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Results for "

FRET

" in MedChemExpress (MCE) Product Catalog:

By Targets:	Androgen Receptor (1) Angiotensin Receptor (1) Atg4 (1) Autophagy (3) Bcl-2 Family (3) Beta-secretase (1) Biochemical Assay Reagents (5) C-type Lectin-like Receptors (CTLRs) (2) Cathepsin (3) Cholinesterase (ChE) (1) CMV (1) DNA Stain (1) Epigenetic Reader Domain (4) Fluorescent Dye (24) FXR (4) GLP Receptor (1) Histone Acetyltransferase (4) Histone Demethylase (1) Histone Methyltransferase (1) HIV (1) Isotope-Labeled Compounds (1) Keap1-Nrf2 (2) Ligands for E3 Ligase (1) Lipocalin Family (1) Liposome (1) Mitophagy (1) Parasite (1) ROR (1) SARS-CoV (3) Ser/Thr Protease (1) STING (1) Tau Protein (1) Virus Protease (1) YAP (1)
By Research Areas:	Cancer (15) Infection (6) Inflammation/Immunology (9) Metabolic Disease (8) Neurological Disease (2)
Click Chemistry:	DBCO (1) Alkynes (1)
Oligonucleotides:	Fluorescent Lipids (1) Pegylated Lipids (1)

Inhibitors & Agonists

Fluorescent Dye

Biochemical Assay Reagents

Peptides

Inhibitory Antibodies

Isotope-Labeled Compounds

Click Chemistry

Oligonucleotides

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-P2498A

*Cathepsin D and E FRET* Substrate acetate**	Cathepsin	Cancer
Cathepsin D and E FRET Substrate acetate is a fluorogenic substrate for cathepsins D and E and not for B, H or L. The cleavage occurs at the Phe-Phe amide bond resul. Cathepsin D and E FRET Substrate is a valuable tool for routine assays and for mechanistic studies on cathepsins E and D .

HY-P3792

Mca-Pro-Leu-Gly-Pro-D-Lys(Dnp)	Biochemical Assay Reagents	Others
Mca-Pro-Leu-Gly-Pro-D-Lys(Dnp) is a FRET substrate of Thimet oligopeptidase. Mca-Pro-Leu-Gly-Pro-D-Lys(Dnp) can be used for the determination of Thimet oligopeptidase activity .

HY-D2365

QSY 21 NHS	Fluorescent Dye	Others
QSY 21 NHS, a dark quencher is an efficient energy transfer acceptor of the far red and NIR fluorescent probes. QSY 21 NHS works in the wavelength range of 540-750 nm, and is frequently used in FRET applications. QSY 21 NHS does not emit fluorescence in normal conditions. NHS esters can be used to label the primary amines (R-NH₂) of proteins, amine-modified oligonucleotides, and other amine-containing molecules .

HY-P5354

FRETS-VWF73	Fluorescent Dye	Others
FRETS-VWF73, a 73-amino-acid peptide, is a fluorogenic substrate for ADAMTS13 assay (Ex=340 nm; Em=450 nm). .

HY-P2492

*Renin FRET* Substrate I**	Angiotensin Receptor	Metabolic Disease
Renin FRET Substrate I is a substrate of human renin. Renin FRET Substrate I is designed to incorporate the renin cleavage site that occurs in the N-terminal peptide of human angiotensinogen .

HY-P2498

*Cathepsin D and E FRET* Substrate**	Cathepsin	Others
Cathepsin D and E FRET Substrate is a fluorogenic substrate for cathepsins D and E and not for B, H or L. The cleavage occurs at the Phe-Phe amide bond resul. Cathepsin D and E FRET Substrate is a valuable tool for routine assays and for mechanistic studies on cathepsins E and D .

HY-161352

NPI52	Virus Protease	Infection
NPI52 (Compound E1) is a foot-and-mouth disease virus 3C protease (FMDV 3Cpro) inhibitor, with IC₅₀ values of 0.05 and 0.11 µM in FRET and cell-based assay, respectively .

HY-P10344

MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2	Fluorescent Dye	Infection
MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 is a fluorescent substrate used for fluorescence resonance energy transfer (FRET) protease assays. This substrate is employed to measure the activity of SARS-CoV-2 3CLpro .

HY-160276

DOPE-PEG-Fluor 555,MW 5000	Biochemical Assay Reagents Fluorescent Dye Liposome	Others
DOPE-PEG-Fluor 555, MW 5,000 is consist of a DOPE phospholipid which is an unsaturated phospholipid and a Fluor 555 dye which is a bright orange cyanine dye that can be used in fluorescence microscopy, FRET and other in vivo imaging techniques.

HY-160943A

F594-1001 hydrochloride	SARS-CoV	Infection
F594-1001 (compound 6) hydrochloride is a potent and highly selective SARS-CoV-2 Mac1-ADP-ribose inhibitor with IC₅₀s of 8.5 μM, 68 μM and 45 μM for SARS-CoV-2 in AS, FP, and FRET assays, respectively. F594-1001 hydrochloride directly binds to SARS-CoV-2 Mac1 and exhibits a dose-dependent inhibition of Mac1 ADP-ribosylhydrolase activity .

HY-160943

F594-1001	SARS-CoV	Infection
F594-1001 (compound 6) is a potent and highly selective SARS-CoV-2 Mac1-ADP-ribose inhibitor with IC₅₀s of 8.5 μM, 68 μM and 45 μM for SARS-CoV-2 in AS, FP, and FRET assays, respectively. F594-1001 directly binds to SARS-CoV-2 Mac1 and exhibits a dose-dependent inhibition of Mac1 ADP-ribosylhydrolase activity .

HY-W027544

MCA 7-Methoxycoumarin-4-acetic acid	Fluorescent Dye	Others
MCA (7-Methoxycoumarin-4-acetic acid) is a Coumarin (HY-N0709) derivative. MCA quantitates platelet-activating factor (PAF) by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities .

HY-144127

AR antagonist 3	Androgen Receptor	Cancer
AR antagonist 3 is a potent and selective androgen receptor (AR) antagonist with an IC₅₀ of 0.47 µM. AR antagonist 3 exhibits a dose-dependent decrease of the FRET signal (IC₅₀= 18.05 μM). AR antagonist 3 shows effective inhibition on tumor growth when administered intratumorally .

HY-P1859

MCA-SEVNLDAEFR-K(Dnp)-RR, amide	Fluorescent Dye	Others
MCA-SEVNLDAEFR-K(Dnp)-RR, amide is a FRET-based substrate.

HY-102075A

TMP778	ROR	Inflammation/Immunology
TMP778 is a potent and selevtive RORγt inverse agonist, with an IC₅₀ of 7 nM in FRET assay.

HY-151412

T-271	Mitophagy	Neurological Disease
T-271 is a selective inducer of mitochondrial autophagy .

HY-100522

FMK 9a 2 Publications Verification	Atg4 Autophagy	Cancer
FMK 9a is an autophagin-1 inhibitor with IC₅₀ values of 80 and 73 μM in FRET and LRA assay.

HY-147366

RBP4 ligand-1	Lipocalin Family	Metabolic Disease
RBP4 ligand-1 is a non-retinoid ligands for (RBP4) retinol-binding protein 4. The hRBP4_SPA IC₅₀ and *RBP4-TTR FRET* IC₅₀** values are 0.23±0.11 and 0.13±0.12 μM in the radioligand binding assay and FRET assay, respectively .

HY-D1682

NFQ1	Fluorescent Dye	Others
NFQ1 is a non-fluorescent quencher (absorption wavelength: 474 nm), and is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method. OS-FRET enable measurement of unquenched donor emission in the same sample. NFQ1 shows broad absorption spectrum ensuring its utility as a dark acceptor for many donors .

HY-D1046

Dabcyl acid, SE Dabcyl, SE	Fluorescent Dye	Metabolic Disease
Dabcyl acid, SE is the amino-reactive form of Dabcyl acid (DABCYL), and widely used to prepare a variety of FRET-based probes that contain DABCYL.

HY-101533A

AZD-5991 Racemate	Bcl-2 Family	Cancer
AZD-5991 Racemate is the racemate of AZD-5991. AZD-5991 Racemate is a Mcl-1 inhibitor with an IC₅₀ of <3 nM in FRET assay.

HY-W998662

Coumarin 343 X carboxylic acid	Fluorescent Dye	Others
Coumarin 343 X carboxylic acid is a blue emitting fluorophore used as a laser dye. The fluorophore can serve as a FRET donor for FAM (fluorescein).

HY-100443B

(R,R)-PX20606	FXR Autophagy	Metabolic Disease
(R,R)-PX20606 is a FXR agonist with EC₅₀s of 18 and 29 nM for FXR in FRET and M1H assay, respectively.

HY-125452

DiSBAC10	Fluorescent Dye	Others
DiSBAC10 is a voltage-sensitive fluorescent probe used to study cell membrane electrical activity in FRET assays. In a resting polarized cell, DiSBAC10 resides on the outer leaflet of the membrane where it accepts photons from excited fluorescein-labeled proteins and re-emits the photons at a higher wavelength. Depolarization of the cell causes rapid translocation of DiSBAC10 to the inner leaflet of the membrane, thereby increasing the distance between fluorophores and reducing the FRET signal.

HY-101533

AZD-5991 Maximum Cited Publications 12 Publications Verification	Bcl-2 Family	Cancer
AZD-5991 is a potent and selective Mcl-1 inhibitor with an IC₅₀ of 0.7 nM in FRET assay and a K_d of 0.17 nM in surface plasmon resonance (SPR) assay .

HY-100443A

trans-PX20606 1 Publications Verification trans-PX-102	FXR Autophagy	Metabolic Disease
PX20606 trans racemate (PX-102 trans racemate) is a FXR agonist with EC₅₀s of 32 and 34 nM for FXR in FRET and M1H assay, respectively .

HY-D2274

EZH2-AF647	Fluorescent Dye	Others
EZH2-AF647 is a fluorescent probe derived from UNC2239 that improves receptor TR-FRET properties by using Alexa 647 dye .

HY-101027

GSK 4027 1 Publications Verification	Epigenetic Reader Domain Histone Acetyltransferase	Inflammation/Immunology Cancer
GSK 4027 is a chemical probe for the PCAF/GCN5 bromodomain with an pIC₅₀ of 7.4±0.11 for PCAF in a time-resolved fluorescence resonance energy transfer (TR-FRET) assay.

HY-148643

STING modulator-5	STING	Inflammation/Immunology
STING modulator-5 (compound 38) is a STING modulator with a pIC₅₀ value of 9.5. STING modulator-5 antagonizes peripheral blood mononuclear cells (PBMC) with a pIC₅₀ value of 8.1. STING modulator-5 is a antagonist of THP-1 cells, it can be used for immunological disease research .

HY-D0053A

6-ROX hydrochloride 6-Carboxy-X-rhodamine hydrochloride	Fluorescent Dye	Others
6-ROX (6-Carboxy-X-rhodamine) hydrochloride, a fluorescent marker of oligonucleotides, acts as a receptor coupled to 5-FAM and as a donor in FRET imaging. Excitation wavelength: 568 nm. Emission wavelength: 568 nm.

HY-168536

SJ46411	Others	Cancer
SJ46411 is a potent ligand for KLHDC2, with a K_d of ~260 nM measured by isothermal titration calorimetry (SPR K_d = 0.5 μM, TR-FRET IC₅₀ = 3.9 μM, ΔTm = 6.0 °C) .

HY-149233

hAChE-IN-1	Cholinesterase (ChE) Tau Protein	Neurological Disease
hAChE-IN-1 (Compound 24) is a potent hAChE inhibitor with an IC₅₀ of 1.09 μM. hAChE-IN-1 inhibits tau-oligomerization with an EC₅₀ of 2.71 μM in cellular tau FRET assay .

HY-161159

DMHBO+	Fluorescent Dye	Others
DMHBO+ is a cationic chromophore that binds to the Chili aptamer with a K_d of 12 nM. The Chili-DMHBO+ complex is an ideal fluorescence donor for FRET to the rhodamine dye Atto 590, suitable for imaging RNA in cells. Ex/Em=456/592 nm .

HY-D1079

EDANS sodium	DNA Stain	Others
EDANS sodium is a potent fluorogenic substrates. EDANS sodium is a donor for FRET-based nucleic acid probes and protease substrates. EDANS sodium is often paired with DABCYL or DABSYL. The optimal absorbance and emission wavelengths of EDANS sodium are λ_abs = 336 nm and λ_em = 490 nm respectively .

HY-P4787

Ac-Glu-Asp(EDANS)-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Gly-Lys(DABCYL)-Glu-NH2	Cathepsin	Others
Ac-Glu-Asp(EDANS)-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Gly-Lys(DABCYL)-Glu-NH2 is a cathepsin D substrate, that can be used for cathepsin D FRET assay .

HY-157558

KDRLKZ-1 8SGE	Ligands for E3 Ligase	Others
KDRLKZ-1 is a KLHDC2 ligand (K_d = 0.36 μM), with IC₅₀s of 0.21 μM and 0.31 μM in alphaLISA and time-resolved fluorescence resonance energy transfer (TR-FRET) displacement assays .

HY-101027A

GSK4028 1 Publications Verification	Epigenetic Reader Domain Histone Acetyltransferase	Inflammation/Immunology Cancer
GSK4028 is the enantiomeric negative control of GSK4027, which is a PCAF/GCN5 bromodomain chemical probe, the pIC₅₀ of GSK4028 is 4.9 in a time-resolved fluorescence resonance energy transfer (TR-FRET) assay.

HY-101533B

AZD-5991 (S-enantiomer)	Bcl-2 Family	Cancer
AZD-5991 S-enantiomer is the less active enantiomer of AZD-5991. AZD-5991 S-enantiomer is a Mcl-1 inhibitor with an IC₅₀ of 6.3 μM in FRET assay and a K_d of 0.98 μM in surface plasmon resonance (SPR) assay.

HY-108435

GNE-049 4 Publications Verification	Epigenetic Reader Domain Histone Acetyltransferase	Cancer
GNE-049 is a highly potent and selective CBP inhibitor with an IC₅₀ of 1.1 nM in TR-FRET assay. GNE-049 also inhibits BRET and BRD4(1) with IC₅₀s of 12 nM and 4200 nM, respectively .

HY-D1049

DNP-X acid 6-((2,4-Dinitrophenyl)amino)hexanoic acid	Fluorescent Dye	Metabolic Disease
DNP-X acid (6-((2,4-Dinitrophenyl)amino)hexanoic acid), an amine-reactive building block for developing a probe, can be recognized by anti-DNP antibodies. DNP-X acid is also an excellent amine-reactive FRET quencher paired with Trp or Tyr.

HY-P99900

Sevirumab MSL-109	CMV	Infection
Sevirumab (MSL-109) is a human IgG1 neutralizing monoclonal antibody against cytomegalovirus (CMV). Sevirumab recognizes CMV gH complexes and inhibits CMV replication with an EC₅₀ of 0.3 μg/mL .

HY-173426

SARS-CoV-2 Mpro-IN-40	SARS-CoV	Infection
SARS-CoV-2 Mpro-IN-40 (compound 119) is an inhibitor of SARS-CoV-2 Mpro, with an IC₅₀ value of 15.7 nM against Mpro Fret. SARS-CoV-2 Mpro-IN-40 exhibits low metabolic stability in human microsomes .

HY-172932

TEAD-IN-20	YAP	Cancer
TEAD-IN-20 is a TEAD inhibitor. The IC₅₀ values of TEAD-IN-20 in TEAD4 FRET and TEAD4 MCF7-Tead cell lines are 0.021 and 0.044 μM, respectively. TEAD-IN-20 can be used in cancer research .

HY-149245

Keap1-Nrf2-IN-15	Keap1-Nrf2	Inflammation/Immunology
Keap1-Nrf2-IN-15 (Compound 24a) is a potent Keap1-Nrf2 protein-protein interaction inhibitor with IC₅₀s of 77 nM and 2.5 nM in FP assay and TR-FRET assay, respectively .

HY-139561

KDM2B-IN-2	Histone Demethylase	Inflammation/Immunology Cancer
KDM2B-IN-2, a potent histone demethylase (kdm2b) inhibitor with an IC₅₀ of 0.021 μM in a KDM2B TR-FRET assay. KDM2B-IN-2 can be used for hyperproliferative diseases research .

HY-D2579

Sulfo DBCO-UBQ-2	Biochemical Assay Reagents	Others
Sulfo DBCO-UBQ-2 is a click chemistry reagent combining a dark quencher, UBQ-2, with a polyaromatic-azo backbone, offering no native emission. UBQ-2 effectively quenches fluorescence in the 560-670 nm range, ideal for qPCR probes and FRET applications with orange to far-red dyes .

HY-155311

NKG2D-IN-1	C-type Lectin-like Receptors (CTLRs)	Inflammation/Immunology
NKG2D-IN-1 (compound 21) is a natural killer group 2D receptor (NKG2D) inhibitor with IC₅₀s of 0.3 µM and 0.7 µM in NKG2D/MICA and ULBP6 TR-FRET assays, respectively .

HY-155312

NKG2D-IN-2	C-type Lectin-like Receptors (CTLRs)	Cancer
NKG2D-IN-2 (compound 47) is a natural killer group 2D receptor (NKG2D) inhibitor with IC₅₀s of 0.1 µM and 0.2 µM in NKG2D/MICA and ULBP6 TR-FRET assays, respectively .

HY-D2742

DMT-BH1 amidite BHQ-1 DMT amidite	Fluorescent Dye	Others
DMT-BH1 amidite (BHQ-1 DMT amidite) is a true dark quencher with broad absorption curve which covers the visible spectrum with maximum in green to yellow region. It is used for the synthesis of dual labeled oligonucleotide probes for qPCR bearing 5'-quencher DusQ 1 and other FRET applications for multiplexing assays.

HY-170831

DS79932728	Histone Methyltransferase GLP Receptor	Others
DS79932728 is an orally active inhibitor for G9a and GLP with IC₅₀ of 12.6 nM and 75.7 nM. DS79932728 induces the production of γ-globin, thereby increasing the level of fetal hemoglobin (HbF). DS79932728 increases F-reticulocytes (F-rets) proportion and exhibits good oral absorption characteristics in cynomolgus monkey models .

Cat. No.	Product Name	Type

HY-D2365

QSY 21 NHS

Dyes

QSY 21 NHS, a dark quencher is an efficient energy transfer acceptor of the far red and NIR fluorescent probes. QSY 21 NHS works in the wavelength range of 540-750 nm, and is frequently used in FRET applications. QSY 21 NHS does not emit fluorescence in normal conditions. NHS esters can be used to label the primary amines (R-NH₂) of proteins, amine-modified oligonucleotides, and other amine-containing molecules .

HY-W027544

MCA 7-Methoxycoumarin-4-acetic acid	Fluorescent Dyes/Probes
MCA (7-Methoxycoumarin-4-acetic acid) is a Coumarin (HY-N0709) derivative. MCA quantitates platelet-activating factor (PAF) by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities .

HY-D1046

Dabcyl acid, SE Dabcyl, SE	Fluorescent Dyes/Probes
Dabcyl acid, SE is the amino-reactive form of Dabcyl acid (DABCYL), and widely used to prepare a variety of FRET-based probes that contain DABCYL.

HY-D1050

DNP-X, SE 1 Publications Verification 6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester	Fluorescent Dyes/Probes
DNP-X, SE (6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester), the DNP-X acid modified by succinimidyl ester, is an amine-reactive building block for developing a probe, which can be recognized by anti-DNP antibodies. DNP-X, SE (6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester) is also an excellent amine-reactive FRET quencher paired with Trp or Tyr.

HY-D1682

NFQ1	Fluorescent Dyes/Probes
NFQ1 is a non-fluorescent quencher (absorption wavelength: 474 nm), and is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method. OS-FRET enable measurement of unquenched donor emission in the same sample. NFQ1 shows broad absorption spectrum ensuring its utility as a dark acceptor for many donors .

HY-W998662

Coumarin 343 X carboxylic acid	Fluorescent Dyes/Probes
Coumarin 343 X carboxylic acid is a blue emitting fluorophore used as a laser dye. The fluorophore can serve as a FRET donor for FAM (fluorescein).

HY-125452

DiSBAC10

Fluorescent Dyes/Probes

DiSBAC10 is a voltage-sensitive fluorescent probe used to study cell membrane electrical activity in FRET assays. In a resting polarized cell, DiSBAC10 resides on the outer leaflet of the membrane where it accepts photons from excited fluorescein-labeled proteins and re-emits the photons at a higher wavelength. Depolarization of the cell causes rapid translocation of DiSBAC10 to the inner leaflet of the membrane, thereby increasing the distance between fluorophores and reducing the FRET signal.

HY-D2274

EZH2-AF647	Fluorescent Dyes/Probes
EZH2-AF647 is a fluorescent probe derived from UNC2239 that improves receptor TR-FRET properties by using Alexa 647 dye .

HY-D1049

DNP-X acid 6-((2,4-Dinitrophenyl)amino)hexanoic acid	Fluorescent Dyes/Probes
DNP-X acid (6-((2,4-Dinitrophenyl)amino)hexanoic acid), an amine-reactive building block for developing a probe, can be recognized by anti-DNP antibodies. DNP-X acid is also an excellent amine-reactive FRET quencher paired with Trp or Tyr.

HY-D2579

Sulfo DBCO-UBQ-2	Fluorescent Dyes/Probes
Sulfo DBCO-UBQ-2 is a click chemistry reagent combining a dark quencher, UBQ-2, with a polyaromatic-azo backbone, offering no native emission. UBQ-2 effectively quenches fluorescence in the 560-670 nm range, ideal for qPCR probes and FRET applications with orange to far-red dyes .

HY-D2167

AF 568 alkyne	Fluorescent Dyes/Probes
AF 568 alkyne is a derivative of the orange fluorescent dye AF 568. AF 568 has an absorption wavelength of 590-720 nm (FRET) and an emission wavelength of 515-720 nm. AF 568 alkyne can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) via the Alkyne group and molecules containing Azide groups. To achieve specific coupling of dye labels and biomolecules .

HY-173308

QSY-21

Fluorescent Dyes/Probes

QSY-21 is a fluorescence quencher. QSY-21 possesses broad absorption in far red and NIR range, and can quench fluorescence of dyes that emit in this region. This is a carboxylic acid derivative. QSY-21 has intense absorption maximum at 661 nm, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications. It is a common quencher for Cyanine5, Cyanine5.5, AF 647, or other spectrally similar fluorescent dyes.

HY-D2735

QSY-9-succinimidyl ester

Dyes

QSY-9-succinimidyl ester is a nonfluorescent acceptor molecule with a terminal NHS ester group. QSY-9-succinimidyl ester has a wide and intense quenching range from 500-600 nm that makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with fluorescent dyes at 500 nm to 600 nm. The NHS ester can be applied to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

HY-42984

BHQ-2 NHS

Fluorescent Dyes/Probes

BHQ-2 NHS is a dark quencher with no native emission due to the polyaromatic-azo backbone and a terminal NHS ester. UBHQ-2 NHS has a wide and intense quenching range from 560-670 nm, which makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with orange to far-red emitting dyes. The NHS ester can be applied to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

HY-D2413

BODIPY FL VH032

BODIPY FL PEG4-VH032

Fluorescent Dyes/Probes

BODIPY FL VH032 is a high-affinity VHL fluorescent probe with a K_d value of 3.01 nM. BODIPY FL VH032 consists of von Hippel-Lindau (VHL) ligand VH032 (HY-120217) and BODIPY FL (HY-43520). BODIPY FL VH032 can be used for time-resolved fluorescence resonance energy transfer (TR-FRET) detection for high-throughput identification and characterization of VHL ligands with maximum excitation emission wavelength: 504/520 nm .

HY-D2168

AF 568 azide

Fluorescent Dyes/Probes

AF 568 azide is an azide derivative of the orange fluorescent dye AF 568. AF 568 has an absorption wavelength of 590-720 nm (FRET) and an emission wavelength of 515-720 nm. AF 568 alkyne can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) via the Azide group and molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups. To achieve specific coupling of dye labels and biomolecules .

HY-D2169

AF 568 carboxylic acid

Fluorescent Dyes/Probes

AF 568 carboxylic acid is the non-reactive form of the orange fluorescent dye AF 568. AF 568 has an absorption wavelength of 590-720 nm (FRET) and an emission wavelength of 515-720 nm. AF 568 alkyne forms stable covalent bonds by reacting carboxylic acid groups with molecules bearing amino groups. Copper-catalyzed azide-alkyne cycloaddition (CuAAc) can occur with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups .

Cat. No.	Product Name	Type

HY-160276

DOPE-PEG-Fluor 555,MW 5000	Drug Delivery
DOPE-PEG-Fluor 555, MW 5,000 is consist of a DOPE phospholipid which is an unsaturated phospholipid and a Fluor 555 dye which is a bright orange cyanine dye that can be used in fluorescence microscopy, FRET and other in vivo imaging techniques.

Cat. No.	Product Name	Target	Research Area

HY-P2498A

*Cathepsin D and E FRET* Substrate acetate**	Cathepsin	Cancer
Cathepsin D and E FRET Substrate acetate is a fluorogenic substrate for cathepsins D and E and not for B, H or L. The cleavage occurs at the Phe-Phe amide bond resul. Cathepsin D and E FRET Substrate is a valuable tool for routine assays and for mechanistic studies on cathepsins E and D .

HY-P3792

Mca-Pro-Leu-Gly-Pro-D-Lys(Dnp)	Biochemical Assay Reagents	Others
Mca-Pro-Leu-Gly-Pro-D-Lys(Dnp) is a FRET substrate of Thimet oligopeptidase. Mca-Pro-Leu-Gly-Pro-D-Lys(Dnp) can be used for the determination of Thimet oligopeptidase activity .

HY-P1859

MCA-SEVNLDAEFR-K(Dnp)-RR, amide	Fluorescent Dye	Others
MCA-SEVNLDAEFR-K(Dnp)-RR, amide is a FRET-based substrate.

HY-P5354

FRETS-VWF73	Fluorescent Dye	Others
FRETS-VWF73, a 73-amino-acid peptide, is a fluorogenic substrate for ADAMTS13 assay (Ex=340 nm; Em=450 nm). .

HY-P2492

*Renin FRET* Substrate I**	Angiotensin Receptor	Metabolic Disease
Renin FRET Substrate I is a substrate of human renin. Renin FRET Substrate I is designed to incorporate the renin cleavage site that occurs in the N-terminal peptide of human angiotensinogen .

HY-P2498

*Cathepsin D and E FRET* Substrate**	Cathepsin	Others
Cathepsin D and E FRET Substrate is a fluorogenic substrate for cathepsins D and E and not for B, H or L. The cleavage occurs at the Phe-Phe amide bond resul. Cathepsin D and E FRET Substrate is a valuable tool for routine assays and for mechanistic studies on cathepsins E and D .

HY-P10344

MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2	Fluorescent Dye	Infection
MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 is a fluorescent substrate used for fluorescence resonance energy transfer (FRET) protease assays. This substrate is employed to measure the activity of SARS-CoV-2 3CLpro .

HY-P5970

DABCYL-TNF-α-EDANS (-4 to +6) (human) TACE Substrate	Peptides	Cancer
DABCYL-TNF-α-EDANS (-4 to +6) (human) is a FRET peptide substrate of tumor necrosis factor convertase (TACE) .

HY-P10196

DABCYL-SEVNLDAEF-EDANS	Peptides	Others
DABCYL-SEVNLDAEF-EDANS is a substrate of FRET. DABCYL-SEVNLDAEF-EDANS can be used in the BACE activity assay .

HY-P4787

Ac-Glu-Asp(EDANS)-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Gly-Lys(DABCYL)-Glu-NH2	Cathepsin	Others
Ac-Glu-Asp(EDANS)-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Gly-Lys(DABCYL)-Glu-NH2 is a cathepsin D substrate, that can be used for cathepsin D FRET assay .

HY-P5323

Dabcyl-AGHDAHASET-Edans	Peptides	Others
Dabcyl-AGHDAHASET-Edans is a biological active peptide. (This is a type I signal peptidase (SPase1) substrate peptide labeled with EDANS/ DABCYL FRET pair, and contains a crucial cleavage site derived from the C-terminal region of the Staphylococcus epidermidis pre-SceD protein. Abs/Em = 340/490 nm.)

HY-P5344

Dabcyl-LNKRLLHETQ-Edans Fluorigenic PEXEL peptide	Parasite	Others
Dabcyl-LNKRLLHETQ-Edans (Fluorigenic PEXEL peptide) is a biological active peptide. (This FRET substrate peptide for Plasmepsin V (PMV) is derived from the conserved Plasmodium Export Element (PEXEL) motif of Histidine-Rich Protein II (HRPII). PMV is an ER aspartic protease that recognizes and cleaves the RXL sequence within the PEXEL motif of proteins exported by human malaria parasite Plasmodium falciparum, allowing them to translocate into host erythrocytes.)

HY-P5419

WAAG-3R	Peptides	Others
WAAG-3R is a biological active peptide. (Aggrecanases belong to the ADAMTS (A disintegrin and metalloprotease with thrombospondin motif) family of proteases. Aggrecanases cleave aggrecan, the major structural component of cartilage. Aggrecanase-1 (ADAMTS-4) is a major aggrecanase in human osteoarthritic cartilage. This FRET peptide was used in an ADAMTS-4 (Aggrecanase-1) and ADAMTS-5 (Aggrecanase-2) assay . (Ex/Em = 340/420 nm)

HY-P5419A

WAAG-3R TFA	Biochemical Assay Reagents	Others
WAAG-3R TFA is a biological active peptide. (Aggrecanases belong to the ADAMTS (A disintegrin and metalloprotease with thrombospondin motif) family of proteases. Aggrecanases cleave aggrecan, the major structural component of cartilage. Aggrecanase-1 (ADAMTS-4) is a major aggrecanase in human osteoarthritic cartilage. This FRET peptide was used in an ADAMTS-4 (Aggrecanase-1) and ADAMTS-5 (Aggrecanase-2) assay . (Ex/Em = 340/420 nm)

HY-P5481

DABCYL-LPETG-EDANS	Peptides	Others
DABCYL-LPETG-EDANS is a biological active peptide. (This 5-amino acid peptide is a sortase substrate, C-terminal sorting signal. Sortase cleaves surface proteins at the LPXTG motif and catalyzes the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Sortases are a family of Gram-positive transpeptidases responsible for anchoring surface protein virulence factors to the peptidoglycan cell wall layer. Cleavage of this FRET substrate by sortase reveals the fluorescent signal, Abs/Em = 340/490 nm.)

HY-P5528

2Abz-SLGRKIQIK(Dnp)-NH2	Peptides	Others
2Abz-SLGRKIQIK(Dnp)-NH2 is a biological active peptide. (This peptide is a second complement component (C2), the physiological substrate for the proenzyme Cls, first complement component. The complement system is a central component of host defense but can also contribute to the inflammation seen in pathological conditions. The C1s protease of the C1 complex initiates the host defense pathway. This peptide employs 2Abz/Dnp FRET pair for quantitation of complement enzyme activity.)

HY-P4919

Mca-SEVNLDAEFK(Dnp)	Beta-secretase	Others
Mca-SEVNLDAEFK(Dnp) is a Beta-secretase 1 (BACE-1) peptide FRET substrate, containing the 'Swedish' Lys-Met/Asn-Leu mutation of the amyloid precursor protein (APP) β-secretase cleavage site. Cleavage at -Leu-Asp- of Mca-SEVNLDAEFK(Dnp) liberates the highly fluorescent 7-methoxycoumarin (Mca) fragment from the proximity quenching effect of the 2,4-dinitrophenyl (Dnp) internal quencher resulting in a large and easily detectable increase in fluorescence intensity.

HY-P5415

DABCYL-GABA-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS	HIV	Others
DABCYL-GABA-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS is a biological active peptide. (DABCYL-GABA-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS is also called HIV protease substrate I in some literature. It is widely used for the continuous assay for HIV protease activity. The 11-kD protease (PR) encoded by the human immunodeficiency virus 1 (HIV-1) is essential for the correct processing of viral polyproteins and the maturation of infectious virus, and is therefore a target for the design of selective acquired immunodeficiency syndrome (AIDS) therapeutics. The FRET-based fluorogenic substrate is derived from a natural processing site for HIV-1 PR. Incubation of recombinant HIV-1 PR with the fluorogenic substrate resulted in specific cleavage at the Tyr-Pro bond and a time-dependent increase in fluorescence intensity that is linearly related to the extent of substrate hydrolysis. The fluorescence quantum yields of the HIV-1 PR substrate in the FRET assay increased by 40.0- and 34.4-fold, respectively, per mole of substrate cleaved. Because of its simplicity and precision in the determination of reaction rates required for kinetic analysis, this substrate offers many advantages over the commonly used HPLC or electrophoresis-based assays for peptide substrate hydrolysis by retroviral PRs. Abs/Em = 340nm/490nm.)

HY-P5377

Abz-HPGGPQ-EDDnp Cathepsin K substrate	Ser/Thr Protease	Others
Abz-HPGGPQ-EDDnp (Cathepsin K substrate) is a biological active peptide. (Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin K is the lysosomal cysteine protease involved in bone remodeling and resorption. It has potential as a drug target in autoimmune diseases and osteoporosis.This FRET peptide can be used to monitor selectively cathepsin K activity in physiological fluids and cell lysates. Abz-HPGGPQ-EDDnp [where Abz represents o-aminobenzoic acid and EDDnp represents N -(2, 4-dinitrophenyl)-ethylenediamine], a substrate initially developed for trypanosomal enzymes, is efficiently cleaved at the Gly-Gly bond by cathepsin K. This peptide is resistant to hydrolysis by cathepsins B, F, H, L, S and V, Ex/Em=340 nm/420 nm.)

Cat. No.	Product Name	Target	Research Area

HY-P99900

Sevirumab MSL-109	CMV	Infection
Sevirumab (MSL-109) is a human IgG1 neutralizing monoclonal antibody against cytomegalovirus (CMV). Sevirumab recognizes CMV gH complexes and inhibits CMV replication with an EC₅₀ of 0.3 μg/mL .

Cat. No.	Product Name	Chemical Structure

HY-W027544S

MCA-d3
MCA-d₃ (7-Methoxycoumarin-4-acetic acid₃) is the deuterium labeled MCA (HY-W027544) . MCA is a Coumarin (HY-N0709) derivative. MCA quantitates platelet-activating factor (PAF) by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities .

Cat. No.	Product Name		Classification

HY-D2579

Sulfo DBCO-UBQ-2		DBCO
Sulfo DBCO-UBQ-2 is a click chemistry reagent combining a dark quencher, UBQ-2, with a polyaromatic-azo backbone, offering no native emission. UBQ-2 effectively quenches fluorescence in the 560-670 nm range, ideal for qPCR probes and FRET applications with orange to far-red dyes .

HY-D2167

AF 568 alkyne		Alkynes
AF 568 alkyne is a derivative of the orange fluorescent dye AF 568. AF 568 has an absorption wavelength of 590-720 nm (FRET) and an emission wavelength of 515-720 nm. AF 568 alkyne can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) via the Alkyne group and molecules containing Azide groups. To achieve specific coupling of dye labels and biomolecules .

Cat. No.	Product Name		Classification

HY-160276

DOPE-PEG-Fluor 555,MW 5000		Pegylated Lipids Fluorescent Lipids
DOPE-PEG-Fluor 555, MW 5,000 is consist of a DOPE phospholipid which is an unsaturated phospholipid and a Fluor 555 dye which is a bright orange cyanine dye that can be used in fluorescence microscopy, FRET and other in vivo imaging techniques.

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