2. Cell Cycle/DNA Damage Metabolic Enzyme/Protease
  3. HSP
  4. SEW84

SEW84 is an inhibitor of the Aha1stimulated Hsp90 (ASH) ATPase activity. SEW84 inhibit ASH activity with an IC50 value of 0.3 μM. SEW84 can be used for the research of proteostatic disease.

SEW84 is an inhibitor of Aha1-stimulated Hsp90 (ASH) ATPase activity. SEW84 inhibits Aha1-stimulated Hsp90 (ASH) activity with an IC50 value of 0.3 μM. SEW84 can be used in the study of protein deposition diseases.

For research use only. We do not sell to patients.

SEW84

SEW84 Chemical Structure

CAS No. : 259089-67-3

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Based on 1 publication(s) in Google Scholar

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Description

SEW84 is an inhibitor of Aha1-stimulated Hsp90 (ASH) ATPase activity. SEW84 inhibits Aha1-stimulated Hsp90 (ASH) activity with an IC50 value of 0.3 μM. SEW84 can be used in the study of protein deposition diseases[1].

IC50 & Target

IC50: 0.3 μM (ASH); 1.3 μM (GR-dependent luciferase expression); 0.7 μM (AR-dependent luciferase expression)[1]
In Vitro

SEW84 (5 μM) shows complete inhibition of ASH activity with an IC50 value of 0.3 μM[1].
SEW84 inhibited the GR- and AR-dependent luciferase expression with IC50 values of 1.3 and 0.7 μM, respectively[1].
SEW84 (0-20 μM) inhibits the Hsp90-Dependent folding of firefly luciferase[1].
SEW84 (0, 3, 10, 30 μM; 24 h) specifically clears phosphorylated tau[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SEW84 Related Antibodies

Western Blot Analysis[1]

Cell Line: tau-0N4R-HeLa cells
Concentration: 0, 3, 10, 30 μM
Incubation Time: 24 h
Result: Reduced the expression of phospho-Tau.

Immunofluorescence[1]

Cell Line: RFP-tau-0N4R HEK cells
Concentration: 1, 10 μM
Incubation Time: 24 h
Result: Completely abrogated the aggregation of tau.

Cell Proliferation Assay[1]

Cell Line: LNCaP and 22Rv1 cells
Concentration: 0-100 μM
Incubation Time: 24 h
Result: Observed a synergistic decrease in LNcaP cell proliferation when combined 1 μM GA with 10 μM SEw84.
Molecular Weight

422.40

Formula

C19H14F4N4OS
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=CN1C2=CC=C(C=C2)F)NNC(NC3=CC=CC(C(F)(F)F)=C3)=S
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (295.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3674 mL 11.8371 mL 23.6742 mL
5 mM 0.4735 mL 2.3674 mL 4.7348 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.73%

SDS (251 KB)

COA (251 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3674 mL 11.8371 mL 23.6742 mL 59.1856 mL
5 mM 0.4735 mL 2.3674 mL 4.7348 mL 11.8371 mL
10 mM 0.2367 mL 1.1837 mL 2.3674 mL 5.9186 mL
15 mM 0.1578 mL 0.7891 mL 1.5783 mL 3.9457 mL
20 mM 0.1184 mL 0.5919 mL 1.1837 mL 2.9593 mL
25 mM 0.0947 mL 0.4735 mL 0.9470 mL 2.3674 mL
30 mM 0.0789 mL 0.3946 mL 0.7891 mL 1.9729 mL
40 mM 0.0592 mL 0.2959 mL 0.5919 mL 1.4796 mL
50 mM 0.0473 mL 0.2367 mL 0.4735 mL 1.1837 mL
60 mM 0.0395 mL 0.1973 mL 0.3946 mL 0.9864 mL
80 mM 0.0296 mL 0.1480 mL 0.2959 mL 0.7398 mL
100 mM 0.0237 mL 0.1184 mL 0.2367 mL 0.5919 mL
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SEW84 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SEW84259089-67-3SEW04784SEW 84SEW-84SEW 04784SEW-04784HSPHeat shock proteinsAha1stimulated Hsp90 (ASH)ATPaseproteostatic diseaseInhibitorinhibitorinhibit

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