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  4. U2AF2 Protein, Human (His, Strep)

U2AF2 Protein, Human (His, Strep) is the recombinant human-derived U2AF2, expressed by E. coli , with Strep, His labeled tag. ,

For research use only. We do not sell to patients.

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Description

U2AF2 Protein, Human (His, Strep) is the recombinant human-derived U2AF2, expressed by E. coli , with Strep, His labeled tag. ,
Background

U2AF2 plays a role in pre-mRNA splicing and 3'-end processing. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD) and binding to pre-mRNA, it may couple transcription with splicing. In muscle tissue, it induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion and regulates TNNT2 exon 5 inclusion through competition with MBNL1. During spliceosome assembly, it preferentially binds to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4. It is also required for mRNA nuclear export, even for intron-less genes. Additionally, it represses the splicing of MAPT/Tau exon 10 and positively regulates pre-mRNA 3'-end processing by recruiting the CFIm complex to cleavage and polyadenylation signals.
Species

Human
Source

E. coli
Tag

Strep;His
Accession

P26368-1 (V140-A342 )
Gene ID

11338
Protein Length

Partial
Synonyms
U2AF65
Predicted Molecular Mass
25.1 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

U2AF2 Protein, Human (His, Strep) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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U2AF2 Protein, Human (His, Strep)
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