM 0.0382 mL 0.1912 mL 0.3825 mL 0.9562 mL
50 mM 0.0306 mL 0.1530 mL 0.3060 mL 0.7649 mL
60 mM 0.0255 mL 0.1275 mL 0.2550 mL 0.6375 mL
80 mM 0.0191 mL 0.0956 mL 0.1912 mL 0.4781 mL
100 mM 0.0153 mL 0.0765 mL 0.1530 mL 0.3825 mL
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6-AF488 tyramide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

6-AF488 tyramideFluorescent DyeFluorescent dyeTSAGreenMultiplex immunohistochemistry (mIHC)Inhibitorinhibitorinhibit

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