USP4 Protein, Human (Active, sf9, His, Flag)

USP4 Protein, Human (Active, sf9, His, Flag) is the recombinant human-derived USP4, expressed by sf9 insect cells , with His, Flag labeled tag. ,

USP4 is a deubiquitinating enzyme that removes conjugated ubiquitin from target proteins. Its functions include: deubiquitinating PDPK1; deubiquitinating TRIM21; deubiquitinating the receptor ADORA2A, thereby increasing the amount of functional receptor at the cell surface; deubiquitinating HAS2; deubiquitinating RHEB in response to EGF signaling, promoting mTORC1 signaling; potentially regulating mRNA splicing through deubiquitination of the spliceosomal protein PRPF3, which may destabilize interactions within the U4/U6.U5 snRNP; and possibly playing a role in ER quality control. by similar: USP4's deubiquitination activity targets diverse substrates, including signaling proteins, receptors, and spliceosomal components, highlighting its broad regulatory roles in cellular processes.

Active

Human

Sf9 insect cells

His;Flag

Q13107-1 (A2-N963)

7375

Partial

Greater than 80% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 5% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.