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  5. Scopolamine hydrochloride

Scopolamine hydrochloride  (Synonyms: (-)-Scopolamine hydrochloride; Hyoscine hydrochloride)

Cat. No.: HY-B2065
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Scopolamine (Hyoscine) hydrochloride is a high-affinity muscarinic antagonist that can cross the blood-brain barrier. Scopolamine hydrochloride competitively antagonizes 5-HT3 receptors with an IC50 of 2.09 μM. Scopolamine hydrochloride can induce cognitive and memory deficits in animals. Scopolamine hydrochloride can be used in the research of preventing postoperative nausea and vomiting, motion sickness, nervous system diseases, etc.

For research use only. We do not sell to patients.

Scopolamine hydrochloride

Scopolamine hydrochloride Chemical Structure

CAS No. : 55-16-3

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Scopolamine hydrochloride Related Antibodies

Top Publications Citing Use of Products

2 Publications Citing Use of MCE Scopolamine hydrochloride

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Description

Scopolamine (Hyoscine) hydrochloride is a high-affinity muscarinic antagonist that can cross the blood-brain barrier. Scopolamine hydrochloride competitively antagonizes 5-HT3 receptors with an IC50 of 2.09 μM. Scopolamine hydrochloride can induce cognitive and memory deficits in animals. Scopolamine hydrochloride can be used in the research of preventing postoperative nausea and vomiting, motion sickness, nervous system diseases, etc[1][2][3][4][5].

IC50 & Target[1]

5-HT3 Receptor

2.09 μM (IC50)

In Vitro

Scopolamine hydrochloride blocks nicotinic acetylcholine receptors (IC50 = 928 μM) and increases the expression of α7 nACh receptors. Scopolamine hydrochloride displays concentration-dependent competition with 0.6 nM [3H]Granisetron, yielding an average pKi of 5.17 (Ki = 6.76 μM). Concentration-dependent competition of fluorescently labeled form of Granisetron (HY-B0071) with Scopolamine hydrochloride gives an average pKi of 5.31 (Ki = 4.90 μM).Scopolamine hydrochloride blocks muscarinic receptors and induces a cognitive deficit[1].
Scopolamine (0.1-3 mM; for 24 hours) hydrochloride induces cytotoxicity, ROS generation and apoptosis of SH-SY5Y cells[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Scopolamine hydrochloride Related Antibodies

Cell Viability Assay[6]

Cell Line: Human neuroblastoma SH-SY5Y cell line
Concentration: 0.1-3 mM (usually 2 mM)
Incubation Time: 24 hours
Result: Exposure to 1-3 mM for 24 hours significantly decreased human neuroblastoma SH-SY5Y cell viability in a dose-dependent manner. In all subsequent experiments, 2 mM resulted in 50.6% loss in cell viability.
In Vivo

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Scopolamine (1 mg/kg; i.p.; once) induces memory impairment associated with attenuation of cholinergic neurotransmission, as well as an increases of processes connected with oxidative stress in the brain in mice[7].
Scopolamine can be used to induce memory impairment models[4].

Induction of Memory Impairment
Background
Scopolamine is a selective acetylcholine receptor antagonist, which reduces the effectiveness of acetylcholin at the synapse through ocupation of the receptor sites on the post-synaptic membrane.
Specific Modeling Methods
Mice: Mus musculus Swiss • 2 months old • 25-30 g
Administration: 1 mg/kg/day for 7 days
Note
Modeling Indicators
Behavior-T-maze test: Increased latency time to enter the preferred arm. Reduced number of entries and time staying in the preferred arm, while increased number of entries and time staying in the discrminated arm.
Behavior-Locomotion activity in open field: Decreased time spent in the centre of the open field. Decreased number of crossing and rearing.
Behavior-Object recognition: Increased time to discover and stay with the familiar object. Decreased number of exploration of the novel object.
Molecular changes: Decreased level of glutathione. Increased activity of acetylcholinesterase in brain.
Correlated Product(s): Streptozotocin (HY-13753); Lipopolysaccharide (HY-D1056)
Opposite Product(s): Tacrine (HY-111338); Rivastigmine (HY-17368); Donepezil (HY-14566)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Naive male Swiss mice weighing 20-25[7]
Dosage: 1 mg/kg
Administration: Administered intraperitoneally (i.p.); to measure the memory acquisition processes, Scopolamine was administered 20 min before the pretest; to measure the memory consolidation processes, Scopolamine was administered immediately after the pretest
Result: There was a significant decrease in the index of latency (IL) value as compared with the saline-treated mice, at the used dose impaired acquisition of memory and learning.
Clinical Trial
Molecular Weight

339.81

Formula

C17H22ClNO4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C([C@@H](C1=CC=CC=C1)CO)O[C@H]2C[C@H]3[C@@H]4O[C@@H]4[C@H](N3C)C2.[H]Cl
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (147.14 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9428 mL 14.7141 mL 29.4282 mL
5 mM 0.5886 mL 2.9428 mL 5.8856 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.9428 mL 14.7141 mL 29.4282 mL 73.5705 mL
5 mM 0.5886 mL 2.9428 mL 5.8856 mL 14.7141 mL
10 mM 0.2943 mL 1.4714 mL 2.9428 mL 7.3571 mL
15 mM 0.1962 mL 0.9809 mL 1.9619 mL 4.9047 mL
20 mM 0.1471 mL 0.7357 mL 1.4714 mL 3.6785 mL
25 mM 0.1177 mL 0.5886 mL 1.1771 mL 2.9428 mL
30 mM 0.0981 mL 0.4905 mL 0.9809 mL 2.4524 mL
40 mM 0.0736 mL 0.3679 mL 0.7357 mL 1.8393 mL
50 mM 0.0589 mL 0.2943 mL 0.5886 mL 1.4714 mL
60 mM 0.0490 mL 0.2452 mL 0.4905 mL 1.2262 mL
80 mM 0.0368 mL 0.1839 mL 0.3679 mL 0.9196 mL
100 mM 0.0294 mL 0.1471 mL 0.2943 mL 0.7357 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Scopolamine hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Scopolamine55-16-3(-)-ScopolamineHyoscinemAChR5-HT ReceptorMuscarinic acetylcholine receptorSerotonin Receptor5-hydroxytryptamine ReceptorAlzheimerdiseaseacetylcholineapoptosisacetylcholinesteraseoxidativestressneuronal5-HT3MuscarinicAnxietyCognitionMemoryDepressionHippocampusAmygdalaInhibitorinhibitorinhibit

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