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Scopolamine hydrobromide trihydrate  (Synonyms: Hyoscine hydrobromide trihydrate)

Cat. No.: HY-W010892 Purity: 99.71%
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Scopolamine (Hyoscine) hydrobromide trihydrate is a high-affinity muscarinic antagonist that can cross the blood-brain barrier. Scopolamine hydrobromide trihydrate competitively antagonizes 5-HT3 receptors with an IC50 of 2.09 μM. Scopolamine hydrobromide trihydrate can induce cognitive and memory deficits in animals. Scopolamine hydrobromide trihydrate can be used in the research of preventing postoperative nausea and vomiting, motion sickness, nervous system diseases, etc.

For research use only. We do not sell to patients.

Scopolamine hydrobromide trihydrate Chemical Structure

Scopolamine hydrobromide trihydrate Chemical Structure

CAS No. : 6533-68-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
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Based on 14 publication(s) in Google Scholar

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Description

Scopolamine (Hyoscine) hydrobromide trihydrate is a high-affinity muscarinic antagonist that can cross the blood-brain barrier. Scopolamine hydrobromide trihydrate competitively antagonizes 5-HT3 receptors with an IC50 of 2.09 μM. Scopolamine hydrobromide trihydrate can induce cognitive and memory deficits in animals. Scopolamine hydrobromide trihydrate can be used in the research of preventing postoperative nausea and vomiting, motion sickness, nervous system diseases, etc[1][2][3][4][5].

In Vitro

receptors (IC50 = 928 μM) and increases the expression of α7 nACh receptors. Scopolamine hydrobromide trihydrate displays concentration-dependent competition with 0.6 nM [3H]Granisetron, yielding an average pKii = 6.76 μM). Concentration-dependent competition of fluorescently labeled form of Granisetron (HY-B0071) with Scopolamine hydrobromide trihydrate gives an average pKi of 5.31 (Ki = 4.90 μM). Scopolamine hydrobromide trihydrate blocks muscarinic receptors and induces a cognitive deficit[1].
Scopolamine (0.1-3 mM; for 24 hours) hydrobromide trihydrate induces cytotoxicity, ROS generation and apoptosis of SH-SY5Y cells[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[6]

Cell Line: SH-SY5Y cell line
Concentration: 0.1-3 mM (usually 2 mM)
Incubation Time: 24 hours
Result: Exposure to 1-3 mM for 24 hours significantly decreased human neuroblastoma SH-SY5Y cell viability in a dose-dependent manner. In all subsequent experiments, 2 mM resulted in 50.6% loss in cell viability.
In Vivo

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Scopolamine (1 mg/kg; i.p.; once) induces memory impairment associated with attenuation of cholinergic neurotransmission, as well as an increases of processes connected with oxidative stress in the brain in mice[7].
Scopolamine can be used to induce memory impairment models[4].

Induction of Memory Impairment
Background
Scopolamine is a selective acetylcholine receptor antagonist, which reduces the effectiveness of acetylcholin at the synapse through ocupation of the receptor sites on the post-synaptic membrane.
Specific Modeling Methods
Mice: Mus musculus Swiss • 2 months old • 25-30 g
Administration: 1 mg/kg/day for 7 days
Note
Modeling Indicators
Behavior-T-maze test: Increased latency time to enter the preferred arm. Reduced number of entries and time staying in the preferred arm, while increased number of entries and time staying in the discrminated arm.
Behavior-Locomotion activity in open field: Decreased time spent in the centre of the open field. Decreased number of crossing and rearing.
Behavior-Object recognition: Increased time to discover and stay with the familiar object. Decreased number of exploration of the novel object.
Molecular changes: Decreased level of glutathione. Increased activity of acetylcholinesterase in brain.
Correlated Product(s): Streptozotocin (HY-13753); Lipopolysaccharide (HY-D1056)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Naive male Swiss mice weighing 20-25 g[7]
Dosage: 1 mg/kg
Administration: Administered intraperitoneally (i.p.); to measure the memory acquisition processes, Scopolamine was administered 20 min before the pretest; to measure the memory consolidation processes, Scopolamine was administered immediately after the pretest
Result: There was a significant decrease in the index of latency (IL) value as compared with the saline-treated mice, at the used dose impaired acquisition of memory and learning.
Clinical Trial
Molecular Weight

438.31

Formula

C17H28BrNO7

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C([C@@H](C1=CC=CC=C1)CO)O[C@H]2C[C@H]3[C@@H]4O[C@@H]4[C@H](N3C)C2.[H]Br.[H]O[H].[H]O[H].[H]O[H]

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (285.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2815 mL 11.4075 mL 22.8149 mL
5 mM 0.4563 mL 2.2815 mL 4.5630 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.89%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2815 mL 11.4075 mL 22.8149 mL 57.0373 mL
5 mM 0.4563 mL 2.2815 mL 4.5630 mL 11.4075 mL
10 mM 0.2281 mL 1.1407 mL 2.2815 mL 5.7037 mL
15 mM 0.1521 mL 0.7605 mL 1.5210 mL 3.8025 mL
20 mM 0.1141 mL 0.5704 mL 1.1407 mL 2.8519 mL
25 mM 0.0913 mL 0.4563 mL 0.9126 mL 2.2815 mL
30 mM 0.0760 mL 0.3802 mL 0.7605 mL 1.9012 mL
40 mM 0.0570 mL 0.2852 mL 0.5704 mL 1.4259 mL
50 mM 0.0456 mL 0.2281 mL 0.4563 mL 1.1407 mL
60 mM 0.0380 mL 0.1901 mL 0.3802 mL 0.9506 mL
80 mM 0.0285 mL 0.1426 mL 0.2852 mL 0.7130 mL
100 mM 0.0228 mL 0.1141 mL 0.2281 mL 0.5704 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Scopolamine hydrobromide trihydrate
Cat. No.:
HY-W010892
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