2. Cell Cycle/DNA Damage Cytoskeleton PI3K/Akt/mTOR Apoptosis
  3. Microtubule/Tubulin PI3K Akt Apoptosis
  4. Tubulin-IN-55

Tubulin-IN-55 is a tubulin inhibitor. Tubulin-IN-55 disrupts the PI3K/Akt signaling pathway in cancer cells. Tubulin-IN-55 exerts broad-spectrum anti-proliferative activity against multiple tumor cells (HeLa, HCT116, 4T1, A549, H1299, MDA-MB231). Tubulin-IN-55 induces G2/M phase arrest and apoptosis, and inhibits tumor cell migration/invasion in cancer cells. Tubulin-IN-55 demonstrates potent antitumor efficacy in orthotopic autologous transplantation mice. Tubulin-IN-55 can be used for the study of cancer.

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Tubulin-IN-55

Tubulin-IN-55 Chemical Structure

CAS No. : 353258-94-3

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Tubulin-IN-55 Related Antibodies

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PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

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Description

Tubulin-IN-55 is a tubulin inhibitor. Tubulin-IN-55 disrupts the PI3K/Akt signaling pathway in cancer cells. Tubulin-IN-55 exerts broad-spectrum anti-proliferative activity against multiple tumor cells (HeLa, HCT116, 4T1, A549, H1299, MDA-MB231). Tubulin-IN-55 induces G2/M phase arrest and apoptosis, and inhibits tumor cell migration/invasion in cancer cells. Tubulin-IN-55 demonstrates potent antitumor efficacy in orthotopic autologous transplantation mice. Tubulin-IN-55 can be used for the study of cancer[1].

In Vitro

Tubulin-IN-55 (Compound 89) (0.1-10 μM, 24 h) shows potent antiproliferative activity against HeLa, HCT116, and 4T1 cells, with IC50 values of 1.20 μM, 4.91 μM, 1.13 μM, and 0.65 μM , respectively[1].
Tubulin-IN-55 (0.3-0.9 μM, 12-24 h) exhibits anti-migratory and anti-invasive effects on HeLa, HCT116, and 4T1 cells[1].
Tubulin-IN-55 (0.3-1.2 μM, 24 h) induces apoptosis and G2/M phase arrest in HeLa, HCT116, and 4T1 cells[1].
Tubulin-IN-55 (6.25-100 μM, 60 min) inhibits tubulin polymerization in vitro in a dose-dependent manner[1].
Tubulin-IN-55 (0.3-0.9 μM, 24 h) downregulates the phosphorylation levels of PI3K and Akt in HeLa, HCT116, and 4T1 cells without changing total PI3K and Akt levels[1].
Tubulin-IN-55 (0.1-10 μM, 24 h) exerts antitumor activity on BC-PDO1, BC-PDO2, and BC-PDO10 (breast cancer organoids), reducing organoid viability with IC50 values of 1.07 μM, 0.81 μM, and 0.42 μM, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tubulin-IN-55 Related Antibodies

Cell Migration Assay [1]

Cell Line: HeLa, HCT116, and 4T1 cells
Concentration: 0.3, 0.9 μM
Incubation Time: 12, 24 h
Result: Exhibited anti-migratory effects on HeLa, HCT116, and 4T1 cells.
Upregulated epithelial marker E-Cadherin.

Cell Invasion Assay[1]

Cell Line: HeLa, HCT116, and 4T1 cells
Concentration: 0.3, 0.9 μM
Incubation Time: 12, 24 h
Result: Exhibited anti-migratory effects on HeLa, HCT116, and 4T1 cells.
Upregulated epithelial marker E-Cadherin.

Apoptosis Analysis[1]

Cell Line: 0.3, 0.6, 1.2 μM
Concentration: 0.3, 0.9 μM
Incubation Time: 24 h
Result: Induced apoptosis.
Increased the proportion of apoptotic cells.
Upregulated cleaved-PARP and Bax, and downregulated Bcl-2.

Cell Cycle Analysis[1]

Cell Line: 0.3, 0.6, 1.2 μM
Concentration: 0.3, 0.9 μM
Incubation Time: 24 h
Result: Induced G2/M phase arrest.
Upregulated Cyclin B1 and downregulated CDK1 and Cdc25c.
In Vivo

Tubulin-IN-55 (Compound 89) (10 mg/kg, i.p., once every two days, 4 weeks) demonstrates potent antitumor efficacy in orthotopic autologous transplantation BALB/c mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Tumor cells were implanted into 6-8-week-old BALB/c mice to establish the in vivo tumor model[1]
Dosage: 10 mg/kg
Administration: i.p., once every two days, 4 weeks
Result: Achieved tumor growth inhibition.
Suppressed breast cancer metastasis to the lung.
Reduced the Ki67 expression and PCNA expression in tumor tissues.
Disrupted the microtubule network in tumor tissues.
Showed no significant changes in body weight and major organ (heart, liver, spleen, lung, kidney) weights.
Molecular Weight

350.46

Formula

C21H26N4O
CAS No.
SMILES

O=C(N1CCN(CC1)C2=CC=CC=C2)C3=CC=CN=C3N4CCCCC4
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Tubulin-IN-55 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tubulin-IN-55353258-94-3Microtubule/TubulinPI3KAktApoptosisPhosphoinositide 3-kinasePKBProtein kinase BHeLaHCT1164T1A549H1299MDA-MB231tubulincancerInhibitorinhibitorinhibit

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