2. Metabolic Enzyme/Protease Anti-infection Autophagy Apoptosis
  3. Carbonic Anhydrase Parasite Autophagy Apoptosis
  4. Tioxolone

Tioxolone is an inhibitor for the carbonic anhydrase. Tioxolone exhibits anti-leishmanial, antitumor, and anti-inflammatory activities. Tioxolone can be used in research of acne and psoriasis.

For research use only. We do not sell to patients.

Tioxolone Chemical Structure

Tioxolone Chemical Structure

CAS No. : 4991-65-5

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10 mM * 1 mL in DMSO
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Tioxolone:

Tioxolone Related Antibodies

Top Publications Citing Use of Products

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CA CA I CA Ⅱ CA IX CA XII

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Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Tioxolone is an inhibitor for the carbonic anhydrase. Tioxolone exhibits anti-leishmanial, antitumor, and anti-inflammatory activities. Tioxolone can be used in research of acne and psoriasis[1][2][3].

In Vitro

Tioxolone exhibits anti-leishmanial activity against amastigote and promastigote with IC50s of 49.8 μg/mL and 56.1 μg/mL[2].
Tioxolone (12.5-200 μg/mL, 48 h) dose-dependently induces apoptosis of Leishmania promastigote[2].
Tioxolone (12.5-200 μg/mL, 48 h) increases the expression of IL-12 and metacaspase, decreases the expression of IL-10, exhibiting immunomodulatory effect[2].
Tioxolone (10 µM, 12h) promotes the expression of LC3-II, reduces the expression of p62 protein, and induces autophagy in cancer cell A375[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tioxolone Related Antibodies

Cell Viability Assay[2]

Cell Line: Leishmania promastigote
Concentration: 12.5-200 μg/mL
Incubation Time: 48 h
Result: Induced apoptosis.

Western Blot Analysis[2]

Cell Line: A375
Concentration: 10 µM
Incubation Time: 12 h
Result: Increased the expression of LC3-II.
Molecular Weight

168.17

Formula

C7H4O3S
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1OC2=CC(O)=CC=C2S1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (594.63 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 0.67 mg/mL (3.98 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.9463 mL 29.7313 mL 59.4626 mL
5 mM 1.1893 mL 5.9463 mL 11.8925 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (14.87 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (14.87 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 5.9463 mL 29.7313 mL 59.4626 mL 148.6564 mL
DMSO 5 mM 1.1893 mL 5.9463 mL 11.8925 mL 29.7313 mL
10 mM 0.5946 mL 2.9731 mL 5.9463 mL 14.8656 mL
15 mM 0.3964 mL 1.9821 mL 3.9642 mL 9.9104 mL
20 mM 0.2973 mL 1.4866 mL 2.9731 mL 7.4328 mL
25 mM 0.2379 mL 1.1893 mL 2.3785 mL 5.9463 mL
30 mM 0.1982 mL 0.9910 mL 1.9821 mL 4.9552 mL
40 mM 0.1487 mL 0.7433 mL 1.4866 mL 3.7164 mL
50 mM 0.1189 mL 0.5946 mL 1.1893 mL 2.9731 mL
60 mM 0.0991 mL 0.4955 mL 0.9910 mL 2.4776 mL
80 mM 0.0743 mL 0.3716 mL 0.7433 mL 1.8582 mL
100 mM 0.0595 mL 0.2973 mL 0.5946 mL 1.4866 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Tioxolone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tioxolone4991-65-5Carbonic AnhydraseParasiteAutophagyApoptosisCarbonate dehydrataseA375anti-leishmanialimmunomodulatoryInhibitorinhibitorinhibit

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