STAT3-IN-14

Name: STAT3-IN-14
Brand: MedChemExpress
SKU: HY-N10472
Rating: 4.5 (1 reviews)

Cat. No.: HY-N10472 Purity: 95.89%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

STAT3-IN-14 (Compound 1) is a STAT3 inhibitor and has STAT3 phosphorylation inhibitory activity. STAT3-IN-14 (Compound 1) can directly bind to the hinge region of STAT3.

For research use only. We do not sell to patients.

STAT3-IN-14 Chemical Structure

CAS No. : 123297-90-5

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

STAT3-IN-14 (Compound 1) is a STAT3 inhibitor and has STAT3 phosphorylation inhibitory activity. STAT3-IN-14 (Compound 1) can directly bind to the hinge region of STAT3^[1].

IC₅₀ & Target

IC50: 0.42 μM (MDA-MB-231); 0.50 μM (MCF7); 0.13 μM (A549) ^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.13 μM Compound: 1	Cytotoxicity against human A549 cells assessed as reduction in cell proliferation measured after 72 hrs by WST8 assay Cytotoxicity against human A549 cells assessed as reduction in cell proliferation measured after 72 hrs by WST8 assay	[PMID: 32044231]
A549	EC₅₀	0.78 μM Compound: 1	Antiproliferative activity against human A549 cells after 72 hrs by trypan blue exclusion assay Antiproliferative activity against human A549 cells after 72 hrs by trypan blue exclusion assay	[PMID: 19674905]
A549	EC₅₀	0.96 μM Compound: 1	Cytotoxicity against human A549 cells assessed as cell viability by tryptan blue staining method Cytotoxicity against human A549 cells assessed as cell viability by tryptan blue staining method	[PMID: 17950604]
Fibroblast	EC₅₀	11.1 μM Compound: 1	Antiproliferative activity against human skin fibroblast after 72 hrs by trypan blue exclusion assay Antiproliferative activity against human skin fibroblast after 72 hrs by trypan blue exclusion assay	[PMID: 19674905]
Hepatocyte	EC₅₀	11.1 μM Compound: 1	Antiproliferative activity against human hepatocytes after 72 hrs by trypan blue exclusion assay Antiproliferative activity against human hepatocytes after 72 hrs by trypan blue exclusion assay	[PMID: 19674905]
MCF7	IC₅₀	0.5 μM Compound: 1	Cytotoxicity against human MCF7 cells assessed as reduction in cell proliferation measured after 72 hrs by WST8 assay Cytotoxicity against human MCF7 cells assessed as reduction in cell proliferation measured after 72 hrs by WST8 assay	[PMID: 32044231]
MCF7	EC₅₀	0.51 μM Compound: 1	Antiproliferative activity against human MCF7 cells after 72 hrs by trypan blue exclusion assay Antiproliferative activity against human MCF7 cells after 72 hrs by trypan blue exclusion assay	[PMID: 19674905]
MCF7	IC₅₀	2 μM Compound: 1	Inhibition of IL-6 induced STAT3 phosphorylation at Tyr705 residue in human MCF7 cells preincubated for 1 hr followed by IL-6 stimulation and measured after 1 hr by Western blot analysis Inhibition of IL-6 induced STAT3 phosphorylation at Tyr705 residue in human MCF7 cells preincubated for 1 hr followed by IL-6 stimulation and measured after 1 hr by Western blot analysis	[PMID: 32044231]
MCF7	EC₅₀	3.5 μM Compound: 1	Cytotoxicity against human MCF7 cells assessed as cell viability by tryptan blue staining method Cytotoxicity against human MCF7 cells assessed as cell viability by tryptan blue staining method	[PMID: 17950604]
MDA-MB-231	IC₅₀	0.41 μM Compound: 1	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell proliferation measured after 72 hrs by WST8 assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell proliferation measured after 72 hrs by WST8 assay	[PMID: 32044231]
PC-3	EC₅₀	0.14 μM Compound: 1	Antiproliferative activity against human PC3 cells after 72 hrs by trypan blue exclusion assay Antiproliferative activity against human PC3 cells after 72 hrs by trypan blue exclusion assay	[PMID: 19674905]
PC-3	EC₅₀	0.14 μM Compound: 1	Cytotoxicity against human PC3 cells assessed as cell viability by tryptan blue staining method Cytotoxicity against human PC3 cells assessed as cell viability by tryptan blue staining method	[PMID: 17950604]
Raji	IC₅₀	33.2 μM Compound: 1	Inhibition of 12-O-tetradecanoylphorbol-13-acetate-induced EBV-early antigen activation in human Raji cells after 48 hrs by immunofluorescence technique Inhibition of 12-O-tetradecanoylphorbol-13-acetate-induced EBV-early antigen activation in human Raji cells after 48 hrs by immunofluorescence technique	[PMID: 19674905]

In Vitro

STAT3-IN-14 (Compound 1) shows inhibitory activity against phosphor-STAT3.
STAT3-IN-14 (Compound 1) reduces the phosphorylation inhibitory activity against MDA-MB-231 cells (JAK/STAT pathway constitutively active) in does dependent manner, but no inhibitory activities against JAK kinases.
STAT3-IN-14 (Compound 1) has growth inhibitory activity with with IC₅₀ values of 0.42 μM, 0.50 μM and 0.13 μM in MDA-MB-231, MCF7 and A549cells, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	MCF7 cells; MDA-MB-231 cells
Concentration:	1 or 10 μM; 0.5, 1, 2, 4 μM
Incubation Time:	1 h; 2 h
Result:	Completely inhibited STAT3 phosphorylation at 10 μM, and partial inhibition at 1 μM. Reduced the phosphorylation of STAT3 in does dependent manner.

Cell Proliferation Assay^[1]

Cell Line:	MDA-MB-231, MCF7 and A549 cells
Concentration:	0-30 μM
Incubation Time:	72 h
Result:	Showed moderate to strong growth inhibitory activity among the three cancer cell lines.

Molecular Weight

258.23

Formula

C₁₄H₁₀O₅

CAS No.

123297-90-5

Appearance

Solid

Color

Light brown to yellow

SMILES

O=C(C1=C2OC([C@@H](O)C)=C1)C3=C(C2=O)C=CC=C3O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 120 mg/mL (464.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8725 mL	19.3626 mL	38.7252 mL
5 mM	0.7745 mL	3.8725 mL	7.7450 mL
10 mM	0.3873 mL	1.9363 mL	3.8725 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 95.89%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (251 KB) HNMR (140 KB) RP-HPLC (261 KB) LCMS (104 KB)

Handling Instructions (2659 KB)

References

[1]. Teruyuki Tahara, et al. STAT3 inhibitory activity of naphthoquinones isolated from Tabebuia avellanedae. Bioorg Med Chem. 2020 Mar 15;28(6):115347. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.8725 mL	19.3626 mL	38.7252 mL	96.8129 mL
	5 mM	0.7745 mL	3.8725 mL	7.7450 mL	19.3626 mL
	10 mM	0.3873 mL	1.9363 mL	3.8725 mL	9.6813 mL
	15 mM	0.2582 mL	1.2908 mL	2.5817 mL	6.4542 mL
	20 mM	0.1936 mL	0.9681 mL	1.9363 mL	4.8406 mL
	25 mM	0.1549 mL	0.7745 mL	1.5490 mL	3.8725 mL
	30 mM	0.1291 mL	0.6454 mL	1.2908 mL	3.2271 mL
	40 mM	0.0968 mL	0.4841 mL	0.9681 mL	2.4203 mL
	50 mM	0.0775 mL	0.3873 mL	0.7745 mL	1.9363 mL
	60 mM	0.0645 mL	0.3227 mL	0.6454 mL	1.6135 mL
	80 mM	0.0484 mL	0.2420 mL	0.4841 mL	1.2102 mL
	100 mM	0.0387 mL	0.1936 mL	0.3873 mL	0.9681 mL

STAT3-IN-14 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

STAT3-IN-14123297-90-5STATSTAT3 inhibitorJAK/STAT pathwayMDA-MB-231MCF7 A549Inhibitorinhibitorinhibit

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STAT3-IN-14

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STAT3-IN-14 Related Antibodies

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References

Customer Review

STAT3-IN-14 Related Antibodies

Molarity Calculator

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Complete Stock Solution Preparation Table

STAT3-IN-14 Related Classifications

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