2. GPCR/G Protein Neuronal Signaling Others
  3. Opioid Receptor Biochemical Assay Reagents
  4. α-Casein (90-95)

α-Casein (90-95) is a partial agonist of opioid receptors and a copper ion ligand, with opioid activity. α-Casein (90-95) inhibits the secretion of β-hexosaminidase by rat peritoneal mast cells (PMC) with IC50= 0.1 μM. α-Casein (90-95) inhibits the proliferation of prostate cancer cells LNCaP, DU145, and PC3 with IC50 of 0.94 nM, 137 nM, and 6.92 nM, respectively. α-Casein (90-95) activates Gi-like proteins through a membrane-assisted, receptor-independent pathway, or reversibly binds to opioid receptors, inducing intracellular calcium release and conformational changes, and exerts the activity of promoting mast cell secretion and inhibiting tumor cell proliferation. α-Casein (90-95) can be used in the study of the mechanisms of allergic diseases and prostate cancer.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

α-Casein (90-95) Chemical Structure

α-Casein (90-95) Chemical Structure

CAS No. : 83471-50-5

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Based on 1 publication(s) in Google Scholar

Other Forms of α-Casein (90-95):

α-Casein (90-95) Related Antibodies

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Description

α-Casein (90-95) is a partial agonist of opioid receptors and a copper ion ligand, with opioid activity. α-Casein (90-95) inhibits the secretion of β-hexosaminidase by rat peritoneal mast cells (PMC) with IC50= 0.1 μM. α-Casein (90-95) inhibits the proliferation of prostate cancer cells LNCaP, DU145, and PC3 with IC50 of 0.94 nM, 137 nM, and 6.92 nM, respectively. α-Casein (90-95) activates Gi-like proteins through a membrane-assisted, receptor-independent pathway, or reversibly binds to opioid receptors, inducing intracellular calcium release and conformational changes, and exerts the activity of promoting mast cell secretion and inhibiting tumor cell proliferation. α-Casein (90-95) can be used in the study of the mechanisms of allergic diseases and prostate cancer[1][2][3].

In Vitro

α-Casein (90-95) (0.1 μM; 10 min) can significantly induce the secretion of β-hexosaminidase from rat peritoneal mast cells (PMC)[2].
α-Casein (90-95) (0.94 nM-137 nM; 4-6 days) inhibits the proliferation of prostate cancer cells (LNCaP, DU145, PC3) in a dose-dependent manner, with an inhibition rate of 30%-59%[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

α-Casein (90-95) Related Antibodies

ELISA Assay[2]

Cell Line: Rat peritoneal mast cells (PMC)
Concentration: 0.1 mM (secretagogue assay), 0.5 mM (BAC inhibition assay)
Incubation Time: 10 min (secretion), 5 min (BAC preincubation)
Result: Induced 15.2% β-hexosaminidase release (vs. 5.5% spontaneous release) at 0.1 mM, with maximal secretion within 1 min.
Preincubation with 3.0 μg/mL Benzalkonium chloride (HY-B2232) abolished secretion (inhibition 50.1%), indicating membrane-mediated Gi-protein activation.
Molecular Weight

783.91

Formula

C38H57N9O9
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Arg-Tyr-Leu-Gly-Tyr-Leu
Sequence Shortening

RYLGYL
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
Purity & Documentation
References
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α-Casein (90-95) Related Classifications

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  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

α-Casein (90-95)83471-50-5Opioid ReceptorBiochemical Assay ReagentsInhibitorinhibitorinhibit

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