Scopolamine hydrochloride  (Synonyms: (-)-Scopolamine hydrochloride; Hyoscine hydrochloride)

Scopolamine (Hyoscine) hydrochloride is a high-affinity muscarinic antagonist that can cross the blood-brain barrier. Scopolamine hydrochloride competitively antagonizes 5-HT₃ receptors with an IC₅₀ of 2.09 μM. Scopolamine hydrochloride can induce cognitive and memory deficits in animals. Scopolamine hydrochloride can be used in the research of preventing postoperative nausea and vomiting, motion sickness, nervous system diseases, etc^{[1][2][3][4][5]}.

Scopolamine hydrochloride blocks nicotinic acetylcholine receptors (IC₅₀ = 928 μM) and increases the expression of α7 nACh receptors. Scopolamine hydrochloride displays concentration-dependent competition with 0.6 nM [³H]Granisetron, yielding an average pK_i of 5.17 (K_i = 6.76 μM). Concentration-dependent competition of fluorescently labeled form of Granisetron (HY-B0071) with Scopolamine hydrochloride gives an average pK_i of 5.31 (K_i = 4.90 μM).Scopolamine hydrochloride blocks muscarinic receptors and induces a cognitive deficit^[1].
Scopolamine (0.1-3 mM; for 24 hours) hydrochloride induces cytotoxicity, ROS generation and apoptosis of SH-SY5Y cells^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Scopolamine (1 mg/kg; i.p.; once) induces memory impairment associated with attenuation of cholinergic neurotransmission, as well as an increases of processes connected with oxidative stress in the brain in mice^[7].
Scopolamine can be used to induce memory impairment models^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

339.81

C₁₇H₂₂ClNO₄

55-16-3

O=C([C@@H](C1=CC=CC=C1)CO)O[C@H]2C[C@H]3[C@@H]4O[C@@H]4[C@H](N3C)C2.[H]Cl

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Lochner M, et al. The muscarinic antagonists Scopolamine and atropine are competitive antagonists at 5-HT3 receptors. Neuropharmacology. 2016 Sep;108:220-8.  [Content Brief]

  [2]. O ET, et al. COGNITIVE-ENHANCING PROPERTIES OF MORINDA LUCIDA (RUBIACEAE) AND PELTOPHORUM PTEROCARPUM (FABACEAE) IN SCOPOLAMINE-INDUCED AMNESIC MICE. Afr J Tradit Complement Altern Med. 2017 Mar 1;14(3):136-141.  [Content Brief]

  [3]. Pattanashetti LA, et al. Evaluation of neuroprotective effect of Quercetin with Donepezil in Scopolamine-induced amnesia in rats. Indian J Pharmacol. 2017 Jan-Feb;49(1):60-64.  [Content Brief]

  [4]. Yadang FSA, et al. Scopolamine-Induced Memory Impairment in Mice: Neuroprotective Effects of Carissa edulis (Forssk.) Valh (Apocynaceae) Aqueous Extract. Int J Alzheimers Dis. 2020 Aug 31;2020:6372059.  [Content Brief]

  [5]. Klinkenberg I, et al. The validity of scopolamine as a pharmacological model for cognitive impairment: a review of animal behavioral studies. Neurosci Biobehav Rev. 2010 Jul;34(8):1307-50.  [Content Brief]

  [6]. Nicha Puangmalai, et al. Neuroprotection of N-benzylcinnamide on scopolamine-induced cholinergic dysfunction in human SH-SY5Y neuroblastoma cells. Neural Regen Res. 2017 Sep;12(9):1492-1498.   [Content Brief]

  [7]. Barbara Budzynska, et al. Effects of imperatorin on scopolamine-induced cognitive impairment and oxidative stress in mice. Psychopharmacology (Berl). 2015 Mar;232(5):931-42.   [Content Brief]