Temporin-SHa 

Temporin-Sha is an antibacterial peptide with extensive biological activity. Temporin-Sha exhibits broad-spectrum antibacterial activity (e.g., against L. ivanovii, MIC = 6.25 μM), and is effective against Gram-negative bacteria (such as Escherichia coli, MIC = 10 μM), including drug-resistant strains (such as Methicillin (HY-121544)-resistant Staphylococcus aureus). Temporin-Sha also has inhibitory effects on Candida albicans (MIC = 25 μM), Saccharomyces cerevisiae (MIC = 12 μM), the pre-flagellated and non-flagellated forms of Leishmania infantum (IC₅₀ = 5-20 μM), and Trypanosoma cruzi (IC₅₀ = 17 μM). Temporin-Sha exhibits antiviral activity against HSV-1 and has anti-cancer effects (cytotoxicity against breast cancer cells MCF-7 and lung cancer cells H460, etc.)^{[1][2][3][4][5]}.

Temporin-Sha (12.5 μM, 3 h) causes the bacterial membrane of Listeria ivanovii to rupture, and even after being grafted onto the gold surface, it can still kill over 75% of the bacteria^[1].
Temporin-Sha (2-1024 μg/mL, 0-24 h) has a significant antifungal effect on Fluconazole (HY-B0101)-sensitive (CaS) and Fluconazole-resistant (CaR) Candida albicans (MIC ≥ 256 μg/mL), and can inhibit the formation of CaS and CaR biofilms, it still has the ability to destroy the formed biofilms at high concentrations (1024 μg/mL)^[2].
Temporin-Sha (32-1024 μg/mL) shows no cytotoxicity against normal oral keratinocytes (NOK-si) (CC₅₀ = 3805 μg/mL) at all concentrations, and exhibits mild cytotoxicity against human gingival fibroblasts (FGH) (CC₅₀ = 492 μg/mL)^[2].
Temporin-Sha (1.25-10 μM, 25 h) significantly inhibits HSV-1 DNA replication by directly destroying the virus particles (without relying on immune regulation) in human primary keratinocytes^[3].
Temporin-Sha (2-50 μM, 0-180 min) alters efficiently the integrity of the bacterial (E. coli, S. pyogenes, K. pneumoniae, L. infantum, S. aureus, and P. aeruginosa) and parasite (T. cruzi) plasma membrane and induce apoptotic-like death in Leishmania infantum promastigotes^[4].
Temporin-Sha (0-60 days) display no propensity to promote bacterial resistance after 55 consecutive generations of culture in E. coli, unlike Ampicillin (HY-B0522)^[4].
Temporin-SHa is cytotoxic to multiple cancer cell lines with IC₅₀ values of 14.47 μM (MCF-7), 18.36 μM (HeLa) and 34.5 μM (NCI-H460) and induced cell death is mainly necrotic rather than apoptotic^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1380.74

C₆₇H₁₀₉N₁₅O₁₄S

1065010-73-2

Phe-Leu-Ser-Gly-Ile-Val-Gly-Met-Leu-Gly-Lys-Leu-Phe-NH2

FLSGIVGMLGKLF-NH2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Lombana A, et al. Temporin-SHa peptides grafted on gold surfaces display antibacterial activity. J Pept Sci. 2014 Jul;20(7):563-9.  [Content Brief]

  [2]. Dias LM, et al. Antibiofilm Activity and Biocompatibility of Temporin-SHa: A Promising Antimicrobial Peptide for Control of Fluconazole-Resistant Candida albicans. Microorganisms. 2024 Jan 4;12(1):99.  [Content Brief]

  [3]. Roy M, et al. Comparison of Anti-Viral Activity of Frog Skin Anti-Microbial Peptides Temporin-Sha and [K³]SHa to LL-37 and Temporin-Tb against Herpes Simplex Virus Type 1. Viruses. 2019 Jan 18;11(1):77.  [Content Brief]

  [4]. Raja Z, et al. Insight into the mechanism of action of temporin-SHa, a new broad-spectrum antiparasitic and antibacterial agent. PLoS One. 2017 Mar 20;12(3):e0174024.  [Content Brief]

  [5]. Shaheen F, et aI. Synthesis of breast cancer targeting conjugate of temporin-SHa analog and its effect on pro- and anti-apoptotic protein expression in MCF-7 cells. Peptides. 2018 Aug;106:68-82.  [Content Brief]