UBA1 Protein, Human (sf9)

The UBA1 protein initiates ubiquitin conjugation, marking proteins for degradation in the ubiquitin-proteasome system. UBA1 Protein, Human (sf9) is the recombinant human-derived UBA1 protein, expressed by Sf9 insect cells , with no tag.

The UBA1 protein plays a pivotal role in cellular processes as it catalyzes the initial step in ubiquitin conjugation, marking cellular proteins for degradation through the ubiquitin-proteasome system, as documented in several studies. This enzymatic activity involves the activation of ubiquitin by initially adenylating its C-terminal glycine residue with ATP. Subsequently, UBA1 links this activated ubiquitin to the side chain of a cysteine residue in E1, forming a ubiquitin-E1 thioester and releasing free AMP. The indispensable function of UBA1 extends to critical cellular processes, including the formation of radiation-induced foci, timely DNA repair, and the response to replication stress. Furthermore, UBA1 plays a role in promoting the recruitment of TP53BP1 and BRCA1 at DNA damage sites, emphasizing its significance in orchestrating DNA repair pathways and maintaining genomic integrity.

Sf9 insect cells

P22314 (S2-R1058)

7317

Full Length of Mature Protein

Approximately 117 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.