2. Apoptosis NF-κB Metabolic Enzyme/Protease Immunology/Inflammation Anti-infection Others
  3. Apoptosis Reactive Oxygen Species (ROS) Fungal Isotope-Labeled Compounds SOD Interleukin Related NF-κB Toll-like Receptor (TLR) Keap1-Nrf2
  4. Tomentosin

Tomentosin is an orally active natural sesquiterpenoid lactone. Tomentosin exhibits multiple activities such as anti-tumor, anti-fungal, anti-inflammatory, anti-oxidant and neuroprotective effects. Tomentosin can inhibit tumor cell proliferation, migration and invasion, and induce apoptosis. Tomentosin can be used in the research of tumors, inflammation and nervous system diseases.

For research use only. We do not sell to patients.

Tomentosin Chemical Structure

Tomentosin Chemical Structure

CAS No. : 33649-15-9

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Based on 1 publication(s) in Google Scholar

Tomentosin Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Tomentosin

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

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TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Tomentosin is an orally active natural sesquiterpenoid lactone. Tomentosin exhibits multiple activities such as anti-tumor, anti-fungal, anti-inflammatory, anti-oxidant and neuroprotective effects. Tomentosin can inhibit tumor cell proliferation, migration and invasion, and induce apoptosis. Tomentosin can be used in the research of tumors, inflammation and nervous system diseases[1][2][3][4].

IC50 & Target[2]

IL-6

 

IL-1β

 

TLR2

 

TLR4

 

Cellular Effect
Cell Line Type Value Description References
HT-29 IC50
56.5 μM
Compound: 9
Cytostatic activity against human HT-29 cells incubated for 72 hrs by alamar blue reagent based resazurin metabolism assay
Cytostatic activity against human HT-29 cells incubated for 72 hrs by alamar blue reagent based resazurin metabolism assay
[PMID: 26218649]
MCF7 IC50
> 50 μg/mL
Compound: 17
Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay
Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay
[PMID: 21109433]
MCF7 IC50
26.6 μM
Compound: 9
Cytostatic activity against human MCF7 cells incubated for 6 days by alamar blue reagent based resazurin metabolism assay
Cytostatic activity against human MCF7 cells incubated for 6 days by alamar blue reagent based resazurin metabolism assay
[PMID: 26218649]
RAW264.7 IC50
15.7 μM
Compound: 9
Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced thromboxane B2 production incubated for 48 hrs by ELISA method
Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced thromboxane B2 production incubated for 48 hrs by ELISA method
[PMID: 26218649]
RAW264.7 IC50
2 μM
Compound: 11
Inhibition of LPS-induced NO production in mouse RAW264.7 cells incubated for 24 hrs by Griess reagent based assay
Inhibition of LPS-induced NO production in mouse RAW264.7 cells incubated for 24 hrs by Griess reagent based assay
[PMID: 27276091]
RAW264.7 IC50
7.14 μM
Compound: 32
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins before LPS challenge measured 24 hrs after LPS challenge by Griess reaction method
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins before LPS challenge measured 24 hrs after LPS challenge by Griess reaction method
[PMID: 21924800]
RAW264.7 IC50
7.3 μM
Compound: 9
Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced prostaglandin E2 production incubated for 48 hrs by ELISA method
Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced prostaglandin E2 production incubated for 48 hrs by ELISA method
[PMID: 26218649]
RAW264.7 IC50
9.15 μM
Compound: 12
Inhibition of LPS-induced NO production in mouse RAW264.7 cells measured after 24 hrs
Inhibition of LPS-induced NO production in mouse RAW264.7 cells measured after 24 hrs
[PMID: 20515062]
In Vitro

Tomentosin (0-100 µM; 24-72 h) inhibits the proliferation, migration and invasion, induces apoptosis, and increases ROS levels and decreases mitochondrial membrane potential in pancreatic cancer cells PANC-1 and MIA PaCa-2[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tomentosin Related Antibodies

Cell Viability Assay[1]

Cell Line: PANC-1 and MIA PaCa-2 cells
Concentration: 5, 10, 15, 20, 25, 30, 40, 50, 75 and 100 μM
Incubation Time: 24, 48 and 72 h
Result: Inhibited proliferation of pancreatic cancer cells in a dose- and time-dependent manner.
Had IC50 values of 147.62 μM, 31.11 μM and 22.87 μM for 24, 48 and 72 h in PANC-1 cells.
Had IC50 values of 98.08 μM, 33.93 μM and 22.14 μM for 24, 48 and 72 h in MIA PaCa-2 cells.

Cell Viability Assay[1]

Cell Line: PANC-1 cells
Concentration: 31.11 μM
Incubation Time: 48 h
Result: Increased the levels of cleaved Caspase-3, and -9.
In Vivo

Tomentosin (25-50 mg/kg; 15 days) exerts neuroprotective effects in a Parkinson's disease mouse model[2].
Tomentosin (12.5-50 mg/kg; oral gavage; 10 days) improves cardiac dysfunction in a mouse model[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57/BL6 mice (23-35 g, 9-12 weeks old) treated MPTP (HY-W114750)[2]
Dosage: 25 and 50 mg/kg
Administration: 15 days
Result: Significantly prevented weight loss.
Improved motor function (evidenced by better performance in rota-rod test, grasping test, and pole-climbing test).
Reduced levels of proinflammatory cytokines (TNF-α, IL-1β, IL-6), ROS, and MPO, enhanced SOD activity.
Inhibited the expression of TLR-4, TLR-2, and NF-κB in brain tissues, and prevented glial cell damage and neuronal loss as shown by histopathological analysis.
Animal Model: Male Balb/c mice (28-33 g, 8-10 weeks old) with LPS (HY-D1056)[3]
Dosage: 12.5, 25 and 50 mg/kg
Administration: Oral gavage (i.g.); 10 days
Result: Mitigated cardiac injury and histopathological damage.
Significantly reduced Troponin and CK-MB levels.
Alleviated oxidative stress, and suppressed inflammatory responses.
Inhibited NF-κB activation while enhancing Nrf-2 expression.
Molecular Weight

248.32

Formula

C15H20O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C=C1[C@](CC=C2CCC(C)=O)([H])[C@@](OC1=O)([H])C[C@@H]2C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (201.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.0271 mL 20.1353 mL 40.2706 mL
5 mM 0.8054 mL 4.0271 mL 8.0541 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (5.03 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.25 mg/mL (5.03 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.0271 mL 20.1353 mL 40.2706 mL 100.6765 mL
5 mM 0.8054 mL 4.0271 mL 8.0541 mL 20.1353 mL
10 mM 0.4027 mL 2.0135 mL 4.0271 mL 10.0677 mL
15 mM 0.2685 mL 1.3424 mL 2.6847 mL 6.7118 mL
20 mM 0.2014 mL 1.0068 mL 2.0135 mL 5.0338 mL
25 mM 0.1611 mL 0.8054 mL 1.6108 mL 4.0271 mL
30 mM 0.1342 mL 0.6712 mL 1.3424 mL 3.3559 mL
40 mM 0.1007 mL 0.5034 mL 1.0068 mL 2.5169 mL
50 mM 0.0805 mL 0.4027 mL 0.8054 mL 2.0135 mL
60 mM 0.0671 mL 0.3356 mL 0.6712 mL 1.6779 mL
80 mM 0.0503 mL 0.2517 mL 0.5034 mL 1.2585 mL
100 mM 0.0403 mL 0.2014 mL 0.4027 mL 1.0068 mL
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Tomentosin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tomentosin33649-15-9ApoptosisReactive Oxygen Species (ROS)FungalIsotope-Labeled CompoundsSODInterleukin RelatedNF-κBToll-like Receptor (TLR)Keap1-Nrf2Superoxide DismutaseILNuclear factor-κBNuclear factor-kappaBParkinson's disease modelLPSPANC-1MIA PaCa-2Inhibitorinhibitorinhibit

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