α-Casein (90-95) TFA

Name: α-Casein (90-95) TFA
Brand: MedChemExpress
SKU: HY-P1793A
Rating: 4.5 (1 reviews)

Cat. No.: HY-P1793A Purity: 99.58%: Data Sheet
SDS

COA
Handling Instructions
FAQs
Technical Support

α-Casein (90-95) TFA is a partial agonist of opioid receptors and a copper ion ligand, with opioid activity. α-Casein (90-95) TFA inhibits the secretion of β-hexosaminidase by rat peritoneal mast cells (PMC) with IC₅₀= 0.1 μM. α-Casein (90-95) TFA inhibits the proliferation of prostate cancer cells LNCaP, DU145, and PC3 with IC₅₀ of 0.94 nM, 137 nM, and 6.92 nM, respectively. α-Casein (90-95) TFA activates G_i-like proteins through a membrane-assisted, receptor-independent pathway, or reversibly binds to opioid receptors, inducing intracellular calcium release and conformational changes, and exerts the activity of promoting mast cell secretion and inhibiting tumor cell proliferation. α-Casein (90-95) TFA can be used in the study of the mechanisms of allergic diseases and prostate cancer.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

α-Casein (90-95) TFA Chemical Structure

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of α-Casein (90-95) TFA:

α-Casein (90-95) In-stock

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•Related Small Molecules:

•Related Proteins:

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μ Opioid Receptor/MOR κ Opioid Receptor/KOR δ Opioid Receptor/DOR NOP Receptor/ORL1

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

α-Casein (90-95) TFA (0.1 μM; 10 min) can significantly induce the secretion of β-hexosaminidase from rat peritoneal mast cells (PMC)^[2].
α-Casein (90-95) TFA (0.94 nM-137 nM; 4-6 days) inhibits the proliferation of prostate cancer cells (LNCaP, DU145, PC3) in a dose-dependent manner, with an inhibition rate of 30%-59%^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA Assay^[2]

Cell Line:	Rat peritoneal mast cells (PMC)
Concentration:	0.1 mM (secretagogue assay), 0.5 mM (BAC inhibition assay)
Incubation Time:	10 min (secretion), 5 min (BAC preincubation)
Result:	Induced 15.2% β-hexosaminidase release (vs. 5.5% spontaneous release) at 0.1 mM, with maximal secretion within 1 min. Preincubation with 3.0 μg/mL Benzalkonium chloride (HY-B2232) abolished secretion (inhibition 50.1%), indicating membrane-mediated G_i-protein activation.

Molecular Weight

897.94

Formula

C₄₀H₅₈F₃N₉O₁₁

Appearance

Solid

Color

White to off-white

Sequence

Arg-Tyr-Leu-Gly-Tyr-Leu

Sequence Shortening

RYLGYL

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light, under nitrogen

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (111.37 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1137 mL	5.5683 mL	11.1366 mL
5 mM	0.2227 mL	1.1137 mL	2.2273 mL
10 mM	0.1114 mL	0.5568 mL	1.1137 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.58%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (245 KB) RP-HPLC (111 KB) MS (199 KB) Elemental Analysis Report (105 KB)

Handling Instructions (2659 KB)

References

[1]. Chruścińska E, et al. Binding ability of Cu2+ ions by opiate-like fragments of bovine casein. J Inorg Biochem. 1997 Apr;66(1):19-22. [Content Brief]

[2]. Stępnik M, et al. The influence of bovine casein-derived exorphins on mast cells in rodents[J]. Revue française d'allergologie et d'immunologie clinique, 2002, 42(5): 447-453.

[3]. Kampa M, et al. Opioid alkaloids and casomorphin peptides decrease the proliferation of prostatic cancer cell lines (LNCaP, PC3 and DU145) through a partial interaction with opioid receptors. Eur J Pharmacol. 1997 Sep 24;335(2-3):255-65. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.1137 mL	5.5683 mL	11.1366 mL	27.8415 mL
	5 mM	0.2227 mL	1.1137 mL	2.2273 mL	5.5683 mL
	10 mM	0.1114 mL	0.5568 mL	1.1137 mL	2.7842 mL
	15 mM	0.0742 mL	0.3712 mL	0.7424 mL	1.8561 mL
	20 mM	0.0557 mL	0.2784 mL	0.5568 mL	1.3921 mL
	25 mM	0.0445 mL	0.2227 mL	0.4455 mL	1.1137 mL
	30 mM	0.0371 mL	0.1856 mL	0.3712 mL	0.9281 mL
	40 mM	0.0278 mL	0.1392 mL	0.2784 mL	0.6960 mL
	50 mM	0.0223 mL	0.1114 mL	0.2227 mL	0.5568 mL
	60 mM	0.0186 mL	0.0928 mL	0.1856 mL	0.4640 mL
	80 mM	0.0139 mL	0.0696 mL	0.1392 mL	0.3480 mL
	100 mM	0.0111 mL	0.0557 mL	0.1114 mL	0.2784 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.