UBE3A Protein, Human

UBE3A Protein, Human is the recombinant human-derived UBE3A, expressed by E. coli.

UBE3A is an E3 ubiquitin-protein ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme as a thioester and transfers it to substrates. Identified substrates include BMAL1, ARC, LAMTOR1, RAD23A, RAD23B, MCM7 (involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, UBE3A may function as a cellular quality control ubiquitin ligase by facilitating the degradation of cytoplasmic misfolded proteins and promotes its own degradation in vivo. It plays a critical role in circadian clock regulation by ubiquitinating the core clock component BMAL1, leading to its proteasomal degradation. UBE3A acts as a transcriptional coactivator of progesterone receptor PGR upon progesterone activation. By similarity, it regulates synaptic development by mediating ubiquitination and degradation of ARC and controls synaptic remodeling in neurons by ubiquitinating and degrading LAMTOR1, thereby limiting mTORC1 signaling and activity-dependent synaptic remodeling. UBE3A also synergizes with WBP2 to enhance PGR activity.

Human

E. coli

Tag Free

Q05086-1 (E2-L875)

7337

Partial

Approximately 102.7 kDa

Greater than 80% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.