2. Recombinant Proteins
  3. Ubiquitin Related Proteins
  4. Ubiquitin Enzymes
  5. E2 Enzymes
  6. UBE2N/Ubc13 Protein, Human (sf9)

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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Top Publications Citing Use of Products

  • Technical Parameters

  • Properties

  • Documentation

Species

Human
Source

Sf9 insect cells
Tag

Tag Free
Accession

P61088 (A2-I152)
Gene ID

7334
Molecular Construction
N-term
UbcH13 (A2-I152)
Accession # P61088
C-term
Protein Length

Partial
Synonyms
Ubiquitin-conjugating enzyme E2 N; UbcH13
AA Sequence

AGLPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAMNNI

Molecular Weight

Approximately 16.4 kDa, based on SDS-PAGE under reducing conditions
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Solution.
Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 1 mM DTT, 5% glycerol.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

N/A.
Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping

Shipping with dry ice.
Documentation
Handling Instructions (2659 KB)

UBE2N/Ubc13 Protein, Human (sf9) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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UBE2N/Ubc13 Protein, Human (sf9)
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