2. Anti-infection
  3. Bacterial
  4. RW3

RW3 (MP196) is a small cationic antimicrobial hexapeptide. RW3 targets the bacterial cytoplasmic membrane and inhibits cellular respiration and cell wall synthesis. RW3 exhibits high bioactivity against Gram-positive bacteria such as Bacillus subtilis 168 (MIC of 2 µg/mL). RW3 causes shrinking of murine erythrocytes. RW3 can be used in antimicrobial and antifungal research.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

RW3

RW3 Chemical Structure

CAS No. : 359632-11-4

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Based on 1 publication(s) in Google Scholar

RW3 Related Antibodies

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Description

RW3 (MP196) is a small cationic antimicrobial hexapeptide. RW3 targets the bacterial cytoplasmic membrane and inhibits cellular respiration and cell wall synthesis. RW3 exhibits high bioactivity against Gram-positive bacteria such as Bacillus subtilis 168 (MIC of 2 µg/mL). RW3 causes shrinking of murine erythrocytes. RW3 can be used in antimicrobial and antifungal research[1].

In Vitro

RW3 (16 h) shows decent activity against Gram-positive bacteria like Bacillus subtilis 168 (MIC of 2 µg/mL) and some MRSA/VISA strains, but weak activity against Gram-negative bacteria such as Pseudomonas aeruginosa PA01 (MIC of >64 µg/mL)[1].
RW3 (0.09-200 µg/mL; 72 h) shows no significant cytotoxicity against NRK-52E rat kidney epithelial cells and CCRF-CEM human T-cell lymphoblast cells[1].
RW3 (10 ng-500 µg/mL; 1-24 h) does not induce NF-κB activation in RT4 bladder epithelial cells[1].
RW3 (250-500 µg/mL) causes shrinking of murine erythrocytes at 250 µg/mL and severe morphological changes plus lysis at 500 µg/mL, with 14% and 23% hemolysis respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RW3 Related Antibodies
In Vivo

RW3 (5-25 mg/kg; i.v.) causes excitement and indisposition (recovered within 25 min) at 5-10 mg/kg, and hind limb paralysis plus death within minutes at 25 mg/kg in mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

1044.22

Formula

C51H69N19O6
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Arg-Trp-Arg-Trp-Arg-Trp-NH2
Sequence Shortening

RWRWRW-NH2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (23.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.9577 mL 4.7883 mL 9.5765 mL
5 mM 0.1915 mL 0.9577 mL 1.9153 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.9577 mL 4.7883 mL 9.5765 mL 23.9413 mL
5 mM 0.1915 mL 0.9577 mL 1.9153 mL 4.7883 mL
10 mM 0.0958 mL 0.4788 mL 0.9577 mL 2.3941 mL
15 mM 0.0638 mL 0.3192 mL 0.6384 mL 1.5961 mL
20 mM 0.0479 mL 0.2394 mL 0.4788 mL 1.1971 mL
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RW3 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RW3359632-11-4RW 3RW-3BacterialAntimicrobial and antifungalgram-positive bacteriaplasma membraneInhibitorinhibitorinhibit

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