2. Cytoskeleton
  3. Integrin
  4. TC-I 15

TC-I 15 (TC-I-15) is a type of allosteric collagen-binding integrin α2β1 inhibitor, and it also inhibits α1β1 and α11β1. TC-I 15 inhibits platelet adhesion to collagen and thrombus deposition. TC-I 15 prevents the formation of a pre-metastatic microenvironment by inhibiting the uptake of cancer-associated fibroblast (CAF) extracellular vesicles (EVs) by lung fibroblasts, which reduces the metastasis of salivary gland adenocystic carcinoma (SACC) to the lungs in mouse models, .

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TC-I 15 Chemical Structure

TC-I 15 Chemical Structure

CAS No. : 916734-43-5

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

TC-I 15 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE TC-I 15

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View All Isoforms
αvβ1 αvβ3 αvβ5 αvβ6 αvβ8 α5β1 α1β1 α2β1 α4β1 α9β1 αIIbβ3 α4β7 αLβ2

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Description

TC-I 15 (TC-I-15) is a type of allosteric collagen-binding integrin α2β1 inhibitor, and it also inhibits α1β1 and α11β1. TC-I 15 inhibits platelet adhesion to collagen and thrombus deposition. TC-I 15 prevents the formation of a pre-metastatic microenvironment by inhibiting the uptake of cancer-associated fibroblast (CAF) extracellular vesicles (EVs) by lung fibroblasts, which reduces the metastasis of salivary gland adenocystic carcinoma (SACC) to the lungs in mouse models, [1][2].

IC50 & Target

α2β1

0.4 (α2β1 μM (IC50)

α2β1

26.8 (α2β1 μM (IC50)

α1β1

23.6 (α1β1 μM (IC50)

α1β1

24.4 (α1β1 μM (IC50)

α11β1

177 (α11β1 μM (IC50)

α11β1

3177 (α11β μM (IC50)

In Vitro

TC-I 15 (0-500 μM, 1 h) inhibits the adhesion of C2C12 cells expressing α2β1 in a dose-dependent manner, with IC50 values of 26.8 μM and 0.4 μM for GFOGER and GLOGEN, respectively[1].
TC-I 15 (0-500μM, 1 h) also inhibited α1β1, showing IC50 values of 23.6 μM for GFOGER and 24.4 μM for GLOGEN[1].
TC-I 15 (0-50μM, 1 h) inhibits the adhesion of HT1080 cells to GFOGER[1].
TC-I 15 (0-50μM, 1 h) inhibits the uptake of EVs by LFs cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

TC-I 15 Related Antibodies

Western Blot Analysis[2]

Cell Line: LFs
Concentration: 0, 0.5, 1, 2 μM
Incubation Time: 1 h
Result: Inhibited LFs uptake of EVs, inhibited LF activation induced by EVs, and inhibited the expression of p-Smad3 and POSTN.
In Vivo

TC I-15 (10 μg; intravenous injection; every other day; for three weeks) inhibits lung metastasis promoted by CAFs EVs in mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 J mice[2]
Dosage: 10 μg; every other day for 3 weeks; once every 2 weeks for 3 weeks
Administration: Intravenous injection (i.v.)
Result: Inhibited the formation of pre-metastatic microenvironment induced by EVs, inhibited SACC lung metastasis induced by EVs, and significantly increased integrin β1 of EVs.
Molecular Weight

520.62

Formula

C23H28N4O6S2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=[S](N(CSC(C)1C)[C@@H]1C(N[C@H](C(O)=O)CNC(NCC2=CC=CC=C2)=O)=O)(C3=CC=CC=C3)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (480.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9208 mL 9.6039 mL 19.2079 mL
5 mM 0.3842 mL 1.9208 mL 3.8416 mL
View the Complete Stock Solution Preparation Table

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.00 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (4.00 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  50% PEG300    50% PBS

    Solubility: 10 mg/mL (19.21 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
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The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9208 mL 9.6039 mL 19.2079 mL 48.0197 mL
5 mM 0.3842 mL 1.9208 mL 3.8416 mL 9.6039 mL
10 mM 0.1921 mL 0.9604 mL 1.9208 mL 4.8020 mL
15 mM 0.1281 mL 0.6403 mL 1.2805 mL 3.2013 mL
20 mM 0.0960 mL 0.4802 mL 0.9604 mL 2.4010 mL
25 mM 0.0768 mL 0.3842 mL 0.7683 mL 1.9208 mL
30 mM 0.0640 mL 0.3201 mL 0.6403 mL 1.6007 mL
40 mM 0.0480 mL 0.2401 mL 0.4802 mL 1.2005 mL
50 mM 0.0384 mL 0.1921 mL 0.3842 mL 0.9604 mL
60 mM 0.0320 mL 0.1601 mL 0.3201 mL 0.8003 mL
80 mM 0.0240 mL 0.1200 mL 0.2401 mL 0.6002 mL
100 mM 0.0192 mL 0.0960 mL 0.1921 mL 0.4802 mL
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TC-I 15 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TC-I 15916734-43-5Integrincollagen-binding integrinGFOGERGLOGENplatelet adhesionthrombus depositioncell adhesionInhibitorinhibitorinhibit

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