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Protein assay

" in MedChemExpress (MCE) Product Catalog:

By Targets:	Biochemical Assay Reagents (17) AAK1 (2) ADC Linker (1) Adenylate Cyclase (1) Adrenergic Receptor (1) Amyloid-β (1) Apoptosis (11) Aryl Hydrocarbon Receptor (2) ATP Synthase (1) Aurora Kinase (1) Autophagy (8) Bacterial (1) Bcr-Abl (1) BCRP (2) Beta-lactamase (5) Beta-secretase (1) c-Kit (1) c-Myc (1) CaMK (1) CETP (2) Cholinesterase (ChE) (1) DAPK (1) Dopamine Receptor (1) EHD Protein (1) Endogenous Metabolite (3) Epigenetic Reader Domain (2) FLAP (3) Fluorescent Dye (11) Fungal (1) HDAC (6) Histamine Receptor (1) Histone Methyltransferase (2) HIV (1) IAP (1) Integrin (2) Isocitrate Dehydrogenase (IDH) (1) Keap1-Nrf2 (2) Lipocalin Family (1) Liposome (2) Lipoxygenase (1) mAChR (1) MAPKAPK2 (MK2) (1) MDM-2/p53 (2) MMP (1) Orphan Receptor (1) Phosphatase (1) PKC (3) PROTAC Linkers (1) Protease Activated Receptor (PAR) (1) Raf (1) Ras (1) Reference Standards (1) RIP kinase (3) RSV (1) Serotonin Transporter (1) sFRP-1 (2) TMV (1) Trk Receptor (1) VEGFR (4) Wnt (2)
By Research Areas:	Cancer (29) Cardiovascular Disease (5) Endocrinology (1) Infection (4) Inflammation/Immunology (10) Metabolic Disease (1) Neurological Disease (12)
Click Chemistry:	Azide (1) Alkynes (1)
Oligonucleotides:	mRNA (1)

103

Inhibitors & Agonists

Fluorescent Dye

Biochemical Assay Reagents

Peptides

MCE Kits

Natural
Products

Click Chemistry

Oligonucleotides

Targets Recommended: Biochemical Assay Reagents

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-15941

5(6)-FITC 2 Publications Verification Fluorescein 5(6)-isothiocyanate; Fluorescein isothiocyanate 5- and 6- isomers	Fluorescent Dye	Others
5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is an amine-reactive derivative of a fluorescent dye, characterized by high absorbance and excellent fluorescence quantum yield. The isothiocyanate group of FITC can react with various functional groups on proteins, including amines, thiols, imidazoles, tyrosines and carbonyls, enabling the labeling of proteins such as antibodies and lectins. 5(6)-FITC has a wide range of applications, including flow cytometry, immunofluorescence, protease assays and conjugation. The maximum excitation/emission wavelengths are 492/518 nm .

HY-18717

Mutant IDH1-IN-2 2 Publications Verification	Isocitrate Dehydrogenase (IDH)	Cancer
Mutant IDH1-IN-2 is a inhibitor of mutant Isocitrate dehydrogenase (IDH) proteins, with IC50 of in LS-MS biochemical assay, IC50 of 16.6 nM in Fluorescence biochemical assay.

HY-Y0537C

Potassium chloride, ≥99.99% trace metals basis Potassium chloride, suitable for the extraction and solubilization of Proteins	Biochemical Assay Reagents	Others
Potassium chloride, ≥99.99% trace metals basis is a biochemical assay reagent suitable for protein extraction and solubilization.

HY-P5426

Abl protein tyrosine kinase substrate	Bcr-Abl	Others
Abl protein tyrosine kinase substrate is a biological active peptide. (Abltide is a peptide substrate for Abl Kinase (Abl protein tyrosine kinase), a partner in the gag-Abl fusion protein of the Abelson murine leukemia virus. Used in Western blot and kinase assay.)

HY-149245

Keap1-Nrf2-IN-15	Keap1-Nrf2	Inflammation/Immunology
Keap1-Nrf2-IN-15 (Compound 24a) is a potent *Keap1-Nrf2 protein-protein* interaction inhibitor with IC₅₀*s of 77 nM and 2.5 nM in FP assay* and TR-FRET assay, respectively .

HY-157919

Biotin-PEAC5-maleimide hydrochloride N-6-(Biotinylamino)hexanoyl-N'-[2-(N-maleimido)ethyl]piperazine, hydrochloride	Biochemical Assay Reagents	Others
Biotin-PEAC5-maleimide hydrochloride is a biochemical assay reagent, and can be used in biotin-PEAC5-maleimide (BPM)-labeling assay to assess modification of protein thiols by electrophiles .

HY-129037

BCRP-IN-1	BCRP	Cancer
BCRP-IN-1 (compound 48) is a potent inhibitor of breast cancer resistance protein (BCRP), with IC₅₀s of 2.92 μM and 2.46 μM in Hoechst 33342 assay and Pheophorbide A assay, respectively .

HY-W741557

Griseofulvic Acid (±)-Griseofulvic acid	Fungal	Infection
Griseofulvic acid is a metabolite of the antifungal Griseofulvin (HY-17583). It induces protein aggregation and tubulin polymerization in cell-free assays.

HY-19614

BMS-795311	CETP	Cardiovascular Disease
BMS-795311 is a potent and orally bioavailable inhibitor of *cholesteryl ester transfer protein* (CETP), with IC₅₀*s of 4 nM in an enzyme-based scintillation proximity assay* (SPA) and 0.22 μM in a human whole plasma assay (hWPA), respectively .

HY-W558351

Z-Lys-OBzl benzenesulfonate	Biochemical Assay Reagents	Others
Z-Lys-OBzl benzenesulfonate is a biochemical assay reagent, which can be used as reference compound in research about succinylated protein .

HY-P0225

Autocamtide 2 Autocamtide II	CaMK Autophagy	Neurological Disease
Autocamtide 2 is a highly selective peptide substrate of calcium/calmodulin-dependent protein kinase II (CaMKII). It can be used in the CaMKII activity assay.

HY-16586

PFI-1 2 Publications Verification	Epigenetic Reader Domain Autophagy Apoptosis	Cancer
PFI-1 is a selective BET (bromodomain-containing protein) inhibitor for BRD4 with IC₅₀ of 0.22 μM in a cell-free assay.

HY-151884

FUBP1-IN-2	c-Myc	Cancer
FUBP1-IN-2 (compound 9) is a potent FUBP1 (far upstream binding protein 1) inhibitor. FUBP1-IN-2 inhibits the KH4 FUBP1-FUSE interaction in a gel shift assay. FUBP1-IN-2 binds to FUBP1 in a ChIP assay. FUBP1-IN-2 reduces both c-Myc mRNA and protein expression, increases p21 mRNA and protein expression, and depletes intracellular polyamines .

HY-13343

ZM 336372 2 Publications Verification	Raf Apoptosis	Cancer
ZM 336372 is a potent inhibitor of the protein kinase c-Raf. The IC₅₀ value is 0.07 μM in the standard assay, which contains 0.1 mM ATP.

HY-D0018

DCIP sodium Dichlorophenylindophenol sodium; DCPIP sodium; Indochlorophenol sodium	Biochemical Assay Reagents	Others
DCIP sodium is a blue dye commonly used in various biochemical and biotechnological applications as an indicator of redox reactions. DCIP sodium has unique chemical properties that change color according to the oxidation state of the substance being tested. It is commonly used in enzyme assays, such as measuring the activity of succinate dehydrogenase, or in protein quantification methods, such as the Lowry assay.

HY-172452

RSV L-protein-6	RSV	Infection
RSV L-protein-6 (Compound 19a) is a respiratory syncytial virus polymerase inhibitor, with an IC₅₀ of 13 nM in SPA primer extensions enzymatic assay. RSV L-protein-6 can inhibit RSV replication in Hela cells .

HY-141572

1,2-Dioleoyl-sn-glycerol sn-1,2-Dioleoylglycerol	Biochemical Assay Reagents	Others
ALN29882 is a glycerolipid located on the plasma membrane. It consists of two fatty acid chains covalently linked to a single glycerol molecule by means of an ester bond. 18:1 DG has been used as a source of diacylglycerol in the diacylglycerol O-acyltransferase 1 (DGAT1) assay. It is also used as a substrate in the DGAT-1 enzyme assay to evaluate compounds as potential inhibitors of DGAT-1. Suitable for lipoprotein overlay screening assays with the recombinant protein His-AtROP6.

HY-15917A

L-Dithiothreitol 1 Publications Verification	Biochemical Assay Reagents	Others
L-Dithiothreitol (DTT) is a reducing agent commonly used in various biochemical applications to break disulfide bonds in proteins, thereby denaturing proteins or preventing the formation of unwanted aggregates. DTT has a unique chemical property that cleaves the sulfur-sulfur bond in the disulfide bond to form a sulfhydryl group. This makes it a useful tool for protein purification, enzyme assays, and protein structure studies.

HY-147366

RBP4 ligand-1	Lipocalin Family	Metabolic Disease
RBP4 ligand-1 is a non-retinoid ligands for (RBP4) retinol-binding protein 4. The hRBP4_SPA IC₅₀ and RBP4-TTR FRET IC₅₀ values are 0.23±0.11 and 0.13±0.12 μM in the radioligand binding assay and FRET assay, respectively .

HY-W800659

Boc-Gly-Gly-NHS ester	ADC Linker	Others
Boc-Gly-Gly-NHS ester can be used to selectively attach small molecules to specific amino acid residues on proteins, creating conjugates that can be used for a variety of applications in drug discovery and diagnostic assays.

HY-19753

KS176 2 Publications Verification	BCRP	Cancer
KS176 is a potent and selective inhibitor of the breast cancer resistance protein (BCRP) multidrug transporter (IC50 values are 0.59 and 1.39 μM in Pheo A and Hoechst 33342 assays respectively).

HY-112157

PF-06751979 1 Publications Verification	Beta-secretase	Neurological Disease
PF-06751979 is a potent, brain penetrant, β-site amyloid precursor protein cleaving enzyme 1 (BACE1) inhibitor with an IC₅₀ of 7.3 nM in BACE1 binding assay.

HY-W437745

Desthiobiotin-NHS ester	Biochemical Assay Reagents	Others
Desthiobiotin-NHS ester is a biotinylated biochemical assay reagent that reacts with amine groups (such as lysine residues) of proteins or other biomolecules to form stable amide bonds. Desthiobiotin-NHS ester can be used as probes for metalloproteins .

HY-171493

mCherry mRNA(N1-Me-Pseudo UTP)	Biochemical Assay Reagents	Others
mCherry mRNA(N1-Me-Pseudo UTP) is a reporter mRNA encoding a red fluorescent protein, suitable for gene expression analysis, cell viability assays, and in vivo imaging. mCherry, derived from mushroom coral, is a widely used red fluorescent protein tracer in biotechnology applications.

HY-P1821A

Myelin Basic Protein TFA 2 Publications Verification MHP4-14 TFA	PKC	Neurological Disease
Myelin Basic Protein (MHP4-14) TFA, a synthetic peptide comprising residues 4-14 of myelin basic protein, is a very selective PKC substrate (K_m=7 μM). Myelin Basic Protein TFA is not phosphorylated by cyclic AMP-dependent protein kinase, casein kinases I and II, Ca ²⁺/calmodulin-dependent protein kinase II, or phosphorylase kinase, and can be routinely used for the assay of protein kinase C with low background in the crude tissue extracts .

HY-P1821

Myelin Basic Protein 2 Publications Verification MHP4-14	PKC	Neurological Disease
Myelin Basic Protein (MHP4-14), a synthetic peptide comprising residues 4-14 of myelin basic protein, is a very selective PKC substrate (K_m=7 μM). Myelin Basic Protein is not phosphorylated by cyclic AMP-dependent protein kinase, casein kinases I and II, Ca ²⁺/calmodulin-dependent protein kinase II, or phosphorylase kinase, and can be routinely used for the assay of protein kinase C with low background in the crude tissue extracts .

HY-D0018R

DCIP (sodium) (Standard)	Biochemical Assay Reagents	Others
DCIP (sodium) (Standard) is the analytical standard of DCIP (sodium). This product is intended for research and analytical applications. DCIP sodium is a blue dye commonly used in various biochemical and biotechnological applications as an indicator of redox reactions. DCIP sodium has unique chemical properties that change color according to the oxidation state of the substance being tested. It is commonly used in enzyme assays, such as measuring the activity of succinate dehydrogenase, or in protein quantification methods, such as the Lowry assay.

HY-N2853R

D-α-Tocopherylquinone (Standard)	Others	Cancer
DCIP (sodium) (Standard) is the analytical standard of DCIP (sodium). This product is intended for research and analytical applications. DCIP sodium is a blue dye commonly used in various biochemical and biotechnological applications as an indicator of redox reactions. DCIP sodium has unique chemical properties that change color according to the oxidation state of the substance being tested. It is commonly used in enzyme assays, such as measuring the activity of succinate dehydrogenase, or in protein quantification methods, such as the Lowry assay.

HY-P1376A

G-Protein antagonist peptide TFA	mAChR Adrenergic Receptor	Endocrinology
G-Protein antagonist peptide TFA is a truncated substance P-related peptide, competes with receptor for G protein binding. G-Protein antagonist peptide TFA inhibits the activation of G_i or G_o by M2 muscarinic cholinergic receptor (M2 mAChR) or of G_s by beta-adrenergic receptor in the reconstituted phospholipid vesicles, assayed by receptor-promoted GTP hydrolysis .

HY-W806047

BRD4 Inhibitor-37	Epigenetic Reader Domain	Cancer
BRD4 Inhibitor-37 is a compound with anticancer activity that has inhibitory activity against BRD4. BRD4 Inhibitor-37 has an IC50 of approximately 0.05-0.1 μM in binding assays and shows a GI50 of 0.1-0.3 μM in cell-based assays. The effect of BRD4 Inhibitor-37 on c-Myc, a downstream protein of BRD4, has been validated, demonstrating its ability to intervene in this signaling pathway. BRD4 Inhibitor-37 exhibits selectivity among five different bromodomain proteins, enhancing its potential as a BET protein inhibitor .

HY-153232

eGFP circRNA-LNP	Fluorescent Dye Liposome	Others
eGFP circRNA-LNP is a lipid nanoparticle (LNP) containing eGFP circRNA, suitable for assays of RNA delivery, translation efficiency, cell viability, etc. eGFP circRNA carries Enhanced Green Fluorescent Protein (Enhanced Green Fluorescent Protein) eGFP, which will express green fluorescent protein after entering the cell. eGFP is commonly used as a reporter gene detectable by fluorescence microscopy or flow cytometry .

HY-P3940

Ac-MBP (4-14) Peptide	PKC	Others
Ac-MBP (4-14) Peptide is an acetylated MBP (4-14) peptide. MBP (4-14) Peptide is a very selective (protein kinase C) PKC substrate. Ac-MBP (4-14) Peptide can be used for PKC assay in extracts without prior purification to eliminate interfering protein kinases or phosphatases .

HY-D0014

Brilliant blue G-250 3 Publications Verification	Biochemical Assay Reagents	Others
Brilliant Blue G-250 is a dye commonly used for the visualization of proteins separated by SDS-PAGE, offering a simple staining procedure and high quantitation. In the Bradford protein assay, protein concentrations are determined by the absorbance at 595 nm due to the binding of Brilliant Blue G-250 to proteins. Brilliant Blue G-250 is a safe highly selective P2×7R antagonist with promising consequent inactivation of NLRP3 inflammasome .

HY-120760

L-691831	Lipoxygenase	Others
L-691831 is a ligand for 5-lipoxygenase activating protein binding assays that has utility as a tool for studying 5-lipoxygenase activating protein (FLAP) related activities. L-691831 can be used to measure the affinity of FLAP on the leukocyte membrane for leukotriene synthesis inhibitors, with binding being correlated with inhibition of leukotriene synthesis.

HY-10858A

WAY 316606 hydrochloride	sFRP-1 Wnt	Cancer
WAY 316606 hydrochloride is an inhibitor of the secreted protein sFRP-1, an endogenous antagonist of the secreted glycoprotein Wnt. The affinity of WAY-316606 for sFRP-1 is determined using the FP binding assay with IC₅₀ of 0.5 μM .

HY-10858

WAY 316606 4 Publications Verification	sFRP-1 Wnt	Cancer
WAY 316606 is an inhibitor of the secreted protein sFRP-1, an endogenous antagonist of the secreted glycoprotein Wnt. The affinity of WAY-316606 for sFRP-1 is determined using the FP binding assay with IC₅₀ of 0.5 μM .

HY-160685

GW809897X	VEGFR	Others
GW809897X is a dual inhibitor of Fluc and VEGFR with IC₅₀ of 0.58 μM and 65 nM, respectively. GW809897X is a protein kinase inhibitor across ATP-dependent and -independent luciferases with potential impact on Fluc reporter assays .

HY-160684

GW701427A	VEGFR	Others
GW701427A is a dual inhibitor of Fluc and VEGFR2 with IC₅₀ of 0.12 μM and 603 nM, respectively. GW701427A acts as a protein kinase inhibitor across ATP-dependent and -independent luciferases with potential implications for Fluc reporter assays .

HY-139504

Gamcemetinib CC-99677	MAPKAPK2 (MK2)	Inflammation/Immunology
Gamcemetinib (CC-99677) is a potent, covalent, and irreversible inhibitor of the mitogen-activated protein (MAP) kinase-activated protein kinase-2 (MK2) pathway in both biochemical (IC₅₀=156.3 nM) and cell based assays (EC₅₀=89 nM). Gamcemetinib is extracted from patent WO2020236636, compound 1 .

HY-153080

Firefly Luciferase mRNA(N1-Me-Pseudo UTP)	Fluorescent Dye	Others
Firefly luciferase mRNA will express a luciferase protein, originally isolated from the Photinus pyralis (Firefly). Firefly Luciferase is a frequently used bioluminescent reporter for gene regulation and function study. It is applicable in assays for gene expression, cell viability and in vivo imaging etc.

HY-158415

ATPase-IN-4	ATP Synthase EHD Protein	Cancer
ATPase-IN-4 (Compound MS1) inhibits the ATPase activity of EHD4 (IC₅₀: 0.92 μM). ATPase-IN-4 also inhibits EHD2 enzymatic activity

HY-160655

SERT-IN-3	Serotonin Transporter	Neurological Disease
SERT-IN-3 (compound 12) is an inhibitor of serotonin transporter (SerT). In pharmacological assays, the IC₅₀ of SERT-IN-3 for SerT was 34.4 nM .

HY-103194

KH7 5 Publications Verification	Adenylate Cyclase	Others
KH7 is a soluble adenylyl cyclase (sAC)-specific inhibitor, with IC₅₀s of 3-10 μM toward both recombinant purified human sAC_t protein and heterologously expressed sACt in cellular assays . KH7 is also a cAMP inhibitor .

HY-13208

Xevinapant hydrochloride 5+ Cited Publications AT-406 hydrochloride; Debio 1143 hydrochloride; SM-406 hydrochloride	IAP Apoptosis	Cancer
Xevinapant (AT-406) hydrochloride is a potent and orally bioavailable Smac mimetic and an antagonist of the inhibitor of apoptosis proteins (IAPs). Xevinapant hydrochloride binds to XIAP, cIAP1, and cIAP2 proteins with K_is of 66.4, 1.9, and 5.1 nM, respectively. Xevinapant hydrochloride effectively antagonizes XIAP BIR3 protein in a cell-free functional assay, induces rapid degradation of cellular cIAP1 protein, and inhibits cancer cell growth in various human cancer cell lines. Xevinapant hydrochloride is highly effective in induction of apoptosis in xenograft tumors .

HY-13271B

Tubastatin A TFA TSA TFA	HDAC Autophagy Apoptosis	Cancer
Tubastatin A (TSA) TFA is a potent and selective?HDAC6?inhibitor with?IC₅₀?of 15 nM in a cell-free assay, and is selective (1000-fold more) against all other isozymes except HDAC8 (57-fold more). Tubastatin A TFA also inhibits HDAC10 and *metallo-β-lactamase domain-containing protein?2 (MBLAC2)*.

HY-153437

RIP1 kinase inhibitor 7	RIP kinase	Cancer
RIP1 kinase inhibitor 7 (compound 41) is a potent *receptor interacting protein* 1 kinase (RIP1) Inhibitor with an IC₅₀** of <100 nM for human RIP1. RIP1 kinase inhibitor 7 has an EC₅₀ of 1-100 nM in the cell necrosis assay .

HY-19767

GSK 3008348 2 Publications Verification	Integrin	Cardiovascular Disease
GSK 3008348 is a small molecule integrin αvβ6 antagonist, extracted from patent WO/2014154725A1, example 1. The affinity (pIC50) for the human - a 6 protein in the Fluorescence Polarisation Assay for Integrin Antagonist 1 was 8.1, whereas its affinity in the cell Adhesion Assays was for: ανβ6 (pIC50 = 8.4); ανβ3 (pIC50 = 6); ανβ5 (pIC50 = 6.9); ανβ8 (pIC50 = 7.7).

HY-19767A

GSK 3008348 hydrochloride 2 Publications Verification	Integrin	Cardiovascular Disease
GSK 3008348 hydrochloride is a small molecule integrin αvβ6 antagonist, extracted from patent WO/2014154725A1, example 1. The affinity (pIC50) for the human - a 6 protein in the Fluorescence Polarisation Assay for GSK 3008348 (hydrochloride) is 8.1, whereas its affinity in the cell Adhesion Assays was for: ανβ6 (pIC50 = 8.4); ανβ3 (pIC50 = 6); ανβ5 (pIC50 = 6.9); ανβ8 (pIC50 = 7.7).

HY-14460

AM679	FLAP	Inflammation/Immunology
AM679 is a potent, selective *5-lipoxygenase-activating protein* (FLAP) inhibitor with an IC₅₀** of 2 nM in a human FLAP membrane binding assay. AM679 markedly reduces the respiratory syncytial virus-driven ocular pathology as well as the synthesis of cysteinyl leukotrienes (CysLTs) in the eye .

HY-120179A

LP-922761 hydrate	AAK1	Neurological Disease
LP-922761 hydrate is a potent, selective and orally active *adapter protein-2 associated kinase 1 (AAK1) inhibitor with IC₅₀s of 4.8 nM and 7.6 nM in enzyme and cell assays, respectively. LP-922761 hydrate also inhibits BMP-2-inducible protein* kinase (BIKE) with an IC₅₀** of 24 nM. LP-922761 hydrate shows less activity at cyclin G-associated kinase (GAK), opioid, adrenergic α2 or GABAa receptors .

Cat. No.	Product Name	Type

HY-15941

5(6)-FITC

2 Publications Verification

Fluorescein 5(6)-isothiocyanate; Fluorescein isothiocyanate 5- and 6- isomers

Fluorescent Dyes/Probes

5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is an amine-reactive derivative of a fluorescent dye, characterized by high absorbance and excellent fluorescence quantum yield. The isothiocyanate group of FITC can react with various functional groups on proteins, including amines, thiols, imidazoles, tyrosines and carbonyls, enabling the labeling of proteins such as antibodies and lectins. 5(6)-FITC has a wide range of applications, including flow cytometry, immunofluorescence, protease assays and conjugation. The maximum excitation/emission wavelengths are 492/518 nm .

HY-D0018

DCIP sodium

Dichlorophenylindophenol sodium; DCPIP sodium; Indochlorophenol sodium

Dyes

DCIP sodium is a blue dye commonly used in various biochemical and biotechnological applications as an indicator of redox reactions. DCIP sodium has unique chemical properties that change color according to the oxidation state of the substance being tested. It is commonly used in enzyme assays, such as measuring the activity of succinate dehydrogenase, or in protein quantification methods, such as the Lowry assay.

HY-D1809

Vari Fluor 405-Streptavidin

Dyes

Vari Fluor 405-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=405 nm/431 nm.

HY-D1805

Vari Fluor 647-Streptavidin

Dyes

Vari Fluor 647-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=650 nm/665 nm.

HY-D1808

Vari Fluor 488-Streptavidin

Dyes

Vari Fluor 488-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=490 nm/515 nm.

HY-D0018R

DCIP (sodium) (Standard)

Dyes

DCIP (sodium) (Standard) is the analytical standard of DCIP (sodium). This product is intended for research and analytical applications. DCIP sodium is a blue dye commonly used in various biochemical and biotechnological applications as an indicator of redox reactions. DCIP sodium has unique chemical properties that change color according to the oxidation state of the substance being tested. It is commonly used in enzyme assays, such as measuring the activity of succinate dehydrogenase, or in protein quantification methods, such as the Lowry assay.

HY-D1654

BDP 581/591 maleimide	Fluorescent Dyes/Probes
BDP 581/591 maleimide is a linker of the BDP 581/591 dye. It has a long fluorescence lifetime and can be used for fluorescence polarization assays. The maleimide group can react with thiol groups to form thioester bonds between pH 6.5 to 7.5, for the labeling of sulfhydryl groups of proteins and peptides.

HY-101974

Biotin-PEG3-Bromide

Fluorescent Dyes/Probes

Biotin-PEG3-Bromide is a short PEG linker featuring a biotin group and a bromide. The bromide is a halogen which is easily displaced by nucleophiles such as alcohols or amines. Alternatively, bromide can be applied in a number of cross-coupling reactions such as in a Suzuki reaction. Biotin is useful for affinity-based applications such as pull-down assays or for ligating with streptavidin proteins.

HY-125452

DiSBAC10

Fluorescent Dyes/Probes

DiSBAC10 is a voltage-sensitive fluorescent probe used to study cell membrane electrical activity in FRET assays. In a resting polarized cell, DiSBAC10 resides on the outer leaflet of the membrane where it accepts photons from excited fluorescein-labeled proteins and re-emits the photons at a higher wavelength. Depolarization of the cell causes rapid translocation of DiSBAC10 to the inner leaflet of the membrane, thereby increasing the distance between fluorophores and reducing the FRET signal.

HY-13271AG

Tubastatin A (GMP)

Fluorescent Dye

Tubastatin A (GMP) is the Tubastatin A (HY-13271A) produced by using GMP guidelines. GMP small molecules work appropriately as an auxiliary reagent for cell therapy manufacture. Tubastatin A is a potent and selective HDAC6 inhibitor with an IC₅₀ of 15 nM in a cell-free assay, and is selective (1000-fold more) against all other isozymes except HDAC8 (57-fold more). Tubastatin A also inhibits HDAC10 and metallo-β-lactamase domain-containing protein 2 (MBLAC2).

HY-D1804

Vari Fluor 680-Streptavidin

Dyes

Vari Fluor 680-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=680 nm/701 nm.

HY-D1807

Vari Fluor 555-Streptavidin

Dyes

Vari Fluor 555-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=555 nm/565 nm.

HY-D1806

Vari Fluor 594-Streptavidin

Dyes

Vari Fluor 594-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=590 nm/617 nm.

Cat. No.	Product Name	Type

HY-Y0537C

Potassium chloride, ≥99.99% trace metals basis Potassium chloride, suitable for the extraction and solubilization of Proteins	Buffer Reagents
Potassium chloride, ≥99.99% trace metals basis is a biochemical assay reagent suitable for protein extraction and solubilization.

HY-141572

1,2-Dioleoyl-sn-glycerol

sn-1,2-Dioleoylglycerol

Drug Delivery

ALN29882 is a glycerolipid located on the plasma membrane. It consists of two fatty acid chains covalently linked to a single glycerol molecule by means of an ester bond. 18:1 DG has been used as a source of diacylglycerol in the diacylglycerol O-acyltransferase 1 (DGAT1) assay. It is also used as a substrate in the DGAT-1 enzyme assay to evaluate compounds as potential inhibitors of DGAT-1. Suitable for lipoprotein overlay screening assays with the recombinant protein His-AtROP6.

HY-15917A

L-Dithiothreitol

1 Publications Verification

Carbohydrates

L-Dithiothreitol (DTT) is a reducing agent commonly used in various biochemical applications to break disulfide bonds in proteins, thereby denaturing proteins or preventing the formation of unwanted aggregates. DTT has a unique chemical property that cleaves the sulfur-sulfur bond in the disulfide bond to form a sulfhydryl group. This makes it a useful tool for protein purification, enzyme assays, and protein structure studies.

HY-W437745

Desthiobiotin-NHS ester	Biochemical Assay Reagents
Desthiobiotin-NHS ester is a biotinylated biochemical assay reagent that reacts with amine groups (such as lysine residues) of proteins or other biomolecules to form stable amide bonds. Desthiobiotin-NHS ester can be used as probes for metalloproteins .

HY-153232

eGFP circRNA-LNP

Drug Delivery

eGFP circRNA-LNP is a lipid nanoparticle (LNP) containing eGFP circRNA, suitable for assays of RNA delivery, translation efficiency, cell viability, etc. eGFP circRNA carries Enhanced Green Fluorescent Protein (Enhanced Green Fluorescent Protein) eGFP, which will express green fluorescent protein after entering the cell. eGFP is commonly used as a reporter gene detectable by fluorescence microscopy or flow cytometry .

HY-D0014

Brilliant blue G-250

3 Publications Verification

Indicators

Brilliant Blue G-250 is a dye commonly used for the visualization of proteins separated by SDS-PAGE, offering a simple staining procedure and high quantitation. In the Bradford protein assay, protein concentrations are determined by the absorbance at 595 nm due to the binding of Brilliant Blue G-250 to proteins. Brilliant Blue G-250 is a safe highly selective P2×7R antagonist with promising consequent inactivation of NLRP3 inflammasome .

HY-116022A

4-Nitrophenyl phosphate disodium hexahydrate 2 Publications Verification p-Nitrophenyl phosphate disodium hexahydrate	Enzyme Substrates
4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm .

HY-15906

AMPPD

1 Publications Verification

Lumi-Phos Plus; Lumigen PPD; PPD

Enzyme Substrates

AMPPD (Lumi-Phos Plus; Lumigen PPD) is a chemiluminescent substrate for alkaline phosphatase (APase). AMPPD is hydrolyzed by APase to generate an unstable dioxetane intermediate, and the intermediate releases a chemiluminescent signal when it decomposes. The luminescent signal of AMPPD can be detected by highly sensitive equipment, thereby achieving quantitative analysis of the target molecule. AMPPD can be used in ultrasensitive enzyme-linked immunosorbent assays (such as quantitative detection of human tissue kininogen), chemiluminescent detection of proteins and nucleic acids, and other fields .

HY-157919

Biotin-PEAC5-maleimide hydrochloride N-6-(Biotinylamino)hexanoyl-N'-[2-(N-maleimido)ethyl]piperazine, hydrochloride	Biochemical Assay Reagents
Biotin-PEAC5-maleimide hydrochloride is a biochemical assay reagent, and can be used in biotin-PEAC5-maleimide (BPM)-labeling assay to assess modification of protein thiols by electrophiles .

HY-153230

Firefly luciferase circRNA-LNP

Drug Delivery

Firefly luciferase circRNA-LNP is a lipid nanoparticle (LNP) containing Firefly luciferase circRNA, suitable for assays of RNA delivery, translation efficiency, cell viability, etc. Luciferase is commonly used as a bioluminescent reporter gene for gene regulation and function studies. Firefly Luciferase circRNA will express firefly luciferase protein after entering the cells, which is often used for promoter activity detection or dual fluorescent molecular complementation experiments. Common luciferase are firefly luciferase and sea kidney luciferase .

HY-13271AG

Tubastatin A (GMP)

Biochemical Assay Reagents

Cat. No.	Product Name	Target	Research Area

HY-P5426

Abl protein tyrosine kinase substrate	Bcr-Abl	Others
Abl protein tyrosine kinase substrate is a biological active peptide. (Abltide is a peptide substrate for Abl Kinase (Abl protein tyrosine kinase), a partner in the gag-Abl fusion protein of the Abelson murine leukemia virus. Used in Western blot and kinase assay.)

HY-P0225

Autocamtide 2 Autocamtide II	CaMK Autophagy	Neurological Disease
Autocamtide 2 is a highly selective peptide substrate of calcium/calmodulin-dependent protein kinase II (CaMKII). It can be used in the CaMKII activity assay.

HY-P1821A

Myelin Basic Protein TFA 2 Publications Verification MHP4-14 TFA	PKC	Neurological Disease
Myelin Basic Protein (MHP4-14) TFA, a synthetic peptide comprising residues 4-14 of myelin basic protein, is a very selective PKC substrate (K_m=7 μM). Myelin Basic Protein TFA is not phosphorylated by cyclic AMP-dependent protein kinase, casein kinases I and II, Ca ²⁺/calmodulin-dependent protein kinase II, or phosphorylase kinase, and can be routinely used for the assay of protein kinase C with low background in the crude tissue extracts .

HY-P1821

Myelin Basic Protein 2 Publications Verification MHP4-14	PKC	Neurological Disease
Myelin Basic Protein (MHP4-14), a synthetic peptide comprising residues 4-14 of myelin basic protein, is a very selective PKC substrate (K_m=7 μM). Myelin Basic Protein is not phosphorylated by cyclic AMP-dependent protein kinase, casein kinases I and II, Ca ²⁺/calmodulin-dependent protein kinase II, or phosphorylase kinase, and can be routinely used for the assay of protein kinase C with low background in the crude tissue extracts .

HY-P1376A

G-Protein antagonist peptide TFA	mAChR Adrenergic Receptor	Endocrinology
G-Protein antagonist peptide TFA is a truncated substance P-related peptide, competes with receptor for G protein binding. G-Protein antagonist peptide TFA inhibits the activation of G_i or G_o by M2 muscarinic cholinergic receptor (M2 mAChR) or of G_s by beta-adrenergic receptor in the reconstituted phospholipid vesicles, assayed by receptor-promoted GTP hydrolysis .

HY-P2556

Histone H3 (21-44)	Peptides	Others
Histone H3 (21-44), derived from histone H3 21-44 amino acids, is usually used as a substrate (such as protein arginine methyltransferases) for methylation assays .

HY-P3940

Ac-MBP (4-14) Peptide	PKC	Others
Ac-MBP (4-14) Peptide is an acetylated MBP (4-14) peptide. MBP (4-14) Peptide is a very selective (protein kinase C) PKC substrate. Ac-MBP (4-14) Peptide can be used for PKC assay in extracts without prior purification to eliminate interfering protein kinases or phosphatases .

HY-P10845

KRL74	HIV	Infection
KRL74 is a cyclic peptide inhibitor for interaction between the p6 domain of the HIV Gag protein and the UEV domain of the human TSG101 protein(p6/UEV) with an IC₅₀ of 5.44 μM and a K_d of 11.9 μM. KRL74 inhibits the budding process of HIV from host cells with an IC₅₀ of 2 μM in virus-like particle (VLP) budding assay .

HY-P3904

P34cdc2 Kinase Substrate Peptide	Biochemical Assay Reagents	Others
P34cdc2 Kinase Substrate Peptide is a synthetic peptide substrates for P34 ^cdc2 kinase, with a K_m of 74 μM for the purified human enzyme. P34cdc2 Kinase Substrate Peptide can be used in a rapid assay of P34 ^cdc2 phosphorylation in vitro. P34 ^cdc2, a protein serinehhreonine kinase, is a cell division cycle-regulated protein kinase .

HY-P5510

Ac-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu-ψ-(COO)Ala-Ser-Lys(DABCYL)-NH2 HCV NS3 protease substrate	Peptides	Others
Ac-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu-ψ-(COO)Ala-Ser-Lys(DABCYL)-NH2 (HCV NS3 protease substrate) is a biological active peptide. (This peptide is a HCV protease substrate incorporating an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM.

HY-P5429

DNA-PK Substrate	Peptides	Others
DNA-PK Substrate is a biological active peptide. (A substrate for DNA-dependent protein kinase (DNA-PK), phosphorylation. DNA-PK is essential for the repair of DNA double-strand breaks. This peptide corresponding to 11–24 amino acids of human p53 with threonine 18 and serine 20 changed to alanine is used as a substrate for the assay of DNA-PK activityPyroglutamyl (pGlu) peptides may spontaneously form when either Glutamine (Q) or Glutamic acid (E) is located at the sequence N-terminus. The conversion of Q or E to pGlu is a natural occurrence and in general it is believed that the hydrophobic γ-lactam ring of pGlu may play a role in peptide stability against gastrointestinal proteases. Pyroglutamyl peptides are therefore considered a normal subset of such peptides and are included as part of the peptide purity during HPLC analysis.)

HY-P5380

TNO211 Dabcyl-GABA-Pro-Gln-Gly-Leu-Glu(EDANS)-Ala-Lys-NH2	MMP	Others
TNO211 is a biological active peptide. (Matrix Metalloproteinases (MMPs) are a large family of endopeptidases. Collectively, MMPs can degrade all kinds of extracellular matrix proteins, and can also process a number of bioactive molecules. They are known to be involved in the cleavage of cell surface receptors, the release of apoptotic ligands, and chemokine/cytokine inactivation. MMPs are also thought to play a major role in cell behaviors such as cell proliferation, migration (adhesion/dispersion), differentiation, angiogenesis, apoptosis, and host defense.This peptide is a highly soluble fluorogenic MMP substrate for MMP-2, 8, 12, 13 and 14, containing the MMP cleavable Gly-Leu bond and EDANS/DABCYL. Fluorogenic assays using TNO211 are sensitive and can detect MMP activity in culture medium from endothelial cells and untreated synovial fluid from patients. Abs/Em = 340/490 nm.)

HY-P5372

Ala-parafluoroPhe-Arg-Cha-Cit-Tyr-NH2	Protease Activated Receptor (PAR)	Others
Ala-parafluoroPhe-Arg-Cha-Cit-Tyr-NH2 is a biological active peptide. (Protease activated receptor 1 (PAR-1) belongs to a subfamily of G-protein coupled receptors and is known to mediate the cellular effects of thrombin. This peptide is a PAR-1 selective agonist displaying a high level of specificity to PAR-1 over PAR-2. The specificity of peptide was evaluated in cell-based calcium signaling assay using HEK293 cells. PAR-1 selective agonists can be used to study PAR-1 activation in vivo. In addition to its varied cellular effects of thrombin, PAR-1 has also been shown to coordinate with PAR-4 and regulate thrombin-induced hepatocellular carcinoma harboring thrombin formation within the tumor environment classified as 'coagulation type'.)

Cat. No.	Product Name

HY-K0016

Kinase Assay Buffer
MCE Kinase Assay Buffer can be used for assaying kinase protein.

HY-K2001

*Bradford Protein* Assay Kit**
MCE Bradford Protein Assay Kit is suitable for detecting the total protein content in samples such as serum, plasma, animal tissues, and cells.

HY-K0235

Anti-YFP Affinity Gel
MCE Anti-YFP Affinity Gel can be used for the detection and purification of proteins tagged with GFP and EGFP and IP assays.

HY-K0229

Anti-GFP Affinity Gel
MCE Anti-GFP Affinity Gel can be used for the detection and purification of GFP, EGFP, their fusion-expressed proteins and IP assays.

HY-K0237

Anti-HA Magnetic Agarose Beads
MCE Anti-HA Magnetic Agarose Beads can be used for the detection and purification of HA fusion-expressed proteins and IP assays.

HY-K0236

Anti-Flag Magnetic Agarose Beads
MCE Anti-GFP Magnetic Agarose Beads can be used for the detection and purification of Flag (DYKDDDDK) fusion-expressed proteins and IP assays.

HY-K0246

Anti-GFP Magnetic Agarose Beads
MCE Anti-GFP Magnetic Agarose Beads can be used for the detection and purification of GFP, EGFP, and their fusion-expressed proteins and IP assays.

HY-K1000

WB/IP Lysis Buffer
MCE WB/IP Lysis Buffer is a lysis buffer used to lyse cell or tissue samples under non-denaturing conditions to prepare protein samples. The lysed cell or tissue samples can be used for PAGE, Western Blot, Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), ELISA, and other assays.

Cat. No.	Product Name	Category	Target	Chemical Structure

HY-W014502

D-Kynurenine	Structural Classification Classification of Application Fields Ketones, Aldehydes, Acids Endogenous metabolite Disease Research Fields Cancer	Aryl Hydrocarbon Receptor Endogenous Metabolite
D-kynurenine, a metabolite of D-tryptophan, can serve as the bioprecursor of kynurenic acid (KYNA) and 3-hydroxykynurenine. D-Kynurenine is an agonist for G protein-coupled receptor, GPR109B. D-Kynurenine is a substrate in a fluorometric assay of D-amino acid oxidase. D-kynurenine promotes epithelial-to-mesenchymal transition via activating aryl hydrocarbon receptor (AHR) .

HY-N2853R

D-α-Tocopherylquinone (Standard)	Quinones Microorganisms Source classification Benzene Quinones	Others
DCIP (sodium) (Standard) is the analytical standard of DCIP (sodium). This product is intended for research and analytical applications. DCIP sodium is a blue dye commonly used in various biochemical and biotechnological applications as an indicator of redox reactions. DCIP sodium has unique chemical properties that change color according to the oxidation state of the substance being tested. It is commonly used in enzyme assays, such as measuring the activity of succinate dehydrogenase, or in protein quantification methods, such as the Lowry assay.

HY-W014502R

D-Kynurenine (Standard)	Structural Classification Ketones, Aldehydes, Acids Endogenous metabolite	Aryl Hydrocarbon Receptor Endogenous Metabolite
D-Kynurenine (Standard) is the analytical standard of D-Kynurenine. This product is intended for research and analytical applications. D-kynurenine, a metabolite of D-tryptophan, can serve as the bioprecursor of kynurenic acid (KYNA) and 3-hydroxykynurenine. D-Kynurenine is an agonist for G protein-coupled receptor, GPR109B. D-Kynurenine is a substrate in a fluorometric assay of D-amino acid oxidase. D-kynurenine promotes epithelial-to-mesenchymal transition via activating aryl hydrocarbon receptor (AHR) .

Cat. No.	Product Name		Classification

HY-134813

MRTX1133 20+ Cited Publications		Alkynes
MRTX1133 is a noncovalent, potent, and selective alkyne-based KRAS G12D inhibitor. MRTX1133 optimally fills the switch II pocket and extends three substituents to favorably interact with the protein, resulting in an estimated K_D against KRAS G12D of 0.2 pM. MRTX1133 prevents SOS1-catalyzed nucleotide exchange and/or formation of the KRAS G12D/GTP/RAF1 complex, thereby inhibiting mutant KRAS-dependent signal transduction. MRTX1133 selectively inhibits KRAS G12D mutant, but not KRAS wild-type, tumor cells. MRTX1133 has single digit nanomolar activity in cellular assays and marked in vivo efficacy in tumor models harboring KRAS G12D mutations .

HY-158385

5-Azido-PEG4-SS-UTP tristriethylamine		Azide
5-Azido-PEG4-SS-UTP tristriethylamine is a biochemical assay reagent, which can be used in click chemistry. Click chemistry has great potential for use in binding between nucleic acids, lipids, proteins, and other molecules, and has been used in many research fields because of its beneficial characteristics, including high yield, high specificity, and simplicity .

Cat. No.	Product Name		Classification

HY-153080

Firefly Luciferase mRNA(N1-Me-Pseudo UTP)		mRNA
Firefly luciferase mRNA will express a luciferase protein, originally isolated from the Photinus pyralis (Firefly). Firefly Luciferase is a frequently used bioluminescent reporter for gene regulation and function study. It is applicable in assays for gene expression, cell viability and in vivo imaging etc.

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