3-Hydroxyphenylacetic acid

Name: 3-Hydroxyphenylacetic acid
Brand: MedChemExpress
SKU: HY-W001083
Rating: 4.5 (1 reviews)

Cat. No.: HY-W001083 Purity: 99.03%: Data Sheet
COA

SDS
Handling Instructions
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3-Hydroxyphenylacetic acid is an orally active flavonoid metabolite produced by intestinal flora, with blood pressure-lowering activity. 3-Hydroxyphenylacetic acid can also inhibit ferroptosis by upregulating the expression of GPX4, thereby improving spermatogenic dysfunction in aged mice. In addition, abnormal levels of 3-Hydroxyphenylacetic acid are closely related to certain diseases, such as autism spectrum disorders.

For research use only. We do not sell to patients.

3-Hydroxyphenylacetic acid Chemical Structure

CAS No. : 621-37-4

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote
5 g	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 3-Hydroxyphenylacetic acid:

3-Hydroxyphenylacetic acid (Standard) Get quote

1 Publications Citing Use of MCE 3-Hydroxyphenylacetic acid

•Microbiome. 2023 Sep 27;11(1):212. [Abstract]

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ALDH1 ALDH2 ALDH3

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

3-Hydroxyphenylacetic acid (0-50 μM; 6-9 h) can enhance total ALDH activity and completely inhibit acetaldehyde-induced cytotoxicity in mouse hepatocellular carcinoma Hepa1c1c7 cells^[1].
3-Hydroxyphenylacetic acid (0.1-1 mM) can induce dose-dependent vasodilation in porcine coronary artery segments^[2].
3-Hydroxyphenylacetic acid (50 μM; 48 h) can inhibit cell apoptosis, reduce ROS production, decrease MDA levels, increase GPx activity, and upregulate GPX4 protein expression in H₂O₂-induced senescent GC-2 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:	GC-2 cells treated H₂O₂
Concentration:	50 μM
Incubation Time:	48 h
Result:	Significantly increased GPX4 protein expression, although no change was found for FTL, ACSL4, and xCT.

In Vivo

3-Hydroxyphenylacetic acid (0.001-10 mg/kg; intravenous bolus; single dose) can reduce arterial blood pressure in spontaneously hypertensive rats^[2].
3-Hydroxyphenylacetic acid (25 mg/kg; oral gavage; 6 weeks) can improve spermatogenic dysfunction in aged mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Spontaneously hypertensive rats (SHR) (average weight 356 ± 21 g)^[2]
Dosage:	0.001, 0.01. 0.1, 0.5, 1, 2.5, 5 and 10 mg/kg (the volume applied was always 0.1 mL)
Administration:	Intravenous bolus; single dose
Result:	Decreased the systolic, diastolic, and mean arterial pressures of SHR in a dose-dependent manner, with 0.01 mg/kg significantly reducing diastolic pressure and 10 mg/kg showing the most significant antihypertensive effect. Had no significant effect on heart rate.

Animal Model:	Male C57BL/6J mice aged 18-20 months old^[3]
Dosage:	25 mg/kg
Administration:	Oral gavage; 6 weeks
Result:	Increased sperm concentration. Improved spermatogenesis with better-organized seminiferous tubules and increased numbers of spermatogonia, spermatocytes, spermatids, and spermatozoa. Increased the expression of GPX4 and NRF2. Increased total GPx activity, level of GSH, and ratio of GSH/GSSG, along with decreased MDA abundance, indicating restrained ferroptosis.

Molecular Weight

152.15

Formula

C₈H₈O₃

CAS No.

621-37-4

Appearance

Solid

Color

White to off-white

SMILES

OC1=CC=CC(CC(O)=O)=C1

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (328.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 25 mg/mL (164.31 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.5725 mL	32.8623 mL	65.7246 mL
5 mM	1.3145 mL	6.5725 mL	13.1449 mL
10 mM	0.6572 mL	3.2862 mL	6.5725 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (16.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (16.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (16.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.03%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (248 KB) LCMS (90 KB)

Handling Instructions (2659 KB)

References

[1]. Liu Y, et al. A Major Intestinal Catabolite of Quercetin Glycosides, 3-Hydroxyphenylacetic Acid, Protects the Hepatocytes from the Acetaldehyde-Induced Cytotoxicity through the Enhancement of the Total Aldehyde Dehydrogenase Activity. Int J Mol Sci. 2022 Feb 3;23(3):1762. [Content Brief]

[2]. Dias P, et al. 3-Hydroxyphenylacetic Acid: A Blood Pressure-Reducing Flavonoid Metabolite. Nutrients. 2022 Jan 13;14(2):328. [Content Brief]

[3]. Jin Z, et al. The gut metabolite 3-hydroxyphenylacetic acid rejuvenates spermatogenic dysfunction in aged mice through GPX4-mediated ferroptosis. Microbiome. 2023 Sep 27;11(1):212. [Content Brief]

[4]. Xiong X, et al. Urinary 3-(3-Hydroxyphenyl)-3-hydroxypropionic Acid, 3-Hydroxyphenylacetic Acid, and 3-Hydroxyhippuric Acid Are Elevated in Children with Autism Spectrum Disorders. Biomed Res Int. 2016;2016:9485412. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	6.5725 mL	32.8623 mL	65.7246 mL	164.3115 mL
	5 mM	1.3145 mL	6.5725 mL	13.1449 mL	32.8623 mL
	10 mM	0.6572 mL	3.2862 mL	6.5725 mL	16.4312 mL
	15 mM	0.4382 mL	2.1908 mL	4.3816 mL	10.9541 mL
	20 mM	0.3286 mL	1.6431 mL	3.2862 mL	8.2156 mL
	25 mM	0.2629 mL	1.3145 mL	2.6290 mL	6.5725 mL
	30 mM	0.2191 mL	1.0954 mL	2.1908 mL	5.4771 mL
	40 mM	0.1643 mL	0.8216 mL	1.6431 mL	4.1078 mL
	50 mM	0.1314 mL	0.6572 mL	1.3145 mL	3.2862 mL
	60 mM	0.1095 mL	0.5477 mL	1.0954 mL	2.7385 mL
	80 mM	0.0822 mL	0.4108 mL	0.8216 mL	2.0539 mL
	100 mM	0.0657 mL	0.3286 mL	0.6572 mL	1.6431 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.